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A method to improve the detection recovery rate of cefquinome residues in food of animal origin and reduce the matrix effect

A technology of cefquinoxime and matrix effect is applied in the field of improving the detection recovery rate of cefquinoxime residues in animal-derived food and reducing the matrix effect, so as to achieve the effects of reducing the matrix effect, improving the recovery rate and ensuring safety

Inactive Publication Date: 2019-02-01
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The samples were not dehydrated before extraction, and the recovery rate of cefquinome was measured to have a large room for improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this example, cefquinome was selected as the analyte, and pig muscle, liver fresh sample and freeze-dried sample were respectively used as experimental samples, and the comparative experiment of the addition recovery rate of cefquinome was carried out.

[0023] 1. Sample processing

[0024] (1) Preparation of blank addition of fresh samples

[0025] Accurately weigh 5.0±0.05g of the blank sample into a centrifuge tube, add an appropriate amount of standard solution, vortex and mix, and let it stand for more than 20 minutes to prepare blanks with three concentration levels of 1 / 2MRL (maximum residue limit), MRL and 2MRL. Fresh samples, namely: muscle 25, 50, 100 μg / kg; liver 50, 100, 200 μg / kg; to be extracted.

[0026] (2) Preparation of blank addition freeze-dried samples

[0027] Prepare a blank with the same concentration as above, add fresh samples, pre-cool and vacuum freeze-dry, take out the samples, mash them immediately to powder, and wait for extraction. ...

Embodiment 2

[0067] In this example, pig liver fresh samples and freeze-dried samples were selected as experimental samples to evaluate the matrix effect of cefquinome.

[0068] 1. Sample processing

[0069] (1) Preparation of freeze-dried powder samples

[0070] With embodiment 1.

[0071] (2) Preparation of blank matrix solution

[0072] The blank pig liver fresh sample or freeze-dried sample was extracted and purified according to the same method as in Example 1 to obtain a blank matrix solution of fresh or freeze-dried pig liver sample.

[0073] (3) Preparation of pure solvent standard solution and blank matrix matching standard solution

[0074] Add cefquinome to ultrapure water and the prepared blank matrix solution to prepare pure solvent standard solutions (50, 100 and 200 μg / L) and blank matrix matching standard solutions (50, 100 μg / L) containing the same concentration of cefquinome and 200μg / L i.e. 50, 100 and 200μg / kg)

[0075] 2. Sample testing

[0076] Adopt high-perfor...

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PUM

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Abstract

The invention discloses a method for improving the detection recovery rate of cefquinome residue in animal-derived food and reducing a substrate effect. According to the method, moisture is effectively removed through freeze drying; the recovery rate of the cefquinome residue in the animal-derived food is obviously improved; the substrate effect is reduced, so that a detection result is more accurate; the reasonable clinical application of cefquinome in veterinary medicine can be more effectively monitored; the safety of the animal-derived food for people is guaranteed.

Description

technical field [0001] The invention relates to a method for improving the detection recovery rate of cefquinome residue in animal source food and reducing its matrix effect. Background technique [0002] Cefquinome (cefquinome) is the fourth-generation cephalosporin animal-specific drug, which was developed by the German company Hoechst in the 1980s. In 1993, cefquinome sulfate was approved for marketing for the first time. Suspensions and ointments developed by British Intervet were launched in 1994. The domestic cefquinome raw material and its injection were approved and registered by the Ministry of Agriculture in 2008 as a national second-class new veterinary drug. Cefquinome has the characteristics of broad antibacterial spectrum, strong antibacterial activity, rapid absorption, safety and high efficiency, and has been widely used in the clinical treatment of various diseases such as swine and cattle respiratory diseases, dairy cow mastitis, and metritis. In order t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王立琦王忠王丽霞杨帆鲍光明王小莺刘宝生
Owner JIANGXI AGRICULTURAL UNIVERSITY
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