275 results about "Animal source foods" patented technology

The invention discloses a special physiological regulator for maternal animals, and a preparation method thereof, and belongs to the field of feed additive. A technical scheme of the physiological regulator comprises: carrying out crushing and screening for dandelion, leonurus, licorice root and angelica; adding soybean meal and/or wheat bran; adding water to carry out immersing; adjusting pH value of the resulting mixture; carrying out a sterilization; inoculating probiotics to carry out a fermentation after cooling, followed by stoving after completing the fermentation to obtain the physiological regulator. With the prepared physiological regulator for the maternal animals, immunity and disease resistance of the maternal animals are raised, incidence rate of genital system infection is effectively reduced, feed inversion rate is increased, mammogenesis is improved, milk yield is raised, incidence rate of mammitis is reduced, and milk quality is improved. The physiological regulator has characteristics of quick onset, stable and reliable effect, no toxic and side-effect, no pollution, no residue, and no drug resistance generating, and meets requirements of safe feed additive and assurance of foods of animal origin. In addition, the physiological regulator for the maternal animals can be adopted as a veterinary drug through raising using dosage of the physiological regulator, and provides a substantial curative effect for prevention and cure of the mammitis.

The invention discloses a high-throughput detection method for 99 residual veterinary drugs in animal-derived food. The detection method is characterized by comprising the following concrete steps: (1) carrying out extraction and purification on 99 residual veterinary drugs having substantially different physicochemical properties and belonging to eight kinds of common veterinary drugs through one-step pretreatment based on carrier-assisted liquid-liquid extraction technology; (2) preparation of a mixed standard solution and a matrix standard curve; and (3) determining the concentrations of 99 residual veterinary drugs in a to-be-detected solution by using ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry. According to the invention, the pretreatment and instrumental analysis process of the method is directed at compounds with different physicochemical properties, so the method has the advantages of good compatibility, high detection efficiency, good operability and low detection cost, and the detection limit of the method can meet requirements of all the test objects.

The invention relates to a liquid-mass database for detecting residual drugs in animal-derived food and a use method thereof; preparation of the database includes the following steps: (1) preparing a standard work solution; (2) treating before analyzing, namely using an extraction purification technology combining fast enzymolysis and fast solid phase extraction (SPE) to treat to-be-detected samples before analyzing; (3) performing one-time sample introduction chromatographic analysis; and (4) building the liquid-mass spectral database.

The invention relates to a liquid chromatography for detecting anabolic hormone drug residue in animal-derived food, which is characterized by first sampling: animal musculature is taken off the fat and connective tissue, then minced and evenly ground, and the sample is weighed; extracting: anabolic hormone extract is extracted from the weighed sample by methanol ultrasonic extraction, the extract is evaporated to dryness in water bath through a rotary evaporator, and the residue is dissolved by methanol aqueous solution; purifying: C18 Solidoid extraction column on the extract is carried out solid phase extraction; and testing by devices: the filtrate is tested by opposite phase high efficiency liquid chromatography, quantified by external reference method-peak area, and then carried out with binary gradient elution. In the invention, the detected sample is complex biological sample; the established method can complete one detection in about one hour and is simple and fast, with reliable sensitivity and low cost; the method can analyze more veterinary hormone drug species than the synchronous usage of the existing GC or HPLC methods with easier operation, and has lower cost than the existing GC/MS and LC/MS or LC/MS/MS methods with low solvent toxicity.

The invention provides an animal-derived food pathogen identification and drug-resistant and toxic gene detection composite chip. The invention provides a probe for identifying the animal-derived food pathogen, the pathogen drug-resistant gene and/or drug toxicity gene. The probe consists of single-chain DNA (deoxyribonucleic acid) molecules shown by the sequence 1 to sequence 171. Experiment results show that the animal-derived food pathogen identification and drug-resistant and toxic gene detection composite chip provided by the invention can effectively achieve the integral goal of simultaneously identifying the bacteria, the toxicity of the bacteria and the drug-resistant gene.

The invention provides a kit for detecting diethylstilbertrol and detection method thereof, belonging the the time resolution fluoroimmunoassay (TRFIA) technical field, capable of detecting the content of diethylstilbestrol (DES) in animal-source food, blood, urine and feedstuff. The kit uses the TRFIA to detect the DES and the mark immune response is the base of the detection. A micropore plate is coated by DES-carrier protein and added with DES standard or sample and then added with DES antibody. The free DES and the DES-carrier protein on the micropore plate complete the DES antibody and the DES antibody which is not bonded with the free DES or the DES-carrier protein is cleared away and added with the EU3+-goat anti-rabbit antibody, after labeling the immune reaction, the non-bonded EU3+-goat anti-rabbit antibody is cleared away. After adding the reinforcement liquid, the fluorescence intensity cps is detected using a time resolution fluorescence instrument and the fluorescence intensity is inversely proportional to the DES concentration in sample and the content of DES in sample can be determined according to the standard curve. The DES detection kit has features of simple structure, convenient use, cheapness, high sensitivity up to 1ng/mL.

The invention discloses a method for preparing hapten, antigen and antibody of 5-methylmorpholine-3-amino-2-oxazolidone derivatives. The hapten obtained by designing and preparing the 5-methylmorpholine-3-amino-2-oxazolidone derivatives is similar to counterparts of the 5-methylmorpholine-3-amino-2-oxazolidone derivatives in molecular structure, spatial chemistry and electron distribution. The linking arm in the hapten structure is not easy to induce and generate 'arm antibody', and the organism is easier to identify as a saturate carbon chain of a certain length is used; the antigen which is prepared by hapten cross coupling carrier protein ensures the organism to produce the antibody of high tilter and good specificity in accordance with the 5-methylmorpholine-3-amino-2-oxazolidone derivatives; and the antibody is used for detecting the residue of 5-methylmorpholine-3-amino-2-oxazolidone in animal source food with quick response and stable result.

The invention provides a liquid chromatography-mass spectrometry method for rapidly detecting the content of beta-agonists. The liquid chromatography-mass spectrometry method comprises the following steps: pretreating a sample, namely, sequentially adding water and an organic extracting solution in the sample for extracting a beta-receptor stimulant; then adding a salting-out agent and a water removing agent to ensure that the organic extracting solution and a water phase demix, centrifuging to obtain a first supernate, adding an adsorbing agent in the first supernate for purification, centrifuging to obtain a second supernate, concentrating, re-dissolving and filtering the second supernate to prepare a sample to be detected; and then detecting the beta-receptor stimulant contained in an animal-derived food by adopting an ultra-high performance liquid chromatography-triple quadrupole mass spectrometer. According to the sample pretreatment method suitable for liquid chromatography-mass spectrometry detection, provided by the invention, interfering molecules are removed through extraction and purification so that high accuracy is achieved in sample detection.

The invention belongs to the technical field of biological pharmacy, in particular to a bacterial strain green chromogenic streptomyces viridochromogenes SDSL1119 for producing antibiotic avilamycin special for an animal and a method for producing the avilamycin by the bacterial strain. The bacterial strain is collected in a CGMCC on July 21st, 2009 with the collection number of CGMCCNo.3198. Themethod comprises the steps of oblique plane culture, seed culture and fermentation culture. The invention has the advantages of environmental-friendly production process, high product bioactivity, favorable antibiosis effect and no residue and cross medicine resistance and can effectively prevent animal diseases and ensure the safety of animal source foods.

The invention discloses an immune colloidal gold detecting card of carbendazin and a preparation method of the immune colloidal gold detecting card, and relates to the technical field of animal source food veterinary drug residue detection. A test strip in a shell of the detecting card is composed of a PVC glue plate, a sample cushion, a colloidal gold conjugate pad, a coating film and a water absorbing cushion, wherein a colloidal gold film is a glass cellulose membrane containing carbendazin monoclonal antibodies, the coating film is a nitrocellulose film and is provided with a line T and a line C, the line T is wrapped by carbendazin protein conjugates, and the line C is wrapped by goat-anti-mouse IgG antibodies. The detecting card can be effectively used for rapidly detecting carbendazin and is convenient to use, rapid and accurate in result.

The present invention relates to 'method for examining solid-phase dispersion -high performance liquid chromatogram of residues substrate of sulfonamides in animal derived food' which belongs to the field of food safety-veterinary drug residues. The method comprises of chromatogram examining steps of substrate solid-phase dispersion and high performance liquid, wherein, the mobile phase of sodium dihydrogen phosphate-acetonitrile is 50mmol/L in high performance liquid chromatogram examining method, the volume ratio of sodium dihydrogen phosphate and acetonitrile is 70:30, because the sodium dihydrogen phosphate solution is buffer solution which has a certain PH buffer capability and makes the PH value stable, the stability of pH value of HPLC mobile phase is ensured, and repeatability and reproducibility of analysis result are ensured also, at the same time, the classical process of extraction, separation, concentration and purification are simplified, the operation is simple, the milk combination phenomenon is eliminated. The method of present invention has the merits of ideal precision and sensitivity, and environment protecting by using the less toxicity organic solvent eluting analyte employed.

The present invention discloses an irradiation freshness-keeping technology of raw and fresh animal source food. The technology comprises the following steps: firstly, cold and fresh meat is taken, the taken meat is cut into several meat blocks with a thickness of 15-20 cm; secondly, the meat blocks are sub-bag packed; thirdly, the packed meat blocks are placed under the irradiation of an electron beam irradiation device in a manner of single layer under a irradiation dose of 1.4-2.2 kGy and a irradiation dose rate of 2200-2600 Gy/s; and fourthly, the irradiated meat blocks are subjected to a cold storage. By regulating the dose rate, the quality of the irradiated cold and fresh meat is greatly improved, the microorganisms can be killed quickly, the original texture, color, nutrition, flavor, etc. of the meat blocks can be better maintained, and the meat blocks have a long storage period. The technology is simple in operation and high-efficient, at the same time improves the function of the dose rate, reduces the irradiation damage, is green, safe and convenient, widely achieves the irradiation of agricultural products, keeps quality and freshness, and extends shelf life.

The invention discloses an immune colloidal gold detect card of chlorpromazine, and a preparation method thereof, and belongs to the technical field of animal-derived food veterinary drug residue detection. A test strip of an outer housing of the immune colloidal gold detect card is composed of a PVC slab, a sample pad, a colloidal gold conjugate pad, a coating membrane, and an absorbent pad; a colloidal gold membrane is a glass cellulose membrane containing chlorpromazine monoclonal antibodies; the coating membrane is a nitrocellulose memebrane, and is provided with a T line and a C line; the T line contains chlorpromazine protein conjugates via coating; and the C line contains goat anti-mouse IgG antibodies via coating. The immune colloidal gold detect card is used for rapid detection of chlorpromazine effectively, and is convenient and rapid for using; and detection results are accurate.

The invention relates to a method for rapidly screening out cephalosporins drug residues from animal muscle tissue. The method comprises the following steps of performing sample processing and detection analysis, manufacturing a standard curve, and utilizing inspection data obtained in the sample processing and detection analysis process for calculating the concentration of a cephalosporins compound in a to-be-tested sample through the standard curve. According to the method, by means of an ultra-high performance liquid chromatography-quadrupole-track trap mass spectrum combination system, the technology for screening out cephalosporins drug residues from animal derived food is successfully built, and 44 cephalosporins drug residues can be screened out from the animal muscle tissue; in addition, the detection method is easy to implement, good in repeatability, high in analysis speed and high in resolution ratio, the resolution ratio is within 0.17-1.23 ppm, the recovery rate of all the 44 target objects is stable, the recovery rate is within 69.20-129.00%, and the method can be effectively used for screening out cephalosporins drug residues from the animal source food.

The invention relates to a traditional Chinese medicine composition for preventing and treating the coccidiosis of poultry. The traditional Chinese medicine composition respectively comprises the following components: a radix dichroae extract, a radix bupleuri extract, a cytromium rhizome extract, a torilis anthriscus extract, a kuhseng extract, a Korean pulsatilla extract, a bigfruit elm extract, a sweet wormwood extract, a rangooncreeper fruit extract, a Japanese pagodatree extract, an astragalus root extract, a herba ecliptae extract, a isatis leaf extract, an angelica extract, a radix platycodi extract, a rehmannia root extract, a weeping forsythia extract, a purslane extract, a heba euphorbiae humifusae extract and a plantain extract. The invention has lower cost, convenient use, no toxic or side effect and no medicine residue and is the traditional Chinese medicine composition for preventing and treating the coccidiosis of the poultry, which conforms to the requirement of a safe animal remedy and guarantees the safety of an animal source food.

The invention relates to a determination method of polymyxins E residue content in animal source food by superhigh liquid chromatogram-tandom mass spectrometry. The method comprises the following steps: adding an extract in a sample to be measured, fully whirling and extracting, high-speed freezing and centrifuging, then obtaining a sample liquid after filtering and purifying; weighing a polymyxins E standard sample, dissolving by methanol to take it as a storing solution, then further, diluting by methanol to obtain a standard working solution possessing a concentration gradient; detecting the sample by using a superhigh liquid chromatogram-tandom quadrupole liquid chromatography-mass spectrometer; reading out the concentration value of polymyxins E on a standard curve, and calculating the result of polymyxins E of the sample. According to the invention, the determination method of polymyxins E residue in animal source food is rapid and effective, the relative average deviation is less than 10%. Therefore, the method provided in the invention is reliable and easy to enforce for detecting polymyxins E residue in animal source food, which is capable of satisfying the requirments of research and production.

The invention discloses a preparation method of a ciprofloxacin and enrofloxacin residue freeze-dried powder standard sample in carp muscle, which takes the carp as a research object to research a carp muscle material object standard sample containing ciprofloxacin and enrofloxacin of certain concentration in a breeding mode of adding target medicine. The key production control technology of the material object standard sample disclosed by the invention is mastered and comprises the control of content level, evenness, stability, constant value and the like, and the standard sample is produced and prepared in a standardization and scale mode. The standard sample is used for detection quality control and detection capability evaluation for residue of veterinary drug in animal-derived food and has an important significance on food safety detection.

The invention discloses a chemical luminescence enzyme-linked immunoassay kit of gatifloxacin, comprising a kit body, an enzyme-marked plate and a reagent which are arranged in the kit body; wherein, all the holes of the enzyme-marked plate are coated with coating antigen; the coating antigen is prepared by the coupling of the gatifloxacin and ovalbumin; the reagent comprises a polyclonal antibody of the gatifloxacin, an enzyme-marked second antibody (namely, the antibody of horseradish peroxidase-marked goat anti-rabbit), a gatifloxacin series standard solution, a concentrated phosphate buffer, a concentrative cleaning solution and a luminescence solution. The kit has the advantages of high sensitiveness, good repeatability, simpleness, fastness and exactness; and compared with traditional colorimetric ELISA method, the sensitiveness can be improved by one magnitude and the method is expected to play an import role in the gatifloxacin residual detection of animal source foods (such as milk samples and animal tissue samples) and a urine sample.

The invention relates to a traditional Chinese medicine compound pharmaceutical composition and a preparation method and application thereof. The traditional Chinese medicine compound pharmaceutical composition contains clove, Salvia miltiorrhiza, dodder, epimedium, fructus cnidii, radix codonopsitis, radix astragali, angelica, rhizoma ligustici wallichii, phellodendron, radix scutellariae, rhizoma coptidis, Gardenia, ebony, hawthorn and kaki calyx. The traditional Chinese medicine compound pharmaceutical composition has the efficacies of replenishing Qi to invigorate the spleen, warming uterus and tonifying Yang, tonifying kidney and consolidating, nourishing blood and tonifying Qi, activating blood flow and removing blood stasis and clearing heat and detoxicating and has the functions of preventing and curing the inflammations of the fallopian tubes of the laying fowls. The traditional Chinese medicine compound pharmaceutical composition has definite curative effect, rapid action, safe use and no toxic and side effects or residuals, meets the demand of safety of animal-derived foods, is easy to realize industrial production and does not have pollution on the environment. The invention also provides a preparation method of the traditional Chinese medicine compound pharmaceutical composition and the application of preventing and curing the inflammations of the fallopian tubes of the laying fowls and improving the laying function. The traditional Chinese medicine compound pharmaceutical composition can be a powder. The invention also provides a feed containing the traditional Chinese medicine compound pharmaceutical composition.

The invention relates to a medicament for improving production performance of pigs and a preparation method thereof. The medicament comprises the following components in percentage by weight: 15-25% of carvacrol, 10-15% of anise oil, 10-15% of tangerine peel oil, 6-10% of garlic essential oil, 5-7% of cinnamic aldehyde, 3-5% of thymol and 23-51% of white carbon black. The medicament provided by the invention is convenient to use, can be directly added to animal feed without toxic/side effects or medicament residues, and conforms to the requirements of the safe veterinary medicaments as well as the requirements for ensuring the safety of animal-derived food. The invention can enhance the palatability of feed, make pigs happier, improve the intestinal mucosa function, increase the digestibility, obviously promote the growth, improve the immunity, reduce the stress reaction, decrease the sickness rate of pigs and greatly improve the quality of meat.

The invention discloses a molecular engram integral cylinder and its process method for detecting gentamicin, streptomycin and neomycin residue in animal food.The method adopts gentamicin, streptomycin and neomycin as molecular modular and combining in original position to process gentamicin, streptomycin and neomycin molecular engram integral cylinder. The stuff in cylinder is a crosslinked polymers of determined range functional group with fixed hole size and shape, which has memory function for modular molecular spatial structure of gentamicin, streptomycin and neomycin.

The invention relate to a immunity antibody for detecting polyether antibiotic remains and the application of the antibody. With the monensin sodium salt as half antigen, first reform the half antigen and then couple the reformed half antigen with protein producing artificial immunogen, and obtain multi clone antigen by immunizing the New Zealand rabbit or single clone antigen by immunizing the small mouse, cell fusion, sieving, clone cultivation. The obtained antigen can be used for test the animal source food on polyether antibiotic remains. The advantages of the invention are that the antigen obtained by immunizing animals with the coupling product of reformed monensin sodium salt and protein, has got specificity on polyether antibiotic medical which can be applied on polyether antibiotic remain detecting in animal source food. The detection adopted with this invention is fast and convenience.

The invention relates to a full-nutrient formula food based on an optimal phytochemical composition and a preparation method thereof. 14 natural food materials are used as main raw materials and a formula and the preparation method of the full-nutrient formula food ensure full nutrient of the food and a certain ratio of phytochemical. The main food materials comprise 11.9 percent of staple food grains (brown rice, whole wheat and oats), 13.6 percent of coarse cereals (corns, millets and soybeans), 33.2 percent of animal non-staple food (lean meat, eggs and milk products), 34.1 percent of fruits (apples) and vegetables (carrots and cauliflowers) and 7.2 percent of other materials. The full -nutrient formula food has the advantages that high-quality ecological unprocessed grains, green fruits and vegetables, animal source food materials and the like are used as basic components and nutritional vectors; the population dietary nutrition supply quantity recommendation standard is used as the foundation; the full-nutrient formula food has both full nutrients and an effect of regulating oxidative damage. When a human body takes in a certain quantity of the food, all the daily physiological requirements and the requirements for regulating the antioxidant function can be met.