Method and liquid-mass database for detecting residual drugs in animal-derived food

Abstract

The invention relates to a liquid-mass database for detecting residual drugs in animal-derived food and a use method thereof; preparation of the database includes the following steps: (1) preparing a standard work solution; (2) treating before analyzing, namely using an extraction purification technology combining fast enzymolysis and fast solid phase extraction (SPE) to treat to-be-detected samples before analyzing; (3) performing one-time sample introduction chromatographic analysis; and (4) building the liquid-mass spectral database.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a liquid-mass database for detecting residual drugs in animal-source food and a method for using the same. Background technique [0002] Foods of animal origin account for a considerable proportion of international trade in agricultural products, and regulations and restrictions on drug residues in foods of animal origin are becoming more and more stringent. Taking Japan’s “Positive List” system implemented in May 2006 as an example, 236 types of veterinary drugs and feedstuffs Additives have set specific limit standards, and in the field of domestic food safety, from the early "chloramphenicol, nitrofuran" to the later "malachite green, clenbuterol", frequent food safety incidents caused by drug residues have become prominent The technical support capability of my country's food safety supervision and control system is insufficient, and it is urgent to establish a r...

AI Technical Summary

Problems solved by technology

However, whether in terms of method development, validation and standardization, or the improvement of regulations, there are many difficulties in the simultaneous detection of multiple types of residues:

[0003] (1) The chemical properties of different types of veterinary drugs are very different, the polar coverage is wide, and the residue forms in different matrices are different. Individual veterinary drugs still need derivatization to be effectively detected. It is difficult to achieve an effective breakthrough;

[0004] (2) The regulations have different limit requirements and use regulations for different veterinary drugs (banned drugs and restricted drugs), and the multi-type residue detection and verification rules have not yet been perfected;

[0005] (3) The blank matrix required for method development is difficult to obtain, and the preparation of mixed standard solutions is difficult;

[0007] 1) Only MS / MS data, no chromatographic system information characteristics;

[0008] 2) No online information feature;

[0009] 3) There is no unified pretreatment method coupled with instrumental analysis;

[0010] 4) Lack of matrix standard data

[0011] Therefore, under the existing conditions, it is difficult to effectively realize the rapid screening analysis of multiple types of residues based on liquid mass spectrometry library technology.

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