68results about How to "Good data repeatability" patented technology

The invention discloses an establishing method of a circular RNA high-throughput sequencing library and a kit thereof. The establishing method sequentially comprises the following steps: (S1) extracting total RNA from a sample; (S2) removing DNA in the sample; (S3) detecting and evaluating the quality of the total RNA in the sample, and determining that the RNA quality meets a requirement; (S4) removing rRNA; (S5) removing linear RNA; (S6) constructing the circular RNA library for high-throughput sequencing. The establishing method of the circular RNA high-throughput sequencing library, provided by the invention, is high in efficiency, stable, low in residual ratio of the rRNA, high in circular RNA detection efficiency, good in data reproducibility, and high in sequencing result verifying success rate, and is especially applicable to an FFPE tissue and other samples with poor RNA quality.

The invention discloses an image analysis method and apparatus for skin lustrousness, so that the skin lustrousness difference of people and the improvement effects of products on the skin lustrousness are effectively reflected and visual evaluation results of people are better reflected. The technical scheme is that the method comprises: acquiring a digital image of the skin of a testee under the same light source condition; setting a to-be-analyzed region on the acquired skin image; calculating frequency distribution of pixels for the to-be-analyzed region; setting extraction conditions of a highlight part; and calculating a pixel occupation ratio of the highlight part extracted according to the set extraction conditions, and taking the pixel occupation ratio as a skin lustrousness index.

The present invention provides a device and method for measurement of a light passing surface normal of an electro-optic crystal and an optical axis of an electro-optic crystal. The main components of the device comprise: a linear polarized light laser, a first lens, a small aperture diaphragm, a second lens, a spectroscope, a plane mirror, a receiving screen, a third lens, a one-dimensional precision electric displacement stand, a fourth lens, a linear polaroid, a fifth lens, an image detector and a computer, and a photoelectric autocollimator and a sheet of parallel plate glass with 1'' of depth of parallelism are required for installation and regulation of a light path, etc. The device and method for measurement of the light passing surface normal of the electro-optic crystal and the optical axis of the electro-optic crystal realize non-contact nondestructive detection of the electro-optic crystal to be detected so as to overcome a series of problems that a crystal is easy to generate scratches, a measurement device is difficult to build and a conoscopic interference pattern is difficult to analyze in the current method. The third lens and the fourth lens change different F numbers to perform measurement of electro-optic crystals with different thicknesses. The data repeatability of measurement is good, and compared to the measurement data of a current X-ray crystal direction finder, the relative error is within 30''.

The invention provides a sulfur recovery catalyst activity evaluating device and a test method, and belongs to the field of testing or analyzing a material by testing the chemical or physical property of the material. The device is characterized in that 1, a heat insulation type electric heating furnace is replaced by a blowing-type constant-temperature electric heating case (6), a reactor (7) is arranged in the blowing-type constant-temperature electric heating case (6); 2, a water pre-heater is saved, the distilled water after being measured is directly gasified in a water gasifying electric heating pre-heating coil (16) arranged in the blowing-type constant-temperature electric heating case (6); 3, an alkali liquor absorbing tank (12) is added; and 4, a computer control system (11) is provided. The sulfur recovery catalyst activity evaluating device and the test method provided by the invention have the characteristics of safety and stability in running, low temperature difference of a catalyst bed layer of the reactor, constant reaction temperature, exact and reliable test data, good repeatability, safety and environmental protection and convenience in use.

The invention discloses a detection reagent for lipoprotein-associated phospholipase A2 and a preparation method thereof. The preparation method is characterized by comprising the following steps: a, weighing, by weight, 0.2 to 0.6 part of 1-myristoyl-2-(4-nitrophenyl succinyl)phosphatidylcholine, 0.1 to 0.5 part of citric acid, 0.04 to 0.2 part of sodium 1-nonane sulfonate, 0.06 to 0.20 part of ethylene diamine tetraacetic acid and 0.03 to 0.09 part of vitamin E; and b, dissolving the above-mentioned ingredients in 100 parts by weight of purified water and adjusting the pH value of an obtained solution to 2.50 to 5.00 by using hydrochloric acid with a concentration of 5M so as to prepare the detection reagent for lipoprotein-associated phospholipase A2. The detection reagent provided by the invention has the advantages of low preparation cost, good stability, easy preservation, good data repeatability and high detection sensitivity.

The invention relates to the technical field of bolt non-destructive detecting, and specifically discloses a bolt ultrasonic detecting device.Thebolt ultrasonic detecting device comprises a bolt, anda guiding mechanism is arranged on the outer side of the top end of the bolt, the guiding mechanism comprises an inner rail frame and an outer rail frame, the inner rail frame and the outer rail frameare both of ring structures and are concentrically and parallelly distributed, a plurality of connecting rods are fixedly mounted between the inner rail frame and the outer rail frame, a first rail is mounted on the top of the inner rail frame, a second rail is fixedly mounted on the top end of the outer rail frame, a fixing mechanism is arranged at the bottom of the guiding mechanism,a sliding mechanism is arranged on the top of the guiding mechanism,a clamping mechanism is fixedly mounted on one side of the sliding mechanism, an ultrasonic probe is clamped on the clamping mechanism, and theultrasonic probe is located above the bolt. According to thebolt ultrasonic detecting device,the function of automatic detection is realized, data repeatability is good,detection precision is high, the position of the ultrasonic probe can be adjusted, thus the position of the ultrasonic probe is precisely controlled, and the detection precision is improved.

The invention provides a method for determining the encapsulation rate of propofol medium/long chain fat emulsion injection. The method provided by the invention combines ultrafiltration centrifugation and high performance liquid chromatography detection to determine the encapsulation rate of propofol medium/long chain fat emulsion injection, and is especially suitable for propofol medium/long chain fat emulsion injection. The method also has the advantages of good reproducibility, good accuracy, verifiability, short time and low experiment cost, and can reflect the actual encapsulation condition of propofol medium/long chain fat emulsion injection more truly and accurately.

The invention relates to an adjustable n-shaped mixer. The mixer is in an n shape. A motor connecting shaft which is connected with a motor is arranged on the upper portion of the mixer. A cuboid screw type mixing blade adjuster is welded to the lower end of the motor connecting shaft. Tooth sliding blocks which are located in a slideway and engaged with the slideway are pushed by rotating a screw, so that mixing blades fixed to the lower portions of the tooth sliding blocks are driven, and the distance between the two mixing blades is adjusted to be adapted to different cylindrical containers. Each mixing blade is in the shape of an inverted L, and in order to improve the mixing effect, the included angle between the transverse direction of the mixing part of each mixing blade and the tangent line of a rotating arc of the mixing part of the mixing blade is 35 degrees. The purpose of even mixing is achieved through revolution of the mixing blades and a lower revolution speed, and thus side effects of mechanical shearing on a polymer solution are reduced to the maximum extent in the preparation process. The adjustable n-shaped mixer is mainly used for stirring in the indoor polymer solution preparation process.

The invention discloses a group biotoxicity detection method and belongs to the field of a biological analysis and detection technology. According to the method, a specific biological bacterium is cultivated, benzoin dimethyl ether is used as a photoinitiator, and the biological bacterium is irradiated with ultraviolet rays to give out light; then, initial luminosity factor and post luminosity factor of the photogenic bacterium are measured by a photometric measuring instrument. The higher the luminosity factor is, the higher the toxicity detection ratio is. Thus, biotoxicity detection is realized. According to the invention, the biochemiluminescence toxicity detection method is invented in allusion to present technical defects, problems such as low toxicity detection rate, low work efficiency and the influence of strains are solved, and work efficiency and toxicity detection rate are raised. The detection method provided by the invention has characteristics of fast analysis speed, low cost, no influence of strains and the like.

The invention relates to the technical field of immunodetection, and provides a kit for simultaneous detection of four depression markers and a preparation method thereof, the preparation method is as follows: antibody coating is prepared; enzyme label plates prepared in the first step are respectively poured, closed, washed, air-dried, and stored for standby use; a sample is loaded, the enzyme label plates are put into an incubation oscillator for incubation, then a detection antibody is added; enzyme SA HRP is added into plate holes for continuing incubation; CCD imager is used for filming and imaging, the image IDV value can be calculated by combination of image analysis software, according to the IDV value, a standard curve is drawn, and the sample concentration result is calculated; and softMax regression model and Excel table are used for calculation and statistical analysis of the sample concentration result. The preparation method solves effectively the problem of complicated process, operation disadvantage and low sensitivity.

The invention relates to a nondestructive detection method of the storage ageing performance of a propellant/liner bonding interface, used for spaceflight fuel nondestructive detection. The method comprises the following steps: sample preparation and parameter detection, data analysis and mathematic modeling, and parameter calculation and data processing, wherein the sample preparation and parameter detection step comprises contrastive sample preparation and contrast parameter establishment which are used for macroscopic detection, and detection sample preparation and infrared spectrum detection which are used for microanalysis; infrared spectrogram analysis and establishment of a mathematical relationship are carried out in the data analysis and mathematic modeling step; and in the parameter calculation and data processing step, peak separation software of an infrared spectrometer is used to acquire data, and the data is substituted to the mathematical relationship, and parameter calculation is carried out to acquire a performance parameter. The method adopts a tiny amount sampling mode, infrared spectrum detection and mathematic modeling to realize spectrogram analysis and data processing in order to obtain the bonding performance index of the propellant/liner bonding interface. The method has the characteristics of convenient detection process, good data repeatability, reliable parameter analysis and optimal data processing.

The invention discloses a method for determining the niobium content in a titanium-niobium alloy. The method comprises: preparing a Ti45Nb sample solution; taking the Ti45Nb sample solution, and developing the color with a PAR solution; measuring the absorbance of the developed solution at a wavelength of 520-530 nm on a spectrophotometer; preparing a niobium standard solution; preparing a titanium base solution; preparing a series of standard solutions for establishing the niobium concentrations from 0.00%-0.25*10<-3>% of a curve, wherein the solutions are corresponding to the niobium concentrations from 0-50% in the sample; establishing a working curve, wherein a series of standard solutions of S6 are subjected to color development, the absorbance values are measured, and the working curve using the concentrations as a abscissa and the absorbance values as an ordinate is established; and dissolving the Ti45Nb sample, developing the color, determining the absorbance value, and obtaining the corresponding concentration value in the established working curve by using the determined absorbance value so as to obtain the niobium content in the Ti45Nb memory alloy material. According to the present invention, the method has advantages of stable color development, satisfactory working curve, and good data repeatability.

The invention discloses a method for detecting ultraviolet differential nitrogen dioxide gas. The method comprises the following steps: collecting sample gas; making a standard curve: introducing 99.999% nitrogen into the gas chamber at a flow rate of 2L/min, collecting an N2 spectrum and storing data, then introducing NO2 standard gas, and collecting NO2 spectrum data; and by utilizing a Lambert-Beer law, calculating the molar absorption coefficient of NO2 based on a 100% NO2 standard absorption curve, then, substitutingthe molar absorption coefficient of NO2 into data of the first several groups of spectrums, and calculating the actual concentration, wherein it is obtained according to multiple experiments that deviation exists between the actual concentration and the theoretical concentration, the actually calculated concentration needs to be corrected, and after correction through a third-order function, the actual concentration and the theoretical concentration are within a required error range. According to the method, the concentration of NO2 in the gas is measured by utilizing an ultraviolet spectrum differential technology, and the method has good data accuracy and repeatability and wide application range.

The invention discloses a liquid recognition and detection automatic sample injector with adjustable temperature. The sample injector is composed of a frame, a sample injection assembly, a detection assembly, a temperature-adjusting assembly and a control system; the sample injection assembly is provided with a supporting plate, a supporting frame, a water bath pool, a locating plate, a driving synchronizing wheel, a sample injection stepping motor, a synchronizing belt, a driven synchronizing wheel and the like; the water bath pool is fixed on the driven synchronizing wheel, the bottom of the water bath pool is provided with a locating gage mark, the supporting plate, the supporting frame and the locating plate are fixed in the water bath pool after being connected rigidly; the temperature-adjusting assembly is provided with a heater, a refrigerator, a refrigerating heat exchanger, a stirrer, a stirring motor, a temperature control bracket and the like; a spiral heater and the refrigerating heat exchanger are concentrically fixed below the temperature control bracket, the stirring motor is mounted above the temperature control bracket, the stirrer is fixed on the stirring motor, and a through axis of the stirrer is coincident with the central line of the heater. The automatic sample injector provided by the invention can be used for carrying out constant-temperature detectionon samples under a set constant temperature condition at different environment temperatures; the temperature is adjustable, the samples are automatically injected and the testing precision is high.

The invention relates to a construction method and application of a sequencing library of circular small non-coding RNA (Ribonucleic Acid). The construction method comprises the following steps: (1) culturing cells; (2) extracting total RNA (Ribonucleic Acid) from the cells; (3) extracting RNA by using a small RNA extraction kit, and removing 28S and 18S rRNA; (4) further removing 5S and 5.8 S rRNA by an RAP method; (5) removing linear RNA; and (6) constructing the sequencing library of the circular small non-coding RNA. The constructed sequencing library of the circular small non-coding RNA has the advantages of few rRNA residues, high detection efficiency of the circular RNA, high sequencing flux, better data repeatability and high success rate of verification of a sequencing result. The whole method is simple and convenient to operate, low in cost and time consumption, high in stability and very good in repeatability and reliability.

The invention discloses a sample heating and post-cooling device for detecting propellant decomposition gas. The sample heating and post-cooling device comprises a tank body, a cap, a cooling pipe, a gas production port and a heating furnace, the tank body is a barrel body, a heat conduction column is arranged in the tank body, the cap is provided with an air hole, and the cap and the tank body are assembled into the sample tank by tightening threads. The left end of the spring-shaped cooling pipe is connected with the air hole of the sample tank cap, and the right end of the cooling pipe is communicated with the sampling port. The sample tank filled with the propellant powder into a heating hole of a heating furnace, heating is performed at 90 DEG C for 3 hours, and gas in the sample tank is extracted for detection. Compared with an original device, the device disclosed by the invention has the advantages of uniform sample heating, high sample temperature rising speed, capability of rapidly cooling decomposition gas to be detected and the like, the precision of a detection result is improved, and the sample heating and post-cooling time is shortened.