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Group biotoxicity detection method

A technology of biological toxicity and detection method, which is applied in the direction of biological testing, chemiluminescence/bioluminescence, material inspection products, etc., can solve the problems of low work efficiency, easy to be affected by bacteria, low toxicity detection rate, etc., and achieve convenient testing, Fast test speed and good data repeatability

Inactive Publication Date: 2015-09-09
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems of low toxicity detection rate, low work efficiency and being easily affected by bacteria in the above method, and proposes a chemiluminescence toxicity detection method; a specific biological bacteria is cultivated to emit light, and when there is When there are toxic substances, the intensity of light will become stronger. Use a photometer to measure the initial and post-test luminous rate, so as to determine whether the toxicity detection rate has increased. It has the characteristics of fast analysis speed, relatively low cost, and is not affected by bacteria species.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042] (1) get a cultured biological bacteria to test;

[0043] (2) 4 mg of biological bacteria powder is mixed with 120 mL of hydrogel, and 1 g of benzoin dimethyl ether is taken as a photoinitiator, and the color is blue;

[0044] (3) Drop the mixed biological bacteria on a test paper material with a length of 20 cm and a width of 10 cm, and irradiate it with a purple light for 10 seconds;

[0045] (4) Put the biological bacteria irradiated by the purple light into 3% normal saline for recovery. After the recovery is complete, use a photometric instrument to measure the initial light intensity of the luminescent bacteria, and make a record, and then put the luminescent biological bacteria into the waiting Measure the water body, place it for 2 minutes, take out the luminescence of the luminescent bacteria after the test, make a record, and compare the luminosity before and after the test.

[0046] If the luminous rate of the biological bacteria of the present invention is c...

example 2

[0048] (1) get a cultured biological bacteria to test;

[0049] (2) 4.5 mg of biological bacteria powder is mixed with 130 mL of hydrogel, and 1.5 g of benzoin dimethyl ether is taken as a photoinitiator, and the color is blue;

[0050] (3) Drop the mixed biological bacteria on a test paper material with a length of 20 cm and a width of 10 cm, and irradiate it with a purple light for 13 seconds;

[0051](4) Put the biological bacteria irradiated by the purple light into 3% normal saline for recovery. After the recovery is complete, use a photometric instrument to measure the initial light intensity of the luminescent bacteria, and make a record, and then put the luminescent biological bacteria into the waiting Measure the water body, place it for 2.5 minutes, take out the luminescence of the luminescent bacteria after the test test, make a record, and compare the test luminosity before and after.

[0052] If the luminous rate of the biological bacteria of the present inventio...

example 3

[0054] (1) get a cultured biological bacteria to test;

[0055] (2) 5 mg of biological bacteria powder is mixed with 150 mL of hydrogel, and 2 g of benzoin dimethyl ether is taken as a photoinitiator, and the color is blue;

[0056] (3) Drop the mixed biological bacteria on a test paper material with a length of 20 cm and a width of 10 cm, and irradiate it with a purple light for 15 seconds;

[0057] (4) Put the biological bacteria irradiated by the purple light into 3% normal saline for recovery. After the recovery is complete, use a photometric instrument to measure the initial light intensity of the luminescent bacteria, and make a record, and then put the luminescent biological bacteria into the waiting Measure the water body, place it for 3 minutes, take out the luminescence of the luminescent bacteria after the test test, make a record, and compare the test luminosity before and after.

[0058] If the luminous rate of the biological bacteria of the present invention is ...

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Abstract

The invention discloses a group biotoxicity detection method and belongs to the field of a biological analysis and detection technology. According to the method, a specific biological bacterium is cultivated, benzoin dimethyl ether is used as a photoinitiator, and the biological bacterium is irradiated with ultraviolet rays to give out light; then, initial luminosity factor and post luminosity factor of the photogenic bacterium are measured by a photometric measuring instrument. The higher the luminosity factor is, the higher the toxicity detection ratio is. Thus, biotoxicity detection is realized. According to the invention, the biochemiluminescence toxicity detection method is invented in allusion to present technical defects, problems such as low toxicity detection rate, low work efficiency and the influence of strains are solved, and work efficiency and toxicity detection rate are raised. The detection method provided by the invention has characteristics of fast analysis speed, low cost, no influence of strains and the like.

Description

technical field [0001] The invention discloses a group biological toxicity detection method, which belongs to the technical field of biological analysis and detection. Background technique [0002] Biological toxicity refers to the toxicity of the sample to organisms. It is divided into acute toxicity and chronic toxicity, usually expressed as a semi-lethal concentration. It is applied in environmental protection, clinical medicine, tap water, customs and public security. At present, common biosensors are divided into fish and shellfish. , algae, fleas and luminescent bacteria. [0003] With the development of modern industry, the use of chemical substances is increasing day by day, which has caused more and more serious pollution to the aquatic ecosystem on which human beings depend, and sudden environmental pollution accidents have occurred from time to time, such as man-made poisoning, natural pollution, etc. Sudden changes in water quality caused by disasters, especiall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/50
Inventor 赵远徐波蔡强孙向武陈文艳肖娴仇爱峰俞洁孙娜
Owner CHANGZHOU UNIV
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