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Detection reagent for lipoprotein-associated phospholipase A2 and preparation method thereof

A technology for detecting reagents and phospholipases, which is used in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemically reacting materials. Problems such as poor repeatability, to achieve the effect of easy storage, good data repeatability and good stability

Active Publication Date: 2013-04-24
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reagents currently used to detect Lp-PLA2 are mainly based on the principle of enzyme-linked immunosorbent assay, which uses the principle of antigen-antibody specific binding. The product has disadvantages such as cumbersome operation, poor data repeatability, and high price.

Method used

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  • Detection reagent for lipoprotein-associated phospholipase A2 and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0014] (1) Weigh 0.2 grams of 1-tetradecanoyl-2-(4-nitrophenylsuccinyl) lecithin, 0.1 grams of citric acid, 0.04 grams of sodium 1-nonanesulfonate, and 0.06 grams of ethylenediaminetetraacetic acid , 0.03 g of vitamin E, dissolved in 100 ml of purified water, adjusted to pH 2.50 with 5 mol / L hydrochloric acid, and prepared as a detection reagent for lipoprotein-related phospholipase A2.

Embodiment 2

[0016] (2) Weigh 0.24 grams of 1-tetradecanoyl-2-(4-nitrophenylsuccinyl) lecithin, 0.14 grams of citric acid, 0.056 grams of sodium 1-nonanesulfonate, and 0.074 grams of ethylenediaminetetraacetic acid , vitamin E 0.036 g, dissolved in 100 ml of purified water, adjusted to pH 2.75 with 5 mol / L hydrochloric acid, and formulated as a detection reagent for lipoprotein-related phospholipase A2.

Embodiment 3

[0018] (3) Weigh 0.28 grams of 1-tetradecanoyl-2-(4-nitrophenylsuccinyl) lecithin, 0.18 grams of citric acid, 0.072 grams of sodium 1-nonanesulfonate, and 0.088 grams of ethylenediaminetetraacetic acid , vitamin E 0.042 g, dissolved in 100 ml of purified water, adjusted to pH 3.00 with 5 mol / L hydrochloric acid, and made into a lipoprotein-related phospholipase A2 detection reagent.

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Abstract

The invention discloses a detection reagent for lipoprotein-associated phospholipase A2 and a preparation method thereof. The preparation method is characterized by comprising the following steps: a, weighing, by weight, 0.2 to 0.6 part of 1-myristoyl-2-(4-nitrophenyl succinyl)phosphatidylcholine, 0.1 to 0.5 part of citric acid, 0.04 to 0.2 part of sodium 1-nonane sulfonate, 0.06 to 0.20 part of ethylene diamine tetraacetic acid and 0.03 to 0.09 part of vitamin E; and b, dissolving the above-mentioned ingredients in 100 parts by weight of purified water and adjusting the pH value of an obtained solution to 2.50 to 5.00 by using hydrochloric acid with a concentration of 5M so as to prepare the detection reagent for lipoprotein-associated phospholipase A2. The detection reagent provided by the invention has the advantages of low preparation cost, good stability, easy preservation, good data repeatability and high detection sensitivity.

Description

technical field [0001] The invention relates to the field of preparation of diagnostic reagents, in particular to a detection reagent for lipoprotein-associated phospholipase A2 and a preparation method thereof. Background technique [0002] Lipoprotein-associated phospholipase A2 (abbreviated as Lp-PLA2) is a member of the phospholipase A2 superfamily and belongs to PLA2-VIL. It contains 441 amino acid residues and has a relative molecular mass of about 45,400. Human plasma Lp-PLA2 is synthesized and secreted by macrophages, lymphocytes and mast cells, exists in large quantities in atherosclerotic sites, and can be regulated by inflammatory mediators, which has the effect of promoting atherosclerosis. Lp-PLA2 is a novel inflammatory response marker that plays a role in several major stages of AS (ankylosing spondylitis). Lp-PLA2 is mainly produced by macrophages and lymphocytes. In the early studies on Lp-PLA2 and AS[2-3], Lp-PLA2 can hydrolyze inflammatory factors such as...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 李民友
Owner ZYBIO INC
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