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Liquid chromatography for synchronously detecting 15 anabolic hormone residues in food

An anabolic hormone, liquid chromatography technology, applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of few types and no simultaneous detection method of steroid anabolic hormones, etc., and achieves popularization of instruments, sensitive and reliable cost, Easy to use effect

Active Publication Date: 2009-10-07
上海国矗生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few types of drugs to be tested at the same time, and there are no steroid anabolic hormones and β 2 -Research on simultaneous detection method of receptor agonists
For complex biological samples, the detection technology of multiple hormone drug residues is still in the stage of exploratory research

Method used

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  • Liquid chromatography for synchronously detecting 15 anabolic hormone residues in food

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: A liquid chromatography method for simultaneously detecting 15 kinds of anabolic hormone drug residues in food of animal origin according to the present invention adopts the following steps:

[0015] Sampling: Mince the beef after removing fat and other connective tissues and grind it into a homogenous slurry with a food grinder;

[0016] Extraction: Ultrasonic extraction of anabolic hormones from ground beef with methanol; the mass-volume ratio (m / v, g / mL) of beef mass to methanol analytically pure A.R is 1:6, extracted twice, and each extraction time is 0.5h , to obtain an anabolic hormone extract; the extract was evaporated to dryness in a water bath at 40° C. with a rotary evaporator, and the residue was dissolved with a certain amount of 20% (v / v) aqueous methanol solution;

[0017] Purify: C 18 The solid-phase extraction column was activated with 3mL methanol and 5mL water, and the sample extract was passed through the C at a speed of 2mL / min. 18 C...

Embodiment 2

[0019] Embodiment 2: A liquid chromatography method of the present invention for simultaneously detecting 15 kinds of anabolic hormone drug residues in food of animal origin, adopts the following steps:

[0020] Sampling: Mince the chicken breast after removing fat and other connective tissues, and grind it into a homogenous slurry with a food grinder;

[0021] Extraction: Ultrasonic extraction of anabolic hormones from evenly ground chicken breast meat; the mass-volume ratio (m / v, g / mL) of chicken meat and methanol analytical pure A.R is 1:3, extracted 3 times, each extraction time is 0.75h to obtain anabolic hormone extract; evaporate the extract to dryness in a 48°C water bath with a rotary evaporator, and dissolve the residue with a certain amount of 35% (v / v) methanol aqueous solution;

[0022] Purify: C 18 The solid-phase extraction column was activated with 3mL methanol and 3mL water, and the sample solution was passed through the C at a speed of 3mL / min. 18 Column, a...

Embodiment 3

[0024] Embodiment 3: A liquid chromatography method of the present invention for simultaneously detecting 15 kinds of anabolic hormone drug residues in food of animal origin, adopts the following steps:

[0025] Sampling: Mince the pork leg meat after removing fat and other connective tissues, and grind it into a homogenous slurry with a food grinder;

[0026] Extraction: Ultrasonic extraction of anabolic hormones from pork leg meat after uniform grinding; the mass-volume ratio (m / v, g / mL) of pork leg meat to methanol analytically pure A.R is 1:4, extracted 4 times, each extraction time 0.25h to obtain the anabolic hormone extract; evaporate the extract to dryness in a 55°C water bath with a rotary evaporator, and dissolve the residue with a certain amount of 50% (v / v) methanol aqueous solution;

[0027] Purify: C 18 After the solid-phase extraction column was activated with 3mL methanol and 5mL water, the sample solution was passed through the C at a speed of 2.5mL / min. 18 ...

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Abstract

The invention relates to a liquid chromatography for detecting anabolic hormone drug residue in animal-derived food, which is characterized by first sampling: animal musculature is taken off the fat and connective tissue, then minced and evenly ground, and the sample is weighed; extracting: anabolic hormone extract is extracted from the weighed sample by methanol ultrasonic extraction, the extract is evaporated to dryness in water bath through a rotary evaporator, and the residue is dissolved by methanol aqueous solution; purifying: C18 Solidoid extraction column on the extract is carried out solid phase extraction; and testing by devices: the filtrate is tested by opposite phase high efficiency liquid chromatography, quantified by external reference method-peak area, and then carried out with binary gradient elution. In the invention, the detected sample is complex biological sample; the established method can complete one detection in about one hour and is simple and fast, with reliable sensitivity and low cost; the method can analyze more veterinary hormone drug species than the synchronous usage of the existing GC or HPLC methods with easier operation, and has lower cost than the existing GC / MS and LC / MS or LC / MS / MS methods with low solvent toxicity.

Description

technical field [0001] The invention discloses a high-performance liquid chromatography method for simultaneously detecting anabolic hormone drug residues in animal source foods. Background technique [0002] Hormones are a class of trace substances produced in living organisms that regulate the metabolic and physiological functions of the body. Anabolic hormones for veterinary medicine mainly include: steroidal anabolic hormones, non-steroidal estrogens and β 2 - receptor agonists. All three have strong protein assimilation, can increase protein deposition, reduce fat ratio, and thus improve feed conversion rate. However, long-term intake of such hormonal drugs can lead to endocrine disorders and precocious puberty in consumers, increasing the risk of cancer and teratogenicity. As early as January 1, 1988, the European Union issued a ban on the use of growth-promoting anabolic hormones. The Ministry of Agriculture of my country also issued the "Regulations for the Impleme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/89G01N1/28G01N1/34
Inventor 王洪新戴军许婷婷马朝阳吕文平
Owner 上海国矗生物科技有限公司
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