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Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card

A technology of colloidal gold and carbendazim, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, expensive equipment, and difficult to popularize, and achieve the effect of accurate results and simple methods.

Inactive Publication Date: 2016-05-11
NANJING YITE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing technology mainly uses gas chromatography-mass spectrometry, high performance liquid chromatography and other detection methods for the detection of carbendazim, but the defects of these technologies are obvious: expensive equipment, complicated operation, difficult to popularize

Method used

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  • Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card
  • Immune colloidal gold detecting card of carbendazin and preparation method of immune colloidal gold detecting card

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Experimental program
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Effect test

Embodiment 1

[0020] figure 1 , figure 2 Shown embodiment: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold binding pad, and the colloidal gold binding pad is coated with a monoclonal antibody colloidal gold marker; 10 is a coating film, i.e. nitrocellulose Plain membrane, the nitrocellulose membrane is coated with carbendazim-BSA and goat anti-mouse IgG; 11 is a water-absorbent pad, made of water-absorbent materials such as filter paper.

[0021] On one end (sample end) of the PVC rubber plate 9, the sample pad 7 and the bonding pad 8 are adhered, and the sample pad 7 and the bonding pad 8 are in a side-by-side structure.

[0022] A nitrocellulose membrane 10 is adhered in the middle of the PVC rubber sheet 9 . Goat anti-mouse IgG quality control line 3 and carbendazim-BSA detection line 2 are set on the nitrocellulose membrane 10 .

[0023] At the other end of the PVC rubber sheet 9, a water-absorbing pad 11 is adhered. One end of the nitrocellulose ...

Embodiment 2

[0025] The preparation of the carbendazim colloidal gold detection card is realized by the following steps:

[0026] Preparation of colloidal gold solution: Take a 1L Erlenmeyer flask, add 495mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 (0) 5mL, be prepared into 500mL0.01% chloroauric acid aqueous solution, add 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) solution 5-7mL, continue to stir and heat, when the color of the solution turns into transparent purple red completely, stop heating after maintaining 5min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0027] Antibody pretreatment: Centrifuge the carbendazim antibody to be labeled at 1000r / min, 4°C for 20min, take the supernatant, and dilute to 1mg / mL with 0.01mol / LPBS; or dilute to 1mg / mL with 0.01mol / LPBS ml, pass through a 0.22μm filter membrane;

[0028] Preparation of colloidal gold markers: get 40 mL of colloidal gold solution in step ...

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Abstract

The invention discloses an immune colloidal gold detecting card of carbendazin and a preparation method of the immune colloidal gold detecting card, and relates to the technical field of animal source food veterinary drug residue detection. A test strip in a shell of the detecting card is composed of a PVC glue plate, a sample cushion, a colloidal gold conjugate pad, a coating film and a water absorbing cushion, wherein a colloidal gold film is a glass cellulose membrane containing carbendazin monoclonal antibodies, the coating film is a nitrocellulose film and is provided with a line T and a line C, the line T is wrapped by carbendazin protein conjugates, and the line C is wrapped by goat-anti-mouse IgG antibodies. The detecting card can be effectively used for rapidly detecting carbendazin and is convenient to use, rapid and accurate in result.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection in cereal foods, in particular to an immune colloidal gold detection card for carbendazim and a preparation method thereof. Background technique [0002] Carbendazin, chemical name 2-(methoxycarbamoyl)benzimidaxole [2-(methoxy-carbanmoyl)-benzimidaxole], is a high-efficiency and low-toxicity fungicide, which can be used to control various plant diseases . Carbendazim is a commonly used pesticide in the cultivation of asparagus. The export volume of asparagus products in my country is very large, and pesticide residue is a very important restrictive indicator. Therefore, regular detection of carbendazim residues has become a necessary means of monitoring in many countries. The EU stipulates that the maximum residue limit (MRL) of carbendazim in vegetables is 0.1mg / kg. [0003] The existing technologies for the detection of carbendazim mainly use gas chromatography-mass spectr...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531G01N33/532
Inventor 洪霞戴蔚蔚张淑雅
Owner NANJING YITE BIOTECH
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