Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof

A gentamicin and molecular imprinting technology, applied in the field of analytical chemistry, can solve problems such as integration, limiting method specificity, and difficulty in separating mobile phase and mass spectrometry.

Inactive Publication Date: 2005-09-14
HUNAN TONGSEN TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antibiotics have problems such as drug resistance, allergies and stability, and are toxic to humans, so they are an important part of the monitoring of veterinary drug residues in various countries
The method for determining the residual amount of gentamicin, streptomycin and neomycin in animal food mainly adopts ion-pair reversed-phase liquid chromatography at present. This method needs to use ion-pair reagents, and most of these ion-pair reagents are non-volatile Salt is expensive and has a great impact on the life of the chromatographic column. At the same time, it is very difficult to separate the mobile phase and combine it with a mass spectrometer with qualitative capabilities, which limits the specificity of the method; and cannot meet international requirements and standards.

Method used

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  • Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof
  • Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Preparation of Molecularly Imprinted Monolithic Separation Columns for Gentamicin, Streptomycin and Neomycin:

[0018] Add gentamicin, streptomycin, neomycin 0.060, 0.076, 0.078g, methacrylic acid 0.16ml to a 10ml test tube with a thin neckband, mix organic solvent with 9ml (water 1.5ml, methanol 1ml, acetonitrile 6ml, 0.5ml of tetrahydrofuran) were dissolved, 2.4ml of ethylene glycol dimethacrylate was added for vacuum degassing for 15min, then 19.6mg of azobisisobutyronitrile was ultrasonically oscillated for 1min, sealed, and stored at low temperature.

[0019] The above solution was sucked into the capillary, and the two ends of the small column were sealed and placed in a water bath at 60°C for 24 hours of polymerization. The polymer column obtained above was first washed with water and then eluted with methanol. The interior of the filler polymer in the monolithic column was observed by scanning electron microscopy, and the porosity of the molecularly imprinted p...

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Abstract

The invention discloses a molecular engram integral cylinder and its process method for detecting gentamicin, streptomycin and neomycin residue in animal food.The method adopts gentamicin, streptomycin and neomycin as molecular modular and combining in original position to process gentamicin, streptomycin and neomycin molecular engram integral cylinder. The stuff in cylinder is a crosslinked polymers of determined range functional group with fixed hole size and shape, which has memory function for modular molecular spatial structure of gentamicin, streptomycin and neomycin.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a molecularly imprinted monolithic column and a preparation method thereof for highly selective separation applied to the determination of residual amounts of gentamicin, streptomycin and neomycin in animal foods. Background technique [0002] Aminoglycoside antibiotics are a class of broad-spectrum antibacterial drugs that are widely used and require therapeutic drug monitoring, mainly including gentamicin (GM), streptomycin (SM), kanamycin (KM), paromomycin (PM), neomycin (NM), etc. The basic structures of these antibiotics are glycosides formed by the condensation of aminocyclic alcohols and amino sugars, containing amino groups or other basic groups. Antibiotics are widely used in veterinary medicine and animal husbandry production, and most antibiotics are used as veterinary drug additives to prevent animal diseases, reduce mortality and promote g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/00B01D53/00G01N30/08
Inventor 陈波马金余刘程
Owner HUNAN TONGSEN TECH
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