Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof

Abstract

The invention discloses a molecular engram integral cylinder and its process method for detecting gentamicin, streptomycin and neomycin residue in animal food.The method adopts gentamicin, streptomycin and neomycin as molecular modular and combining in original position to process gentamicin, streptomycin and neomycin molecular engram integral cylinder. The stuff in cylinder is a crosslinked polymers of determined range functional group with fixed hole size and shape, which has memory function for modular molecular spatial structure of gentamicin, streptomycin and neomycin.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a molecularly imprinted monolithic column and a preparation method thereof for highly selective separation applied to the determination of residual amounts of gentamicin, streptomycin and neomycin in animal foods. Background technique [0002] Aminoglycoside antibiotics are a class of broad-spectrum antibacterial drugs that are widely used and require therapeutic drug monitoring, mainly including gentamicin (GM), streptomycin (SM), kanamycin (KM), paromomycin (PM), neomycin (NM), etc. The basic structures of these antibiotics are glycosides formed by the condensation of aminocyclic alcohols and amino sugars, containing amino groups or other basic groups. Antibiotics are widely used in veterinary medicine and animal husbandry production, and most antibiotics are used as veterinary drug additives to prevent animal diseases, reduce mortality and promot...

AI Technical Summary

Problems solved by technology

However, antibiotics have problems such as drug resistance, allergies and stability, and are toxic to humans, so they are an important part of the monitoring of veterinary drug residues in various countries

The method for determining the residual amount of gentamicin, streptomycin and neomycin in animal food mainly adopts ion-pair reversed-phase liquid chromatography at present. This method needs to use ion-pair reagents, and most of these ion-pair reagents are non-volatile Salt is expensive and has a great impact on the life of the chromatographic column. At the same time, it is very difficult to separate the mobile phase and combine it with a mass spectrometer with qualitative capabilities, which limits the specificity of the method; and cannot meet international requirements and standards.

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