Animal source food residual medicine detection method

A detection method and technology for residual drugs, applied in the detection field, can solve the problems of single application scope of detection methods, and achieve the effects of high sensitivity, good reproducibility and short time consumption.

Active Publication Date: 2018-02-06
广东省药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sample pretreatment steps in the reported detection method are highly targeted, resulting in a single application range of the detection method

Method used

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  • Animal source food residual medicine detection method
  • Animal source food residual medicine detection method
  • Animal source food residual medicine detection method

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Experimental program
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Embodiment

[0018] 1. Preparation of standard solution

[0019] Trenbolone, Mandrosterone, Nandrolone, Testosterone, Mesterolone, Progesterone, 17-α-Hydroxyprogesterone, 21-α-Hydroxyprogesterone, Fluoxymesterone, Prednisolone, Hydrocortisone, Acetate Oxycorticosterone, Flumetholone, Dexamethasone, Prednisone Acetate, Prednisolone Acetate, Beclomethasone, Dexamethasone Acetate, Fluticasone Propionate, Betamethasone Propionate, Boldenone, Myandrolone, Levonorgestrel, Medroxyprogesterone, Prednisone, Hydroxyprogesterone Acetate, Methylprednisolone, Cortisone Acetate, Hydrocortisone Acetate, Chlormadinone Acetate, Fluoride Acetate Progesterone, fluoromethasone acetate, fludrocetide, betamethasone valerate, megestrol acetate, methyltestosterone, aclomethasone propionate, danazol, etc. were purchased from Dr. Ehrenstor, Germany. Allylgestrol, dydrogesterone, melengestrol acetate, methylprednisolone acetate, clobetasone butyrate, diflurasone diacetate, mifepristone, betamethasone acetate Ester...

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Abstract

The invention discloses an animal source food residual medicine detection method, which comprises the following steps of performing pretreatment on a sample by a QuEChERS method; performing gas chromatgraphy-mass spectrometry combined use detection, wherein according to the method of performing pretreatment on the sample by the QuEChERS method, a test sample is weighed and is put into a centrifugal pipe; ethyl acetate is added; vortex uniform mixing and centrifugation are performed; an organic layer is transferred into another centrifugal pipe; then, a ethyl acetate and ceramic homogeneous proton is added; manual oscillation and centrifugation are performed; supernates in the two times are merged; constant-temperature nitrogen blowing concentration is performed until the materials are nearly dry; acetonitrile is added for re-dissolution; vortex uniform mixing is performed; a QuEChERS purifying agent is added; vortex uniform mixing is performed; the materials pass through a filtering film and wait for machine entering testing. The method has the characteristics that the use operation is simple; the time consumption is short; the sensitivity is high; the repeatability is good. The method is suitable for large-scale application.

Description

technical field [0001] The invention relates to the detection field, in particular to the detection of animal source food by QuEChERS-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Hormones mainly include estrogens (Estrogens), androgens (Androgens), progestogens (Progestogens) and glucocorticoids (Glucocorticoids), which are a class of endocrine disrupting drugs with biological activity. Hormone drugs have been illegally abused in animal husbandry for decades because they can improve feed conversion rate and promote growth, improve breeding efficiency and promote economic growth. Irregular or excessive use of hormonal drugs is the main way leading to hormonal drug residues in animal-derived foods. Many evidences show that hormone drugs residues in animal-derived foods have potential toxicity and carcinogenic hazards to human health, such as breast cancer, uterine cancer, and prostate cancer, which may be caused by hormone dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/72G01N30/06G01N30/14
CPCG01N30/06G01N30/14G01N30/72G01N30/89G01N2030/146
Inventor 罗卓雅庄玥刘亚雄
Owner 广东省药品检验所
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