Bacterial strain and method for producing antibiotic avilamycin special for animal

A technology of avilamycin and antibiotics, which is applied in the field of strains for producing animal-specific antibiotic avilamycin, and can solve the problems of many key control points in the fermentation process, difficult to control the proportion of components, and low ability of bacteria to produce hormones. , to achieve the effect of ensuring safety, preventing animal diseases, and no cross-resistance

Active Publication Date: 2010-02-10
山东胜利生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the industrialized production of avelamycin has not been realized in my country, mainly due to the low production capacity of the strain, ma

Method used

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  • Bacterial strain and method for producing antibiotic avilamycin special for animal

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Embodiment 1

[0042] The strain No. 104 adapted to the high-sugar medium was obtained by continuous ultraviolet mutagenesis of the wild strain SDSL1, and the operation steps were as follows:

[0043] Insert No. 1 strain into the slant medium, and after culturing for 8 days, select a well-grown test tube slant, add 10ml sterile water, wash 2 to 3 times, transfer to a small Erlenmeyer flask with glass beads to disperse and mix, Bacterial suspension, so that the cells are controlled at 10 8 A / ml or so. Put the suspension in a plate and expose it to a 30W ultraviolet light source at a distance of 15 cm for 60 seconds. Serial dilution 10 after irradiation -3 ~10 -5 , coated flat. The culture temperature was 28°C, and the culture time was 9 days. Colonies with good growth were selected for fermentation verification. At the same time, wild bacteria that have not been irradiated by ultraviolet rays are also subjected to fermentation verification. The fermentation medium was prepared with two...

Embodiment 2

[0051] Strain No. 104 was screened for streptomycin resistance to obtain strain No. 232. The 232 strain was mutated by nitrosoguanidine, and the high-yielding strain was screened in combination with streptomycin resistance. The operation steps are as follows:

[0052] Insert the No. 232 strain into the slant medium, and after culturing for 8 days, select a well-grown test tube slant, add 10ml of sterile water, wash 2 to 3 times, transfer to a small Erlenmeyer flask with glass beads to disperse and mix, Bacterial suspension, so that the cells are controlled at 10 8 A / ml or so.

[0053] In a fume hood, weigh 20 mg of nitrosoguanidine, add acetone at a ratio of 0.1 ml / mg to dissolve it, and then dilute it with 4 times citrate buffer, so that the concentration of the solution is 2 mg / ml. The spore suspension and the nitrosoguanidine solution were mixed at a ratio of 1:2, and acted at 26-28°C for 30 minutes. After the treatment, centrifuge, wash and centrifuge more than 10 time...

Embodiment 3

[0062] The No. 1009 strain that had been screened by mutagenesis was purified naturally through several rounds. The spore suspension used is prepared with example 1, and concentration is about 10 8 pieces / ml. The operation is as follows:

[0063] Select 8 thick test tubes, add 9 ml of sterile water to No. 1-8, draw 1 ml of spore suspension into No. 1 tube, shake and mix well, suck 1 ml from No. 1 tube to No. 2 tube, and so on, to make Gradient concentration. Draw 0.05ml from No. 6 tube and smear evenly in the plate culture medium. In the same way, draw 0.1 and 0.2ml of the dilution solution from tubes 7 and 8, respectively, and put them in the plate culture medium. The culture temperature was 28°C, and the culture time was 8 days. Select a single colony that is robust and free of bacteria and insert it into the slant culture, pass the seed culture, and then carry out fermentation verification.

[0064] (1) Plate medium: cornstarch 25g / L, KNO 3 1g / L, K 2 HPO 4 0.5g / L, ...

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Abstract

The invention belongs to the technical field of biological pharmacy, in particular to a bacterial strain green chromogenic streptomyces viridochromogenes SDSL1119 for producing antibiotic avilamycin special for an animal and a method for producing the avilamycin by the bacterial strain. The bacterial strain is collected in a CGMCC on July 21st, 2009 with the collection number of CGMCCNo.3198. Themethod comprises the steps of oblique plane culture, seed culture and fermentation culture. The invention has the advantages of environmental-friendly production process, high product bioactivity, favorable antibiosis effect and no residue and cross medicine resistance and can effectively prevent animal diseases and ensure the safety of animal source foods.

Description

technical field [0001] The invention relates to the field of microorganisms and their fermented production compounds, belonging to the technical field of biopharmaceuticals, in particular to a bacterial strain and a method for producing the animal-specific antibiotic avelamycin. Background technique [0002] The intensive and high-density breeding methods of modern animal husbandry have brought about the improvement of production efficiency, but also greatly increased the risk of animal disease transmission. As a feed additive, antibiotics can not only promote animal growth, but more importantly, prevent pathogenic bacteria infection and group diseases, which have made a significant contribution to the development of intensive breeding industry and greatly improved the economic benefits of the breeding industry. But at the same time, problems such as drug-resistant strains and drug residues have also arisen. Due to the lack of animal-specific antibiotics in my country, a la...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/60A23K1/17C12R1/465A23K20/195
Inventor 雷宪军孔凡亭胡江林关志勇
Owner 山东胜利生物工程有限公司
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