Spectinomycin colloidal gold detection card

A spectinomycin and colloidal gold technology, which is used in measurement devices, biological tests, material inspection products, etc., can solve problems such as detection cards without spectinomycin, and achieve the effects of saving detection costs, rapid detection, and high sensitivity

Inactive Publication Date: 2014-05-21
JIANGSU WISE SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing technology mainly relies on chromatography and mass spectrometry for the detection of spectinomycin, and there are also enzyme-linked immunoassay kits, but no spectinomycin detection card has yet appeared.

Method used

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  • Spectinomycin colloidal gold detection card
  • Spectinomycin colloidal gold detection card
  • Spectinomycin colloidal gold detection card

Examples

Experimental program
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Embodiment Construction

[0012] 1. Preparation of Labeled Colloidal Gold

[0013] Preparation of colloidal gold solution: 1 g chloroauric acid (HAuCl 4 ) powder was dissolved in 100 mL of ultrapure water to prepare a 1% chloroauric acid solution; then take 1 L of ultrapure water and heat it on a special electric furnace, add 10 mL of 1% trisodium citrate aqueous solution after boiling, and continue Heat, add 10 mL of 1% chloroauric acid solution after 5 minutes, heat again to boiling, wait for the color to turn into wine red, start timing, stop heating after 10 minutes, take it off, cool naturally, and set the volume to 1 L, that is It is a colloidal gold solution;

[0014] Antibody labeling: 0.1 mol / l K required for determination of antibody 2 CO 3 ratio, take 100 mL of colloidal gold solution, add this ratio of 0.1 mol / L K 2 CO 3 , mix well (about 5 min), then dissolve spectinomycin and the second species of animal protein with PBS (0.01 mol / L, pH7.4) to dissolve and dilute to 2 mg / mL, add 2 mL...

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Abstract

The invention relates to a spectinomycin colloidal gold detection card. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen 1 bar which is applied to detection and is used as a detection line, and at the same time is coated by an IgG1 bar which resists the second species of animal protein and is used as a reference line. The rapid detection test paper bar has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 40 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal source food to carry out rapid detection on spectinomycin residues. The spectinomycin colloidal gold detection card provided by the invention has the advantages of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi quantitative detection.

Description

technical field [0001] The invention belongs to the field of food safety testing, and specifically relates to a detection method of harmful residues in food, especially a detection method of spectinomycin test paper. Background technique [0002] Spectinomycin (English name spectinomycine), also known as spectinomycin, neresin, spectinomycin, actinomycin spectinomycin, spectinomycin. Belonging to aminoglycoside antibiotics, it is effective against Gram-negative bacteria (such as Pseudomonas aeruginosa, Proteus) and Staphylococcus aureus, especially against Neisseria gonorrhoeae. Spectinomycin is an aminocycloalcohol compound isolated from Streptomyces spectabilis, which mainly exists in the tissues and livers of animal products, can damage the eighth pair of cranial nerves, and has renal toxicity and neuromuscular inhibition. break effect. [0003] In 2002, the European Union promulgated the maximum residue limit of spectinomycin in tissues (residue markers are all in thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/558
Inventor 杜道林张勇
Owner JIANGSU WISE SCI & TECH DEV
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