Immune colloidal gold detect card of chlorpromazine, and preparation method thereof

Abstract

The invention discloses an immune colloidal gold detect card of chlorpromazine, and a preparation method thereof, and belongs to the technical field of animal-derived food veterinary drug residue detection. A test strip of an outer housing of the immune colloidal gold detect card is composed of a PVC slab, a sample pad, a colloidal gold conjugate pad, a coating membrane, and an absorbent pad; a colloidal gold membrane is a glass cellulose membrane containing chlorpromazine monoclonal antibodies; the coating membrane is a nitrocellulose memebrane, and is provided with a T line and a C line; the T line contains chlorpromazine protein conjugates via coating; and the C line contains goat anti-mouse IgG antibodies via coating. The immune colloidal gold detect card is used for rapid detection of chlorpromazine effectively, and is convenient and rapid for using; and detection results are accurate.

Description

technical field The invention relates to the technical field of animal-derived food veterinary drug residue detection, in particular to an immune colloidal gold detection card for chlorpromazine and a preparation method thereof. Background technique Chlorpromazine is a phenothiazine metabolite. This drug is mainly metabolized in the liver and is prone to drug residues. Residual chlorpromazine and some metabolites with the original drug activity can cause leukopenia and agranulocytosis, thereby causing Human liver, kidney disease, but also can cause eye complications. In some countries and regions, breeders add chlorpromazine to the feeding of edible animals, resulting in chlorpromazine residues in related animal foods. Therefore, regular detection of chlorpromazine residues has become a necessary means of monitoring in many countries. . The detection methods for chlorpromazine in the prior art are mainly high performance liquid chromatography-tandem mass spectrometry, hig...

AI Technical Summary

Problems solved by technology

Existing technologies are mainly detection methods such as high performance liquid chromatography-tandem mass spectrometry and high performance liquid chromatography for the detection of chlorpromazine, but the defects of these technologies are obvious: the equipment is expensive, the operation is complicated, and it is difficult to popularize

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