Method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously

A technology for compound salvia miltiorrhiza and extracts, which can be used in material separation, measuring devices, analyzing materials, etc., and can solve problems such as inconvenient mass balance.

Active Publication Date: 2018-05-04
TIANJIN TASLY PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application of multiple analytical methods has brought great inconvenience to the study of mass balance

Method used

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  • Method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously
  • Method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously
  • Method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: detection method

[0088] 1. Preparation of standard solution and internal standard solution

[0089] 1.1 Preparation of standard series solutions

[0090] Weigh an appropriate amount of the standard substance to be tested, weigh it accurately, add 70% methanol water to make a solution with a concentration of 500 μg / mL, and use it as the stock solution of each sample to be tested. Accurately measure the appropriate amount of each stock solution of the analyte, and use 70% methanol water to prepare tetrasaccharide (concentration is about 70000ng / mL), sucrose (concentration is about 50000ng / mL), salvianolic acid B (concentration is about 12000ng / mL) mL), ginsenoside Rg 1 (concentration is about 8000ng / mL), glucose (concentration is about 6000ng / mL), trisaccharide, ginsenoside Rb 1 , Ginsenoside Rd (concentration is about 4000ng / mL), pyroglutamic acid, rosmarinic acid, notoginsenoside R 1 (concentration of about 2000ng / mL) and the rest of the analyte (con...

Embodiment 2

[0105] The assay of embodiment 2 31 kinds of compositions

[0106] 1. Preparation of sample solution for test

[0107] Compound Danshen extracts S01-S05 (batch numbers 20131205, 20141103, 20120201, 20150310 and 20150310) were provided by Tasly Pharmaceutical Group Co., Ltd.; Danshen medicinal materials S06-S27 were collected from several provinces in China, and Panax notoginseng medicinal materials S28-S52 were collected from Yunnan Province, and crushed at the Tasly Research Institute.

[0108] Take 0.10 g of compound Danshen extract, Danshen extract, Panax notoginseng extract, Danshen powder and Panax notoginseng powder, weigh them accurately, add 10 mL of 70% methanol, sonicate for 30 min, centrifuge for 10 min (16,000 rpm), filter through 0.22 μm Membrane, respectively accurately measure the same amount of sample solution and IS reference solution, and mix them to prepare the sample solution containing internal standard for RP-HILIC-MS / MS analysis. All solutions were sto...

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Abstract

The invention relates to a method for measuring 31 components in compound radix salviae miltiorrhizae extract or related medicinal materials simultaneously. The method adopts a direct series-reverse phase-hydrophilic interaction chromatography mass spectrometer combination method and comprises steps as follows: step one, preparing a sample solution; step two, preparing series of standard solutions; step three, preparing an internal standard solution; step four, preparing a solution for test products; step five, injecting the solution obtained in the step four into a direct series-reverse phase-hydrophilic interaction liquid chromatography mass spectrometer combination system so as to obtain an extracted ion flow diagram of 31 analytes, and calculating content of the 31 components in the compound radix salviae miltiorrhizae extract or the related medicinal materials according to the extracted ion flow diagram.

Description

Technical field: [0001] The invention relates to a method for detecting effective components of traditional Chinese medicine, in particular to a method for simultaneously determining 31 components in compound salvia miltiorrhiza extract or related medicinal materials. Background technique: [0002] The study of mass balance is of great significance for elucidating the composition of the chemical substance groups of traditional Chinese medicines. The chemical composition of traditional Chinese medicine is extremely complex, containing many primary metabolites and secondary metabolites. [0003] Preliminary studies have shown that compound Danshen dripping pills (made from Danshen, Panax notoginseng, and borneol, see the 2015 edition of the Chinese Pharmacopoeia for specific preparation methods) mainly contain oligosaccharides, amino acids, nucleosides, organic acids, phenolic acids, saponins, and tanshinones compound. Due to the large differences in the structure and physic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/89
CPCG01N30/02G01N30/89
Inventor 佟玲李云飞李东翔孙万阳芦勤玮
Owner TIANJIN TASLY PHARMA CO LTD
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