Black ginseng extract, black ginseng quick-release pellets and application thereof for treating renal interstitial fibrosis

A technology of renal interstitial fibrosis and black ginseng, which is applied in the field of medicine, can solve the problems of insufficient extraction of saponins, lack of concentration gradient in solution, long extraction and separation time, etc., and achieve stable process, feasible method and reliable results

Active Publication Date: 2021-11-30
YANBIAN UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The extraction method of black ginseng is in the local standard black ginseng of Jilin Province (DB 22 / T 2758-2017), and the preparation method of the test solution under the determination method of ginseng content in the Chinese Pharmacopoeia is adopted. This method takes a long time for extraction and separation. There are many steps and the concentration gradient of the target components inside and outside the matrix is ​​relatively large, so the extraction of saponins is not thorough enough
The reflux extraction method commonly used in traditional Chinese medicine uses the solution to be in a boiling state to strengthen the disturbance between the solution and the matrix and reduce the diffusion resistance of the fluid film on the surface of the matrix. However, this method is not conducive to the further dissolution of components due to the lack of a concentration gradient in the solution at a certain stage of extraction. , when using this method to extract black ginseng, the total content of rare ginsenosides Rg3, Rg5 and Rk1 is low

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  • Black ginseng extract, black ginseng quick-release pellets and application thereof for treating renal interstitial fibrosis
  • Black ginseng extract, black ginseng quick-release pellets and application thereof for treating renal interstitial fibrosis
  • Black ginseng extract, black ginseng quick-release pellets and application thereof for treating renal interstitial fibrosis

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preparation example Construction

[0032] The invention provides a preparation method of black ginseng extract, comprising the following steps:

[0033] Mix black ginseng powder with ethanol water solution with a volume fraction of 50%-70%, ultrasonically extract at 40-80KHz for 20-50min, extract 3-4 times, combine the extracts and filter to obtain the filtrate, concentrate and freeze-dry to obtain black ginseng extract ; The mass ratio of the black ginseng powder to the ethanol aqueous solution is 1g: (10-20) mL.

[0034] In the present invention, the volume fraction of the ethanol aqueous solution is preferably 55% to 65%; the mass ratio of the black ginseng powder to the ethanol aqueous solution is preferably 1g: (15 to 20) mL; the frequency of the ultrasound is preferably It is 60~80KHZ; The time of single ultrasonic is preferably 35~48min; More preferably the volume fraction of described ethanol aqueous solution is 65%, the mass ratio of the quality of described black ginseng powder and ethanol aqueous sol...

Embodiment 1

[0059] Black ginseng extract sample preparation method

[0060] Accurately weigh and dry 4-year-old black ginseng powder, use 65% ethanol as the solvent, and the ratio of solid to liquid is 1:20, use ultrasonic extraction method, extract 3 times, each ultrasonic 48min, ultrasonic frequency 80KHz, combine 3 extracts, Filter, concentrate to 1mL equivalent to 1g black ginseng decoction pieces, and freeze-dry to obtain the black ginseng extract sample.

Embodiment 2

[0062] Black ginseng extract sample preparation method

[0063] Precisely weigh and dry 4-year-old bearded black ginseng powder, use 65% ethanol as solvent, the ratio of solid to liquid is 1:20, use ultrasonic extraction method, extract 3 times, each time ultrasonic 48min, ultrasonic frequency 80KHz, combine 3 extracts , filtered, concentrated to a concentration of 1 mL equivalent to 1 g of black ginseng decoction pieces, and freeze-dried to obtain a black ginseng extract sample.

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Abstract

The invention provides a black ginseng extract for treating renal interstitial fibrosis, black ginseng quick-release pellets and applications thereof, which belong to the technical field of medicines. Black ginseng extract Ginsenosides Rg3, Rg5 and Rk1 are efficiently extracted from black ginseng by ultrasonic extraction. The present invention also provides black ginseng quick-release pellets, which include the following components in mass percent: 15%-35% of the black ginseng extract, 25%-47% of microcrystalline cellulose, and 23%-26% of lactose , 5% to 8% of croscarmellose sodium starch, 5% to 8% of low-substituted hydroxypropyl cellulose and 50% to 65% ethanol aqueous solution by volume fraction of the balance. The pharmacological effect and pharmacological mechanism of intervening renal interstitial fibrosis were investigated through in vivo and in vitro experiments, indicating that black ginseng extract or black ginseng quick-release pellets have the potential to prepare drugs for treating renal interstitial fibrosis.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a black ginseng extract for treating renal interstitial fibrosis, black ginseng quick-release pellets and applications thereof. Background technique [0002] Chronic kidney disease (CKD) is a serious clinical problem. Currently, renal fibrosis, especially renal interstitial fibrosis (Renal interstitial fibrosis, RIF) is recognized as a common disease in end-stage renal disease (ESRD). Pathway, in which the development of renal fibrosis is significantly related to the occurrence of CKD. RIF is a progressive process of constant changes, characterized by the continuous generation of activated myofibroblasts (myofibroblast) and excess extracellular matrix (Extracellular matrix, ECM) in the renal tubules, and activated myofibroblasts are considered to be the pathogenesis of RIF main factor. The origin of activated myofibroblasts is still unclear, and there is evidence ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/20A61K36/258A61P13/12A61K125/00
CPCA61K9/2059A61K36/258A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61P13/12
Inventor 郭建鹏陈林崔福顺李官浩左阿龙蒋世翠
Owner YANBIAN UNIV
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