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A callus, subculture technology, applied in the field of plant tissue culture research, can solve the problems of tissue fragmentation, weight loss, weight gain, etc., and achieve the effect of strong water absorption
Inactive Publication Date: 2013-02-06
胡能兵
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The callus grows on the sticky and soft medium, the base is intertwined with the medium, and its own tissue is very fragile. During the sampling process, it is easy to cause the tissue to break, resulting in weight loss, and it is easy to be damaged by the base. Carried residual medium, resulting in weight gain
The existence of these conditions makes the weight gain effect of callus subculture "unpredictable"
Method used
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Embodiment 1
[0018] Embodiment 1: Determination of weight gain in subculture of okra okra callus:
[0019] 1. Pad filter paper: First, put a piece of sterilized filter paper on the sterilized and solidified medium, weigh it, and record it as G a 。
[0020] 2. Inoculation and weighing: Inoculate the callus of okra okra to be subcultured into pieces on the filter paper, weigh it, and record it as G b ，G b with G a The difference is the initial weight of the callus.
[0021] 3. Cultivation: Cover the bottle cap of the tissue culture bottle and place it in the tissue culture room for 15 days.
[0022] 4. Sampling and weighing: After the culture was completed, the callus on the filter paper was taken out from the tissue culture bottle with tweezers and weighed, and recorded as G c 。
[0023] 5. Calculation of callus growth weight gain (G) during subculture:
[0024] G=G c -G b -G a
Embodiment 2
[0025] Embodiment 2: rice callus subculture weight gain measurement:
[0026] 1. Pad filter paper: First, put a piece of sterilized filter paper on the sterilized and solidified medium, weigh it, and record it as G a 。
[0027] 2. Inoculation and weighing: Cut the rice callus to be subcultured and inoculate it on the filter paper, weigh it, and record it as G b ，G b with G a The difference is the initial weight of the callus.
[0028] 3. Cultivation: Cover the bottle cap of the tissue culture bottle and place it in the tissue culture room for 18 days.
[0029] 4. Sampling and weighing: After the culture was completed, the callus on the filter paper was taken out from the tissue culture bottle with tweezers and weighed, and recorded as G c 。
[0030] 5. Calculation of callus growth weight gain (G) during subculture:
[0031] G=G c -G b -G a
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Abstract
The invention discloses a simple plant callus subculture weight increase determination method during plant tissue culture. The method includes: firstly, placing a piece of disinfected filter paper on a solidified culture medium, weighing, and dicing a to-be-subcultured callus and inoculating the diced callus on the filter paper prior to weighing, wherein the difference between two-time weighing results serves as initial weight; and after culture, sampling and weighing the grown callus, wherein a numerical value obtained by subtracting the initial weight from grown weight serves as increased weight during subculture. The culture medium supplies water and nutrients to the callus by the aid of a filter paper bridge, the callus is in non-sticky connection with the filter paper, is unattached to the culture medium, can be tweezed directly by tweezers and is less prone to crushing, and the method is simple, convenient, feasible, free of interference during weighing, accurate and reliable.
Description
technical field [0001] The invention belongs to a plant tissue culture research method, in particular to a measurement technique for accurately measuring the growth increment of callus subculture. Background technique [0002] Callus subculture is an important part of plant tissue culture. In related research work, in order to compare the technical effects of different treatments on callus, it is often necessary to compare the weight of callus before and after subculture. The callus grows on the sticky and soft medium, the base is intertwined with the medium, and its own tissue is very fragile. During the sampling process, it is easy to cause the tissue to break, resulting in weight loss, and it is easy to be damaged by the base. Carried residual medium, resulting in weight gain. The existence of these conditions makes the weight gain effect of callus subculture "unpredictable". This puzzle remains unresolved. The purpose of the present invention is to create a simple, a...
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