Ergothioneine detection method

A technology for ergothioneine and its detection method, which is applied in the technical fields of analytical chemistry and biochemistry, can solve problems such as the lack of standards for ergothioneine detection methods, achieve ideal retention and elution effects, strong versatility, and stable detection conditions Effect
CN104749263AActive Publication Date: 2015-07-01TIANJIN SINONOCY BIOLOGICAL TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN SINONOCY BIOLOGICAL TECH CO LTD
Publication Date
2015-07-01

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Abstract

The invention relates to a high pressure liquid chromatography detection method for ergothioneine. According to the invention, an hydrophilic interaction chromatography (HILIC) analysis chromatographic column is employed for performing a high pressure liquid chromatography analysis on a sample containing ergothioneine, the chromatographic column employs a HILIC filler as a fixed phase, a solution of acetonitrile and water (v / v) with ratio of (80-95): (20-5) is taken as a mobile phase, the pH value of the solution is 5.6-8.0, and the column temperature is 25-45 DEG C. the invention also relates to a quantitative determination method of ergothioneine.
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Description

technical field

[0001] The invention belongs to the fields of biochemical technology and analytical chemistry, and relates to a method for determining ergothioneine and its content. Background technique

[0002] Ergothioneine (L-Ergothioneine, EGT) is a rare natural chiral amino acid strong antioxidant, a unique cell physiological protection agent, with anti-oxidation, chelating metal ions, preventing ultraviolet radiation and regulating biological energy , gene expression, immune system and many other functions. Due to the special biological functions and pharmacological activities of ergothioneine, such as: anti-inflammation, defense against nerve tissue degeneration, prevention of copper-induced oxidative damage to DNA and proteins, inhibition of developmental defects, protection of the liver, etc., and humans and animals will not It is generally recognized as safe (GRAS) products without any toxic side effects. Therefore, ergothioneine has broad application prospects in...

Claims

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