Online hydrophilic interaction chromatography/reversed phase chromatography serial interface device and application thereof

Abstract

The invention relates to the technical field of liquid chromatography, in particular to an online hydrophilic interaction chromatography/reversed phase chromatography serial interface device. The online hydrophilic interaction chromatography/reversed phase chromatography serial interface device comprises a high-pressure inert gas source and relevant pressure reduction and valve switching devices. Specifically, one end of a first-dimensional chromatographic column is connected with an inert gas (such as nitrogen) pressure reducing valve and a pump system through a switching valve and pipelines, and the other end of the first-dimensional chromatographic column is connected with a second-dimensional chromatographic column and waste liquid through a switching valve and pipelines; by switching the valves, the high-pressure inert gas replaces a solvent left in the first-dimensional chromatographic column, the problem of incompatibility of solvents in online series connection is solved, reservation of samples on the second-dimensional chromatographic column is guaranteed, and therefore hydrophilic interaction chromatography and reversed phase chromatography are connected in series. An interface is convenient to use, simple in operation, capable of saving time, efficient and high in universality; due to the fact that the hydrophilic interaction chromatography and the reversed phase chromatography are directly connected in series, the samples can be online, quickly and continuously operated, and losses of the samples are reduced.

Description

technical field [0001] The invention relates to the technical field of liquid chromatography, and specifically relates to an online hydrophilic interaction chromatography / reversed-phase chromatography serial interface device, which realizes the direct mutual connection of online hydrophilic interaction chromatography / reversed-phase chromatography. Background technique [0002] Hydrophilic interaction chromatography (HILIC), as a liquid chromatography mode for separating polar compounds, was first proposed by Alpert in 1990 (Alpert J., J. Chromatogr.A, 1990, 400:177). Its main features are: use of a polar stationary phase similar to normal phase chromatography and a water / organic solvent (usually acetonitrile) mobile phase (where water is the strong eluting solvent). Many studies have shown that when water-water-soluble organic solvents are used as the mobile phase, the surface of the hydrophilic stationary phase will contain a layer of water-rich layer, and the retention of ...

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Problems solved by technology

[0003] However, in practical applications, the organic phase as a strong eluent in RP is a weak eluent of mobile phase in HILIC, while water as a weak eluent in RP is a strong eluent in HILIC, Its content will significantly affect the retention of

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