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Phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use

A metal-organic framework and magnetic metal technology, applied in the field of magnetic metal-organic framework microspheres and their preparation, can solve the problems of small specific surface area and limited functional groups of glycoproteins, achieve large specific surface area, increase adsorption capacity, and improve richness. The effect of gathering ability

Inactive Publication Date: 2018-10-12
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ability of most current materials to enrich glycoproteins is often limited by the small specific surface area and limited functional groups.

Method used

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  • Phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use
  • Phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use
  • Phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Fe 3 o 4 @SiO 2 Synthesis of @UiO-PBA microspheres

[0028] (1) Add 500mg of succinic anhydride (SA) and 1mL of 3-aminopropyltriethoxysilane (APTES) to 30mL of ethanol, ultrasonically mix, stir at 30°C for 3h; add 40mg of Fe to the above solution 3 o 4 @SiO 2 , 20mL ethanol and 3mL deionized water, continue stirring for 12h, magnetically separate, wash with deionized water and ethanol, and vacuum dry to obtain carboxyl functionalized Fe 3 o 4 @SiO 2 (Fe 3 o 4 @SiO 2 -COOH).

[0029] (2) 93.22mg zirconium chloride (ZrCl 4 ) and 72.46mg 2-aminoterephthalic acid (H 2 BDC-NH 2 ) was dispersed in 60 μL acetic acid and 30 mL N,N-dimethylformamide (DMF), and 20 mg of Fe 3 o 4 @SiO 2 -COOH was added to the above solution, reacted at 130°C for 4h, magnetically separated, washed with deionized water and ethanol, and dried at 60°C to obtain MOF-modified magnetic microspheres (Fe 3 o 4 @SiO 2 @UiO-66-NH 2 ).

[0030] (3) Add 200mg 4-carboxyphenylboro...

Embodiment 2

[0031] Example 2: Fe 3 o 4 @SiO 2 @UiO-PBA microspheres for the enrichment of glycoproteins

[0032] Take 5mg Fe 3 o 4 @SiO 2 @UiO-PBA was dissolved in 5mL protein solution with pH=7.4, shaken at room temperature for 30min, magnetically separated Fe bound to glycoprotein 3 o 4 @SiO 2 @UiO-PBA was separated from the solution, and the supernatant was analyzed and detected by HPLC. The separated particles were washed 5 times with PBS solution with pH=2.5 to elute the adsorbed glycoprotein.

Embodiment 3

[0033] Example 3: Egg white was diluted 500 times with PBS buffer solution with pH = 7.4, and then 0.2, 0.5, 0.8 mg / mL ovalbumin (OVA) was added to the diluted egg white respectively to prepare egg white samples. Will Fe 3 o 4 @SiO 2 @UiO-PBA was incubated with the three samples for 30 min respectively, the nanoparticles were collected by magnetic separation, washed 5 times with PBS solution with pH = 2.5, and the supernatant was analyzed by HPLC.

[0034] Using scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction to analyze Fe 3 o 4 @SiO 2 Morphological and structural characterization of @UiO-PBA microspheres. Such as figure 2 Shown Fe 3 o 4 @SiO 2 It is a uniform spherical shape with a particle size of about 380nm; after coating the MOF material, Fe 3 o 4 @SiO 2 @UiO-66-NH 2 The microspheres have an obvious core-shell structure; after grafting phenylboronic acid, Fe3O4@SiO2@UiO-PBA and Fe 3 o 4 @SiO 2 @UiO-66-NH 2 The morpho...

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Abstract

The invention discloses a phenylboronic acid functionalized magnetic metal organic skeleton microsphere and its synthesis method and use for enriching glycoprotein. The microsphere orderly comprises amagnetic core, a connecting layer, a metal organic skeleton layer and a phenylboronic acid outer layer from inside to outside, has a large specific surface area and good hydrophilicity, contains a large number of functional groups of boric acid and can specifically enrich glycoprotein through hydrophilic interaction and boric acid affinity.

Description

technical field [0001] The invention belongs to the field of advanced nanometer materials and biotechnology, and specifically relates to a phenylboronic acid-functionalized magnetic metal-organic framework microsphere capable of separating and enriching glycoproteins and a preparation method thereof. Background technique [0002] Protein glycosylation is one of the most important forms of post-translational modification, which is involved in important life processes such as fertilization, development, metabolism, cell recognition and signal transduction of eukaryotic cells. Studies have shown that protein glycosylation also exists in the occurrence and development of some major diseases, such as autoimmune diseases, neurological diseases and cancer. However, their analytical detection in complex biological samples is particularly difficult due to the inherently low abundance of glycoproteins and signal interference from a large number of non-glycoproteins. Therefore, findin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/30B01J20/28B01J13/06B82Y30/00C07K1/14
CPCB01J13/06B01J20/226B82Y30/00C07K1/14B01J20/28009B01J20/28021
Inventor 陈春燕李淑婷钟冠群
Owner XIANGTAN UNIV
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