6 results about "Dna identification" patented technology

The invention discloses a primer combination, a kit and an identification method for parrot-shaped species identification, and belongs to the technical field of DNA identification. According to the primer combination provided by the invention, the long fragment amplification of the parrot-shaped mitochondrial genome can be realized through specific design, and a complete mitochondrial DNA sequence can be successfully obtained from trace and mixed difficult samples. The obtained full-length genome is rich in information amount, and related species with similar forms can be distinguished with high resolution. The kit provided by the invention can be adapted to a mainstream length reading sequencing platform and is simple to operate. The identification method for parrot-shaped species provided by the invention is efficient and reliable in process, and is especially good at treating old samples, trace samples or samples with complex components which are common in judicial evidence collection or field monitoring. By combining long fragment amplification and long read length sequencing, the finally output species identification result has the parrot-shaped parrot seed level or even subspecies level distinguishing precision, and the accuracy is high.

The invention discloses an agrobacterium tumefaciens-mediated sweet osmanthus callus genetic transformation method which comprises the following steps: (1) collecting sweet osmanthus seeds, disinfecting and sterilizing, inducing zygotic embryos to form calluses, and carrying out multiplication culture; (2) activating and propagating agrobacterium tumefaciens, resuspending the agrobacterium tumefaciens until OD600 is 0.4-0.6 by using a buffer solution, infecting healthy calluses, performing vacuum treatment and co-culture, and performing screening culture; and (3) verifying the integration and expression of the target gene through visual identification, DNA identification, real-time fluorescent quantitative PCR or western blot analysis. According to the method, a stable genetic transformation system of the osmanthus fragrans callus is established for the first time, the hormone proportion, infection conditions and screening parameters are optimized, overexpression of OfPSI1 and RUBY genes is successfully realized, the transformation efficiency is high, and the operation is simple and convenient. The invention provides a reliable technical platform for sweet osmanthus gene function research and molecular breeding, and has important scientific research and application values.

The application provides a traditional Chinese medicinal material plant tracing method and device, a terminal and a storage medium, and relates to the technical field of medicinal material tracing. The method comprises the following steps: determining the plant information of an original plant corresponding to a plant to be analyzed according to a tracing code of the plant to be analyzed, the tracing code being an RFID or NFC tag printed on the package of the plant to be analyzed, and the plant information comprising a traditional Chinese medicinal material planting enterprise, a planting site, a planting time, a species, a variety and a DNA sequence; generating a first fingerprint vector and a second fingerprint vector respectively according to the DNA sequence, the first fingerprint vector being a fingerprint vector of the plant to be analyzed, and the second fingerprint vector being a fingerprint vector of the original plant, the fingerprint vector comprising the encoding values of target bases at multiple candidate sites; calculating a similarity measure according to the first fingerprint vector and the second fingerprint vector, and determining the similarity between the plant to be analyzed and the original plant based on the similarity measure. The application can improve the reliability of traditional Chinese medicinal material tracing and the DNA identification comparison efficiency.

Genetic samples are obtained from separate people, and at least a portion of each are purposefully combined before testing to form a pooled genetic sample. The pooled genetic sample is tested for the presence of a signature for a given known ailment. DNA identification uses discovered InDels in a region of InDel variation in a genetic sample. A pair-wise comparison is performed to reference InDels, and a distance is measured between the first InDel and the reference Indel. Reference kmers are identified in a reference genome, and in a test sample. The plurality of sample kmers are filtered to those which have a 1 edit distance from a corresponding one of the plurality of reference kmers. Reads that have kmers that do not have a 1 edit distance from the corresponding one of the plurality of reference kmers are identified, and multiple single-mutations are eliminated from candidate InDel reads.

The invention relates to the field of molecular biology, in particular to application of an SSR primer pair combination of slash pines to construction of core germplasm and fingerprint maps of slash pines. The nucleotide sequence of the SSR primer pair combination is as shown in SEQ ID NO. 1-40. The SSR primer pair combination is used for amplifying template DNA, then Power Core software is used for obtaining a core germplasm material with the sampling proportion of 10.31%, then the effectiveness of a selected core set is verified through genetic parameter t inspection and principal coordinate analysis, and the verification result shows that the slash pine core germplasm can eliminate genetic redundancy, so that genetic parameters are improved; and finally, successfully constructing a pinus elliottii germplasm resource fingerprint spectrum and a pinus elliottii DNA molecular identity card through the SSR primer pair combination. Therefore, the SSR primer pair combination provided by the invention can be used for constructing a slash pine core germplasm, a slash pine DNA fingerprint spectrum and a slash pine DNA molecular identity card.

The application discloses a method for preparing grape DNA identification codes by DNA sequence variation and application thereof; the application utilizes multiple pairs of SSR primers to perform PCR amplification on grape germplasm resource genomic DNA, clear and polymorphic SSR fingerprint bands are obtained through gel electrophoresis, and information on the presence or absence of bands of different lengths in the amplification products is converted into 0 / 1 binary data. On the basis of gradually screening and combining multiple pairs of SSR primers to realize complete differentiation of varieties, the multiple-bit binary data is converted into a decimal number in a radix conversion mode, and finally, a unique DNA identification code of each sample is generated through a specific conversion method; a high-efficiency technology for genotype identification by using plant DNA sequence variation is formed, the germplasm resource identification capability is significantly improved, and the technology is suitable for scenes such as plant genetic diversity analysis, germplasm authenticity and purity identification, variety specificity identification and the like.