STR gene data analysis method

Abstract

The invention discloses a STR gene data analysis method. On the basis of C + + language, short tandem repeat (STR) gene sequencing data in DNA is subjected to positioning full-automatic analysis, thedata processing efficiency is integrally improved, a gene region associated with specific characters is finally obtained, and a rich positioning result display chart is provided. The algorithm supports analysis of multi-color fluorescence channels, supports the kit commonly used in the market at present, suports an original data file format output by a common sequencer, can automatically identifyand remove miscellaneous peaks generated in inspection, and can automatically calibrate peak pattern dislocation generated in the sequencing process. According to the invention, full-automatic data analysis of a multi-color fluorescence color channel is carried out on a DNA gene short tandem sequence STR in DNA identification and detection by a forensic medicine. The situation that DNA sequencingonly depends on foreign software analysis is broken through, a process is established for developing domestic sequencers and domestic reagents in China, and a domestication development process in thefield of forensic identification and detection of DNA in China is accelerated.

Description

technical field [0001] The invention relates to the technical field of STR gene data, in particular to a method for analyzing STR gene data. Background technique [0002] The short tandem repeat sequence in microsatellite DNA is referred to as STR, which is a kind of DNA polymorphism, which widely exists on 23 pairs of chromosomes in the human genome, usually consisting of 2-6bp repeating units, and the difference between different individuals (or alleles) The difference between them is generally only manifested as the difference in the repeat number of the repeat unit. The STR locus is an important genetic marker. On the locus with a high degree of polymorphism, its individual recognition ability is strong, and it can be detected simply by PCR technology. The STR locus has the following characteristics: the number of STR polymorphic loci is large, the fragment length is generally less than 400bp, easy to amplify, and suitable for the inspection of trace specimens; the alle...

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Problems solved by technology

For the 8-color and 9-color fluorescent channels, effective analysis cannot be carried out in my country, which has caused the overall situation of forensic DNA identification in China to lag behind

[0004] Disadvantages of the prior art: 1. At present, it is known in China that for the analysis of the original data of the short tandem repeat sequence STR 8 color and 9 color, due to the problem of the analysis method, there are phenomena such as missing peaks, wrong peaks, and misjudgments, and it is impossible to make accurate results. Gene sequencing analysis greatly hinders domestic DNA sequencing

2. It is not perfectly compatible with all common kits for insomnia, and needs to be adjusted separately for different kits to analyze the internal standard rules of fragment combinations

3. The judgment method set by the existing analysis software can only target the 5-color fluorescence channel. Due to the continuous improvement of reagent sensitivity, the number of detectable loci has also increased rapidly, and the 5-color fluorescence channel can no longer meet the requirements.

4. The currently used analysis method requires manual determination of certain peak types during the data analysis process, which requires the experimenter to have rich analysis experience to make accurate analysis and judgment, resulting in a high entry threshold for analysis, which cannot gain popularity

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