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Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis

An infrared spectrometer and attenuated total reflection technology, which is applied in the field of infrared spectroscopy, can solve the problems of low detection sensitivity, difficult production, and large sample volume required, and achieve the effect of simple raw materials, easy installation, and small sample volume

Inactive Publication Date: 2012-06-20
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention of this device is to solve the problems that the current infrared device is not suitable for DNA hybridization online analysis, difficult to manufacture, low detection sensitivity, and large sample volume. Enhanced Infrared Spectrometer Auxiliary Optical Device

Method used

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  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis
  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis
  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis

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Effect test

Embodiment 1

[0030] Preparation of infrared light window: take a silicon hemisphere 1 with a diameter of 35 mm, and coat the island-shaped nano-gold film 2 on the plane of the silicon hemisphere. For the preparation method of the island-shaped nano-gold film, please refer to: 1. Hiroto Miyake, Shen Ye, Masatoshi Osawa, Electroless deposition of gold thin films on silicon for surface-enhanced infrared spectroelectrochemistry, Electrochemistry Communications 4(2002) 973-977.

Embodiment 2

[0032] The infrared light window, the sealing ring 8, the fixing ring 6, the infrared spectrum pool 3 and the The fixed pressure tubes 7 are connected together, and the infrared spectrum pool 3 is a cylinder with a shoulder. Sockets, the inner diameter of the reference electrode socket is φ4mm, the internal diameter of the counter electrode socket is φ2mm, and the gold film is used as the working electrode, which can be applied to infrared spectroelectrochemical research; the other two are liquid sample inlets and outlets, the inner diameter is φ2mm, and the bottom is a miniature columnar storage There are two columns for the liquid pool, and the columns are cylinders with screw holes at both ends, with a diameter of φ10mm and a height of 21mm, and the bottom bracket 5 of the light window is a strip, such as figure 2 As shown, there is a disc with a hole in the center, which can support the silicon hemisphere. There are holes at both ends, and the distance between the center ...

Embodiment 3

[0034] The auxiliary optical device for DNA analysis attenuated total reflection surface-enhanced infrared spectrometer (ATR-SEIRA) optical path system schematic diagram is assembled in the ATR attachment. Rotate the fixed ring 6 to adjust the position of the light window in the ATR attachment. At the same time, by adjusting the angles of the two mirrors in the ATR attachment, the incident angle of the infrared beam can be changed so that the incident angle of the infrared beam is greater than the critical angle. At this time, the infrared beam The light beam is totally reflected on the inner surface of the light window. In this experiment, the incident angle is greater than 70°C. In the ATR-SEIRAS experiment, the p-polarized infrared light enters the light window at an incident angle greater than 70°C and undergoes total reflection on the inner surface of the light window. Fourier transform infrared spectrometer Tensor 27 (Bruke, Germany) was used for the acquisition of infr...

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Abstract

The invention relates to an attenuation total reflection surface enhanced infrared optical device for DNA identification and hybridization dynamics analysis. The attenuation total reflection surface enhanced infrared optical device comprises an attenuation total reflection surface enhanced infrared optical window in which an island-shaped nanometer gold film is plated on the surface of a silicon hemisphere, an optical table base, a fixed support region, an infrared light spectrum pool, a PDMS (Polydimethylsiloxane) sealing ring and the like, wherein the silicon hemisphere plated with the gold island-shaped film is placed on the optical table base, a gold film surface of the optical window is toward the infrared light spectrum pool, and the middle of the optical window is sealed by the PDMS sealing ring; the light spectrum pool, the PDMS sealing ring and the silicon hemisphere optical window are screwed-in and fixed through an external thread of the fixed support and an internal thread of an upper support of the optical table base so as to form the surface enhanced infrared spectrometer for DNA analysis. The testing device, provided by the invention, has the advantages of simple structure, convenience for operation and small required sample amount, and can be stably used for a long time and convenient for real-time on-line detection of infrared spectrum signals at a waveband of 1100cm<-1> to 4000cm<-1>, and high-sensitivity analysis of DNA molecules, so that DNA identification and hybridization mechanisms and kinetics research can be realized.

Description

Technical field: [0001] The invention belongs to the technical field of infrared spectroscopy, in particular to an attenuated total reflection surface enhanced infrared spectrometer auxiliary optical device for DNA analysis. Background technique: [0002] Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) is an important analytical tool for studying molecular structure information at interfaces. Combined with surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) in attenuated total reflection (ATR) mode, it has the advantages of strong surface signal, unimpeded mass transfer, and small interference from bulk solution. Research on recognition and hybridization kinetics. At the same time, it avoids the problems faced by traditional IR Absorption Spectroscopy (IRAS), such as insufficient surface signal, mass transfer supplementary lag, and interference from solution background. The use of internal reflection surface enhanced infrared absorption spectroscopy t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/35G01N21/3577
Inventor 夏兴华徐建云包文晶王康杨晨金波
Owner NANJING UNIV
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