The invention relates to an attenuation total reflection surface enhanced infrared optical device for DNA identification and hybridization dynamics analysis. The attenuation total reflection surface enhanced infrared optical device comprises an attenuation total reflection surface enhanced infrared optical window in which an island-shaped nanometer gold film is plated on the surface of a silicon hemisphere, an optical table base, a fixed support region, an infrared light spectrum pool, a PDMS (Polydimethylsiloxane) sealing ring and the like, wherein the silicon hemisphere plated with the gold island-shaped film is placed on the optical table base, a gold film surface of the optical window is toward the infrared light spectrum pool, and the middle of the optical window is sealed by the PDMS sealing ring; the light spectrum pool, the PDMS sealing ring and the silicon hemisphere optical window are screwed-in and fixed through an external thread of the fixed support and an internal thread of an upper support of the optical table base so as to form the surface enhanced infrared spectrometer for DNA analysis. The testing device, provided by the invention, has the advantages of simple structure, convenience for operation and small required sample amount, and can be stably used for a long time and convenient for real-time on-line detection of infrared spectrum signals at a waveband of 1100cm<-1> to 4000cm<-1>, and high-sensitivity analysis of DNA molecules, so that DNA identification and hybridization mechanisms and kinetics research can be realized.