Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis

An infrared spectrometer and a technique of attenuated total reflection, which is applied in the field of infrared spectroscopy, can solve the problems of high manufacturing difficulty, low detection sensitivity, and large sample amount required, and achieves the effect of simple and easy installation, simple raw materials and small sample amount.

Inactive Publication Date: 2014-04-30
NANJING UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the invention of this device is to solve the problems that the current infrared device is not suitable for DNA hybridization online analysis, difficult to manufacture, low detection sensitivity, and large sample volume. Enhanced Infrared Spectrometer Auxiliary Optical Device

Method used

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  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis
  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis
  • Auxiliary optical device of attenuation total reflection surface enhanced infrared spectrometer for DNA analysis

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Experimental program
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Effect test

Embodiment 1

[0030] Preparation of the infrared light window: Take a silicon hemisphere 1 with a diameter of 35mm, and plate the island-shaped gold nano-film 2 on the silicon hemispherical plane. For the preparation method of the island-shaped gold nano-film, see: 1. Hiroto Miyake, Shen Ye, Masatoshi Osawa, Electroless deposition of gold thin films on silicon for surface-enhanced infrared spectroelectrochemistry, Electrochemistry Communications 4(2002)973-977.

Embodiment 2

[0032] The infrared light window, the sealing ring 8, the fixed ring 6, the infrared spectrum pool 3 and the infrared spectroscopy pool 3 and the fixed ring 6, the sealing ring 8 and the fixed pressure tube 7 are connected by the column 4 on both sides of the silicon hemisphere and the light window bottom support 5 and the fixed pressure tube 7 at the bottom of the silicon hemisphere. The fixed pressure tubes 7 are connected together. The infrared spectrum pool 3 is a cylindrical body with a shoulder made of polytetrafluoroethylene. The diameter of the cylindrical body is φ28.7mm, the diameter of the shoulder is φ37mm, and the shoulder thickness is 4.5mm. There are four spectrometer cells. Sockets, the inner diameter of the reference electrode socket is φ4mm, the inner diameter of the counter electrode socket is φ2mm, and the gold film is used as the working electrode, which can be applied to infrared spectroelectrochemical research; the other two are the liquid sample inlet and ...

Embodiment 3

[0034] For DNA analysis, the auxiliary optical device of the attenuated total reflection surface enhanced infrared spectrometer is assembled in the ATR accessories according to the schematic diagram of the optical path system of the attenuated total reflection surface enhanced infrared spectrometer (ATR-SEIRA). Rotating the fixed ring 6 can adjust the position of the light window in the ATR accessory. At the same time, by adjusting the angles of the two mirrors in the ATR accessory, the incident angle of the infrared beam can be changed so that the incident angle of the infrared beam is greater than the critical angle. The light beam is totally reflected on the inner surface of the light window. In this experiment, the incident angle is greater than 70°C. In the ATR-SEIRAS experiment, p-polarized infrared light enters the light window at an incident angle greater than 70°C and is totally reflected on the inner surface of the light window. The Fourier Transform Infrared Spectro...

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Abstract

The invention relates to an attenuation total reflection surface enhanced infrared optical device for DNA identification and hybridization dynamics analysis. The attenuation total reflection surface enhanced infrared optical device comprises an attenuation total reflection surface enhanced infrared optical window in which an island-shaped nanometer gold film is plated on the surface of a silicon hemisphere, an optical table base, a fixed support region, an infrared light spectrum pool, a PDMS (Polydimethylsiloxane) sealing ring and the like, wherein the silicon hemisphere plated with the gold island-shaped film is placed on the optical table base, a gold film surface of the optical window is toward the infrared light spectrum pool, and the middle of the optical window is sealed by the PDMS sealing ring; the light spectrum pool, the PDMS sealing ring and the silicon hemisphere optical window are screwed-in and fixed through an external thread of the fixed support and an internal thread of an upper support of the optical table base so as to form the surface enhanced infrared spectrometer for DNA analysis. The testing device, provided by the invention, has the advantages of simple structure, convenience for operation and small required sample amount, and can be stably used for a long time and convenient for real-time on-line detection of infrared spectrum signals at a waveband of 1100cm<-1> to 4000cm<-1>, and high-sensitivity analysis of DNA molecules, so that DNA identification and hybridization mechanisms and kinetics research can be realized.

Description

Technical field: [0001] The invention belongs to the technical field of infrared spectroscopy, and is specifically an auxiliary optical device of an attenuated total reflection surface enhanced infrared spectrometer for DNA analysis. Background technique: [0002] Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) is an important analysis tool for studying molecular structure information of interfaces. Surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS) equipped with Attenuated Total Reflection (ATR) mode has the advantages of strong surface signal, unimpeded mass transfer and small interference from bulk solution, combined with surface selection law to facilitate the development of DNA molecules Research on recognition and hybridization dynamics. At the same time, it avoids the problems faced by traditional IR Absorption Spectroscopy (IRAS), such as insufficient surface signal, delayed mass transfer, and interference from solution background. The use of inte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01G01N21/3577
Inventor 夏兴华徐建云包文晶王康杨晨金波
Owner NANJING UNIV
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