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Rapid microorganism detecting device based on mie scattering

A technology of Mie scattering and detection device, which is applied in the measurement of scattering characteristics and other directions, can solve the problems of false positives, high cost, and long time in detection results, and achieve the effect of real-time rapid detection, realization of detection, and guarantee of detection accuracy.

Inactive Publication Date: 2015-12-09
NANJING INST OF ADVANCED LASER TECH
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Problems solved by technology

These methods are usually costly, requiring pre-cultivation of a certain number of microorganisms and adding specific culture reagents during the cultivation process, while the cultivation process takes a lot of time, and the possibility of false positive test results is high

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  • Rapid microorganism detecting device based on mie scattering

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Embodiment Construction

[0024] The present invention will be further described through the embodiments below in conjunction with the accompanying drawings, so as to better understand the present invention.

[0025] to combine figure 1 , the rapid detection device for microorganisms based on Mie scattering consists of a laser 1 , an optical focusing system 2 , a detector array frame 3 , a sample pool 4 and a photodetector 5 . The detector array frame 3 is a spherical cage structure composed of four identical semicircular arc-shaped frame arms 31. The diameter of the ball is 203mm, and the thickness of a single frame arm 31 is 26mm. The detection hole 32 used to install the detector; the laser wavelength of the laser 1 is 632nm, the power is adjustable from 0mW to 100mW, and the built-in collimation filter unit, the emitted laser is collimated parallel light; the focus of the optical focusing system 2 and the detector The center of the sphere of the array frame 3 coincides, the back focal length of th...

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Abstract

The invention discloses a rapid microorganism detecting device based on mie scattering. The rapid microorganism detecting device comprises a laser device, an optical focusing system, a detector array frame, a sample pool and photoelectric probes. Laser beams emitted by the laser device form parallel light after being aligned and are then focused on and irradiate microorganism samples in the sample pool through the optical focusing system, microorganisms emit scattered light to space after being irradiated by lasers, the scattered light is received by the photoelectric probes around the sample pool, received photoelectric signals are analyzed and calculated through a computer on the basis of the mie scattering theory, and the current form of the microorganisms can be detected in real time. By means of the rapid microorganism detecting device, the microorganisms are rapidly detected in real time; according to the optical scattering principle, the detection speed and accuracy are increased, signals are collected, processed and analyzed in real time in the collection process, and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a detection device for rapid detection of microorganisms by using Mie scattering of particles irradiated with light. Background technique [0002] Microorganisms are all tiny organisms that are invisible or unclear to the naked eye. They are small and simple in structure, and usually can only be seen clearly with an optical microscope and an electron microscope. They are collectively referred to as microorganisms. Microorganisms include bacteria, viruses, molds, yeasts, etc. The detection of microorganisms is of great significance in food safety, disease diagnosis and control, and biological science research. The rapid detection of microorganisms requires the detection of target microorganisms in the shortest possible time with high sensitivity and specificity and simple operation as possible. [0003] Traditional microbial detection methods mainly include cultured...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/51
Inventor 荣四海王炜刘宾李智洋何农跃
Owner NANJING INST OF ADVANCED LASER TECH
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