High-specific heat capacity microbe biological polysaccharide SM-1 and its preparation method and use

A technology with high specific heat capacity and biological polysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of slowing down the loss of body temperature and delaying the rise

Inactive Publication Date: 2015-04-08
杨红兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no suitable polysaccharide suitable for industrial production in terms of high specific heat capacity, especially anti-infrared radiation, so the present invention aims to fill up the gap in the art

Method used

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  • High-specific heat capacity microbe biological polysaccharide SM-1 and its preparation method and use
  • High-specific heat capacity microbe biological polysaccharide SM-1 and its preparation method and use
  • High-specific heat capacity microbe biological polysaccharide SM-1 and its preparation method and use

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Embodiment 1

[0024] The production microorganism selected in this embodiment is Bacillus subtilis. The source of Bacillus subtilis in this example is as follows: samples are collected from different plant surfaces (for example, yellow sunflower, aloe, etc.), and a medium with low nitrogen content (the medium is composed of components of the following quality: sucrose 10g, NaCl 0.2g, K 2 HPO 4 0.2g, GaCO 3 5g, MgSO 4 0.2g, (NH 4 ) 2 SO 4 0.1g, PH=7.0), the viscous and transparent strains are preserved for re-screening; re-screening is performed on the medium with lower nitrogen content above, and finally high-yielding polysaccharide strains are obtained. 16srDNA was identified as Bacillus subtilis, (see the DNA sequence list for 16srDNA sequence) as the starting strain. Further for the starting strain in the specific glycosyltransferase gene ( GumM versus GumH with GumK ) And GDP-mannose dehydrogenase gene algD After site-directed mutation and enhanced expression, a recombinant produ...

Embodiment 2

[0030] The monosaccharide composition of the high specific heat capacity microbial biopolysaccharide SM-1 obtained in Example 1 was determined.

[0031] The monosaccharide composition of the high specific heat capacity microbial biopolysaccharide SM-1 obtained in Example 1 was determined by gas chromatography. The results are shown in Figure 2(ab). Scan the reference sample: the monosaccharide components are in order of peak time: rhamnose, Galactose, xylose, mannose, glucose, arabinose, inositol (internal standard 1.75mg), the sample is mainly composed of 3 kinds of monosaccharides, compared with the peak time of the standard, it can be seen that these 3 peaks correspond to each other It is mannose, glucose and galactose. Calculating the area ratio of each peak in the sample by the method of integration shows that the molar ratio of mannose, glucose, and galactose is about 2:1.5:1.

Embodiment 3

[0033] The molecular weight of the high specific heat capacity microbial biopolysaccharide obtained in Example 1 was determined.

[0034] The molecular weight of BMPS was determined by high performance gel filtration chromatography (HPGFC), from image 3 The peak time in BMPS can be seen as a single peak, indicating that the purity of the polysaccharide solution is relatively high. After being determined by gel chromatography, the average molecular weight of BMPS is about 25,000 Da.

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Abstract

The invention belongs to the technical field of microbe polysaccharide and relates to high-specific heat capacity microbe biological polysaccharide and its preparation method and use. The preparation method comprises that a bacillus subtilis strain capable of effectively accumulating extracellular capsular polysaccharide is selected from nature and then is subjected to 16rs DNA identification, a polysaccharide synthesis approach-related gene is adjusted and modified so that a yield is improved, and through microbial fermentation, alcohol precipitation, and drying and refining, the high-specific heat capacity microbe biological polysaccharide is prepared. The high-specific heat capacity microbe biological polysaccharide has a structure skeleton mainly comprising glucose, galactose, mannose and glucuronic acid, has a side chain tail end obtained by connection polymerization of pyruvic acid and acetyl groups, is a polymer, has molecular weight of 5000-30000Da, is named as SM-1, has a good water retention capacity and a high-specific heat capacity, has moisture retention and infrared radiation resistance, and has a good application value in the cosmetic industry.

Description

technical field [0001] The invention belongs to the technical field of microbial polysaccharides, and in particular relates to high specific heat capacity microbial biological polysaccharides and a preparation method and application thereof. Background technique [0002] Specific heat capacity, also known as specific heat capacity, referred to as specific heat, is the heat capacity of a unit mass substance, that is, the heat energy absorbed or released by a unit mass object when the unit temperature is changed, usually expressed by the symbol c. Specific heat capacity is an important physical quantity, and the measurement of material specific heat capacity is one of the basic measurements in physics. Polysaccharides are a class of polymer compounds with complex structures and types formed by the condensation of many different types or different monosaccharide molecules. At the same time, they are considered to be one of the macromolecular substances involved in biological li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04A61K31/715A61P17/16A61K8/02A61K8/73A61K8/84A61K8/92A61Q17/04A61Q19/00A61Q19/10C12R1/125C12R1/01
Inventor 杨红兵
Owner 杨红兵
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