Plant tissue small sample rapid preparation method

Abstract

The invention relates to a plant tissue small sample rapid preparation method which is used for carrying out component analysis or DNA identification of plant fresh samples. According to the invention, a plant fresh sample is directly added into a 2ml round-bottom centrifuge tube, and is subjected to a bake-drying treatment in a baking oven or a fast-drying treatment in liquid nitrogen; the sample is directly crushed in the centrifuge tube by using a handheld drill with a dome bit; an extraction liquid is added into the bake-dried sample tube for leaching, or an extraction liquid is added into the frozen sample tube for decomposing cells; a supernatant is obtained by separation in a high-speed centrifuge; and the supernatant is adopted as a component analysis or DNA identification sample. With the method provided by the invention, sample preparation work is flexible, instrument operation is eliminated, steps are simplified, certain test cost is saved, and working efficiency is improved. The method provided by the invention is suitable to be used in tobacco potassium nutrition and potassium high-efficiency genetic material potassium content determination works, and in sample preparation works before molecular biology research DNA extraction. The method facilitates laboratory sample analysis and testing works with small samples and large sample analysis amounts.

Description

technical field [0001] The invention relates to a rapid sample preparation method for analyzing and measuring plant tissues, in particular to a method for directly crushing fresh or dried plant samples in a centrifuge tube. Background technique [0002] Potassium (K + ) is the mineral nutrient element with the highest content in plants, also known as "quality element", which can increase crop yield, improve crop quality, and enhance crop resistance. Tobacco is a potassium-loving plant, and potassium is also one of the important quality indicators of flue-cured tobacco. High potassium content in tobacco leaves has a good effect on the appearance quality, sensory quality and smoking safety of tobacco leaves. At present, the potassium content of tobacco leaves in the Huanghuai tobacco area and some southwest tobacco areas in my country is relatively low. It is one of the economical and effective technical ways to increase the potassium content of tobacco leaves in my country t...

AI Technical Summary

Problems solved by technology

Kits can be used to extract DNA from plant samples, but the extraction cost of each sample is relatively high, mainly for rapid sample preparation and DNA extraction of fresh plant samples with a small number of samples; another method is to use liquid nitrogen to freeze, and then magnetic beads High-frequency vibration is used to break the sample, and finally the DNA is extracted by the conventional CTAB or SDS method. There are many operating procedures, and the number of samples broken by the machine is small each time; the potassium content of plant tissue is mainly determined by flow analyzer and flame photometer. Determination method, but the sample preparation before the measurement on the machine usually uses a manual high-speed pulverizer to crush and dry the sample or use a mortar to grind the sample manually. The former requires a sufficient sampling amount for each sample, and the latter can grind a relatively small sample amount. samples, but the recovery rate is low, and there is a certain loss of sample volume. Both of them have large labor input and low work efficiency.

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