Species-specific primer pair, kit and identification method for quickly identifying Bactrocera scutellata

Abstract

The invention provides a species-specific primer pair, a kit and an identification method for quickly identifying Bactrocera scutellata. Sequences of specific primers JF190 and JR386 are JF190: 5'-CAGTTGGTTAGTACCCTTAGTACTCGG-3'; and JR386: 5'-GATCAACAGATGCTCCTCCATGGG-3', respectively. The invention has the advantages that the species-specific primer pair is more specific, is able to amplify only Bactrocera scutellata, is capable of amplifying a specific band 197bp in length, is unable to amplify closely related species of Bactrocera scutellata, and is higher in amplifying efficiency; operating is simple, fast and efficient; the species-specific primer pair is higher in sensitivity, has higher practical value, is capable of accurately, quickly and specifically detecting various states of Bactrocera scutellata and even wrack thereof, and has a wide detection range.

Description

technical field [0001] The invention belongs to the field of biochemistry and molecular biology, and in particular relates to a pair of species-specific primers, a kit and an identification method for quickly identifying Bacterophae spp. Background technique [0002] Bactroceras cutellata, Latin scientific name: Bactroceras cutellata (Hendel), belongs to Diptera Diptera tephritidae Tephritidae fruit fly genus Bactrocera, is a kind of important invasion and quarantine pests. At present, the distribution of Bactrocera spp. in the world is relatively limited, mainly in Vietnam (Gong Xiuze, 2003), India (Liang Richong et al., 1985), Myanmar (Deng Yuliang et al., 2005), Laos (Deng Yuliang et al., 2010), Japan (Liang Guangqin et al., 1989; Miyatake et al., 2000) and South Korea (Munetal., 2000) and other places. my country is one of the main distribution areas of B. tegus in the world. At present, it is mainly distributed in Taiwan, Guangdong, Guangxi, Fujian, Zhejiang, Jiangxi, ...

AI Technical Summary

Problems solved by technology

The main problems in this kind of identification method are: (1) it takes a long time to raise larvae, eggs or pupae into adults, which seriously affects the customs clearance speed of fruit and vegetable import and export trade; (2) if fruit f

Images