Method for identifying Frankliniella occidentalis species by utilizing PCR (Polymerase Chain Reaction)-RFLP (Restriction Fragment Length Polymorphism) technology

A technology of PCR-RFLP and western flower thrips, applied in the field of agricultural biology
CN102392075AInactive Publication Date: 2012-03-28INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
Publication Date
2012-03-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for identifying Frankliniella occidentalis species by utilizing PCR (Polymerase Chain Reaction)-RFLP (Restriction Fragment Length Polymorphism) technology, comprising the following steps: (1) extracting Frankliniella occidentalis genome DNA; (2) conducting PCR amplification to the mitochondrion COI gene of the Frankliniella occidentalis genome DNA by taking the Frankliniella occidentalis genome DNA as a template; (3) conducting enzyme cutting to the PCR product prepared in step (2) by adopting restriction enzyme EarI to obtain an enzyme cutting product; and (4) conducting agar gel electrophoresis analysis on the enzyme cutting production prepared in step (3). The restriction enzyme is the common restriction enzyme, therefore, the method provides a simple, convenient and stable enzyme cutting marker for screening two types of Frankliniella occidentalis, and simultaneously explores and establishes an identification technology for the two types of Frankliniella occidentalis, and lays a foundation for identification on the population dynamics of the two types of Frankliniella occidentalis and research on biology and invasion mechanisms.
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Description

technical field

[0001] The invention relates to a method for identifying thrips occidentalis strains by using PCR-RFLP technology, and belongs to the field of agricultural biotechnology. Background technique

[0002] Western flower thrips (Frankliniella occidentalis), which belongs to Thysanoptera (Thysanoptera) Thripidae (Thripidae) flower thrips (Frankliniella), originally originated in the western United States, and began to spread rapidly to all continents of the world in the 1980s. It has been reported in 69 countries and regions, and it is one of the most serious worldwide pests to crops (Kirk, 2001; Kirk and Terry, 2003). The pest not only feeds on the juice of the host plant, forms scars on the surface of the fruit, and reduces the quality of the fruit, but also can spread tomato spotted wilt virus (TSWV) and impatiens necrotic spot virus (INSV) in a persistent manner (Kritzman A, 2002 ). Moreover, the economic loss caused by the spreading virus is far greater than...

Claims

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