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Sinilabeo rendahli DNA (deoxyribonucleic acid) bar code standard detection gene and application thereof

A standard detection and barcoding technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of lack of data and shorten the identification time.

Active Publication Date: 2016-04-13
深圳华大海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the endemism of many fishes in our country, there is not enough relevant data

Method used

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  • Sinilabeo rendahli DNA (deoxyribonucleic acid) bar code standard detection gene and application thereof
  • Sinilabeo rendahli DNA (deoxyribonucleic acid) bar code standard detection gene and application thereof
  • Sinilabeo rendahli DNA (deoxyribonucleic acid) bar code standard detection gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The acquisition of embodiment 1 Chinese carp standard sequence

[0033] 1. Morphological identification and sample collection and preservation of rohu sinensis

[0034] According to the Chinese dace, it has body length, is slightly rod-shaped, and has a high and wide tail peduncle. Snout blunt and protruding, mouth inferior, transversely split. The front part of the upper lip is smooth, covered by the free kiss skin, and there are small papillae on both sides; the inner edge of the free part of the lower lip has many small papillae, the lower lip is separated from the lower jaw, and there is a deep groove in between, and the upper jaw is the upper lip packaged. There is 1 pair of short barbels, and the snout barbels are often degenerated. Dorsal fin iv-10; pectoral fin i-15-17; pelvic fin i-8; anal fin iii-5; dorsal fin front scales 18-24; pericaudal peduncle scales 22-24; lateral line scales 45-47. The back and sides of the body are blue-black, the scales are purple-...

Embodiment 2

[0054] 1. Collection, preservation and processing of samples to be tested

[0055] Part of the muscle tissue was cut from the soaked specimen, stored in 95% alcohol and stored in a -20°C refrigerator.

[0056] 2. DNA extraction

[0057] The DNA in the samples was extracted by the phenol-chloroform method and stored at -20°C for future use.

[0058] 3. PCR primer synthesis

[0059] This method selects the sequence of 2 pairs of primers as follows:

[0060] FishF2_t1:5'-TGTAAAACGACGGCCAGTCGACTAATCATAAAGATATCGGCAC-3' (SEQ ID NO.2)

[0061] VF2_t1:5'-TGTAAAACGACGGCCAGTCAACCAACCACAAAGACATTGGCAC-3' (SEQ ID NO.3)

[0062] FishR2_t1:5'-CAGGAAACAGCTATGACACTTCAGGGTGACCGAAGAATCAGAA-3' (SEQ ID NO.4)

[0063] FR1d_t1:5'-CAGGAAACAGCTATGACACCTCAGGGTGTCCGAARAAYCARAA-3' (SEQ ID NO.5)

[0064] 4. PCR reaction system (25ul)

[0065]

[0066]

[0067] 5. PCR reaction procedure

[0068] 95℃*2min,

[0069]

[0070] 72℃*10min

[0071] After the reaction, turn on the PCR instrument...

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Abstract

The invention discloses a sinilabeo rendahli DNA (deoxyribonucleic acid) bar code standard detection gene and application thereof. The DNA bar code standard detection gene is of a COI gene of which a nucleotide sequence is SEQ ID NO.1. A sinilabeo rendahli DNA bar code species identification method disclosed by the invention adopts a reliable DNA molecular identification technology, and the sinilabeo rendahli is rapidly and accurately indentified. Compared with a traditional morphological identification method, the standard detection gene sequence obtained by the method is conducive to realizing the molecular identification of the sinilabeo rendahli, and can effectively shorten the identification time.

Description

technical field [0001] The invention belongs to the field of animal molecular biology, and in particular relates to a DNA barcode standard detection gene of carp sinensis and an application thereof. Background technique [0002] Sinilabeorendahli, scientific name: (Sinilabeorendahli) is ray-finned fish class Cyprinidae, Cyprinidae, Sinillabeorendahli subfamily, Sinilabeorendahli. Commonly known as: Qinglong stick, peach blossom stick, wild dace, Qingyizi, is a unique species in China. The body is long, slightly rod-shaped, with a high and wide tail peduncle. Snout blunt and protruding, mouth inferior, transversely split. The front part of the upper lip is smooth, covered by the free kiss skin, and there are small papillae on both sides; the inner edge of the free part of the lower lip has many small papillae, the lower lip is separated from the lower jaw, and there is a deep groove in between, and the upper jaw is the upper lip packaged. There is 1 pair of short barbels,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/53
CPCC12Q1/6869C12Q1/6888C12Q2600/166C12Q2531/113C12Q2563/185
Inventor 朱志飞章晋勇石琼马兴宇王敏吴菲菲
Owner 深圳华大海洋科技有限公司
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