PCR special primer pair for identifying pig-cattle-sheep derived ingredients in livestock and poultry meat based on mitochondrion COI gene, PCR identification method and reagent kit

Abstract

The invention discloses a PCR special primer pair for identifying pig-cattle-sheep derived ingredients in livestock and poultry meat based on mitochondrion COI gene, an identification method and a reagent kit, and belongs to the technical field of food detection. According to difference sites of COI genes of 9 kinds of animals including pigs, cattle, sheep, rabbits, pigeons, quails, chickens, ducks and geese, specific primers of 3 kinds of animals including the pigs, the cattle and the sheep are designed and are shown in a sequence table SEQ ID NO. 1, a sequence table SEQ ID NO. 2, a sequence table SEQ ID NO. 3, a sequence table SEQ ID NO. 4, a sequence table SEQ ID NO. 5 and a sequence table SEQ ID NO. 6. The identification method comprises the following steps: extracting total DNA of species to be determined, and performing PCR amplification. The reagent kit comprises SEQ ID NO. (1-6). The primers of the PCR special primer pair disclosed by the invention perform specificity amplification only on objective fragments of respective species and cannot generate a reaction with other species; the method is high in sensitivity, simple, convenient and rapid, and the pig derived ingredients, the cattle derived ingredients and the sheep derived ingredients in the livestock and poultry meat can be effectively identified, so that the adulteration phenomena of beef and mutton are avoided. Besides, designed primers SEQ ID NO. (1-6) are matched with related reagents, so that the reagent kit can be made, and favorable industrialized production and application prospects can be obtained.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to the rapid and effective identification of porcine, cattle and sheep-derived components in livestock and poultry meat by using a PCR method, and is suitable for the rapid detection and authenticity identification of the above-mentioned three kinds of animal meat. Background technique [0002] With the rapid development of my country's food industry, there are more and more ways of food adulteration, more and more scope, more and more complex content. At present, in the sales of meat and meat products in the domestic market, there are frequent incidents of using adulteration and shoddy methods to deceive consumers to seek huge profits. For example, some unscrupulous enterprises use pork as raw material in order to seek improper benefits, and sell them as beef and mutton after processing. It was the exposure of the "beef jelly" incident that brought meat adulteration further...

AI Technical Summary

Problems solved by technology

However, there are no relevant reports on the establishment of a method for identifying pig, cattle, and sheep-derived components in livestock and poultry meat based on the mitochondrial DNACOⅠ gene

Images