PCR method for rapid identification of four types of blue crabs of scylla

A mud crab, rapid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of poor labeling repeatability, difficult to master, low identification accuracy, etc., to improve efficiency and accuracy, Simple to use effects
CN101792809AInactive Publication Date: 2010-08-04EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2010-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a PCR method for the rapid identification of four types of blue crabs of Scylla, comprising the following steps: extracting the genome DNA of the four types of the blue crabs; (2) designing a species specific amplification primer according to the nucleotide sequence of the chondriosome (COI) gene of the four types of the blue crabs; (3) carrying out PCR amplification on the genome DNA of the four types of the blue crabs; and (4) utilizing 1.5% of agarose gel electrophoresis to detect PCR products, so as to rapidly identify the four types of the blue crabs according to the different sizes of the products. The method has simple and safe operation, clear amplification stripe and true and reliable results, and can rapidly identify the four types of the blue crabs, thus being widely used in the fileds of protection and management of blue crab germ plasm resources, inheritance breeding and the like.
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Description

technical field

[0001] The invention belongs to the field of species identification of the genus Scylla, a marine economic crab, and particularly relates to a PCR method for rapidly identifying four species of Scylla genus. Background technique

[0002] The genus Scylla belongs to the phylum Arthropoda, the class Crustacea, the order Decapoda, and the family Portunidae, including 4 species: Scylla paramamosain and Scylla serrata. (Scyllaserrata), Scylla olivacea and Scylla tranquilbarica are mainly distributed in the tropical, subtropical and temperate regions of the Pacific Ocean and the Indian Ocean. In my country, it is mainly distributed in the southeast coastal provinces, with the most in Fujian, Guangdong and Hainan. It is one of the important cultured crabs in my country. In order to identify and protect the resources of blue crabs widely distributed in my country, Ma Lingbo et al. (2006) used the mitochondrial COI gene sequence to study the phylogenetic relationship...

Claims

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