Specific primers for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene

A technology of Anoptera and mitochondria, which is applied in the direction of genetic engineering, plant gene improvement, application, etc., to achieve the effect of reducing experiment cost, improving accuracy, and shortening experiment cycle

Inactive Publication Date: 2015-09-30
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researcher to compare DNA sequence patterns between various orders within one family or other type of animal called pteryxium (the stalk). By doing it with these techniques, scientists could make more accurate comparisons about how similar animals were made from each group compared against previous methods like histological observation at cellular level.

Problems solved by technology

Technological Problem: Current Methodologies For Identification Of Chlamydrates (Cladopters) However, these current methods have limitations that make them difficult to apply universally over different areas where CHLB occurs naturally occurring throughout the globe.

Method used

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  • Specific primers for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene
  • Specific primers for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene
  • Specific primers for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene

Examples

Experimental program
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Embodiment Construction

[0013] The amplification primer screening method of the Coeloptera COI gene of the present invention is divided into two steps: 1. Based on the sequence of the COI gene in the mitochondrial genome sequence of the Cooptera insect that has been measured, find out the COI gene by comparison The relatively conserved sequence fragments at both ends do not rely on the existing universal primers for insects, and design special primers for the order of the order; 2. Amplify the COI gene of the insects of the order of the order with the synthesized primers, and test its generality in the order of the order. 3. Many pairs of primers have been designed in the process, and the verification of experimental results shows that the primers of the present invention are optimal

[0014] Below in conjunction with example the invention is further described:

[0015] 1. Sample preparation: The samples were all insects of different families of the order Coptera stored in the specimen room of the In...

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Abstract

The invention relates to primers used for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene sequences, the plecoptera insect mitochondrion COI gene sequences are as shown in SEQ ID No.1 and SEQ ID No.2. The primers for amplifying are adopted, so as to amplify COI genes of various plecoptera species effectively, be conducive to accurately identifying close species difficult to indentify in some forms, and an important tool for classification and identification, phylogeny, population genetic structures and geographical population identification of plecoptera insects in China is provided.

Description

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Claims

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Application Information

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Owner YANGZHOU UNIV
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