PCR amplification kit for detecting clonorchis sinensis metacercaria on basis of plastosome COI genes and amplification primer

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) amplification kit for detecting clonorchis sinensis metacercaria on the basis of plastosome COI genes and an amplification primer. The PCR amplification kit comprises PCR amplification reaction liquid, wherein the PCR amplification reaction liquid includes 10mmol / L of Tris-HCl, 50mmol / L of KCL, 1.5mmol / L of MgCl<2>, 0.8mmol / L of dNTPs, 0.4[mu]mol / L of positive primers, 0.4[mu]mol / L of reverse primers, 20ng / [mu]L of DNA (Deoxyribonucleic Acid) templates and 0.075U / [mu]L of Taq enzyme. The PCR amplification kit has the advantages of high sensitivity, high specificity, high detection speed and high detection accuracy, and an effective technical measure is provided for diagnosis, treatment and prevention of clonorchis sinensis infection.

Description

technical field [0001] The invention relates to the technical field of parasite detection, in particular to a PCR amplification kit and amplification primers for detecting Clonorchis sinensis metacercariae based on the mitochondrial COI gene. Background technique [0002] Clonorchiasis (Clonorchiasis) is a food-borne zoonotic parasitic disease caused by infection with Clonorchis sinensis (Cs), which seriously endangers human health. The disease is mainly distributed in some countries in East Asia, Southeast Asia and Eastern Europe, such as China, South Korea, Laos and Vietnam. According to statistics, about 35 million people in the world are infected with this trematode, and 15 million people are distributed in various provinces and municipalities of my country (except Inner Mongolia, Qinghai, Tibet and Xinjiang), especially in Guangdong, Guangxi and the three northeast provinces. At present, there is no unified standard for the identification method of Clonorchis sinensis,...

AI Technical Summary

Problems solved by technology

As we all know, microscopic examination is time-consuming, low-efficiency, and requires high clinical work experience for the examiner. Moreover, for the metacercariae that are closely related to Clonorchis sinensis, the eggs are similar in shape and cannot be distinguished by ordinary light microscopy. misjudgment

Immunodiagnostics, although more efficient than microscopy, are often limited by sensitivity and specificity

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