Verification method of sea horses

Abstract

The invention relates to a verification method of sea horses, belonging to the field of molecular biology. The invention aims to provide a verification method of sea horses. The method is characterized by comprising the following steps: a. extracting sea horse total DNA (deoxyribonucleic acid); b. PCR (deoxyribonucleic acid) amplification: carrying out PCR amplification on the sea horse total DNA to obtain a COI gene sequence; c. carrying out PCR product sequencing; and d. carrying out sequence comparison to determine the species of the sea horse. The method is free from the restriction of morphological characteristics, can identify the species of the sea horse under the condition of being incapable of utilizing the traditional morphological classification process due to lack of morphological characteristics, has the advantages of high accuracy and the like, provides scientific references for sea horse systematics and sea horse resource researches in China, and also provides reliable references for biological diversity of sea horse resources and conservation of resources.

Description

technical field [0001] The invention relates to a method for identifying hippocampus and belongs to the field of molecular biology. Background technique [0002] At present, there are mainly the following common identification methods for Chinese herbal medicines: genetic source identification, trait identification, microscopic identification and physical and chemical identification, etc., but these identification methods have certain defects: (1) Phenotypic plasticity and genetic variability of Chinese medicinal materials It is easy to cause errors in identification results; (2) limited by biological sex and developmental stage; (3) it is impossible to accurately identify hidden taxa existing in many groups; (4) modern interactive identification systems require high professional technical knowledge and According to experience, most chemical methods cannot be used for species identification. At the same time, there are almost zero systematic and morphological studies on the...

AI Technical Summary

Problems solved by technology

[0002] At present, there are mainly the following common identification methods for Chinese herbal medicines: genetic source identification, trait identification, microscopic identification and physical and chemical identification, etc., but these identification methods have certain defects: (1) Phenotypic plasticity and genetic variability of Chinese medicinal materials It is easy to cause errors in identification results; (2) limited by biological sex and developmental stage; (3) it is impossible to accurately identify hidden taxa existing in many groups; (4) modern interactive identification systems require high professional technical knowledge and Experience, most chemical methods cannot be used for species identification

"Chinese Fish System Retrieval" classified the 6 species of Hippocampus in my country according to relatively simple morphological characteristics, which cannot be called an important basis for the identification of hippocampus morphology in my country; Except for the detailed description of H. kelloggi, the other four species are relatively brief and cannot be effectively and accurately identified; "Chinese Fauna" · Hydrasauridae has not yet been published, and it is impossible to describe the species of the genus Hippocampus in my country. Accurate species identification has a guiding role

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