Verification method of sea horses

An identification method and hippocampal technology, applied in the field of molecular biology, can solve problems such as inability to accurately identify species in animals, high professional technical knowledge and experience, biological sex and developmental stage restrictions, etc., so as to improve clinical drug safety and improve circulation. Status Quo, Effect of Strengthening Control

Inactive Publication Date: 2014-09-03
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF1 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there are mainly the following common identification methods for Chinese herbal medicines: genetic source identification, trait identification, microscopic identification and physical and chemical identification, etc., but these identification methods have certain defects: (1) Phenotypic plasticity and genetic variability of Chinese medicinal materials It is easy to cause errors in identification results; (2) limited by biological sex and developmental stage; (3) it is impossible to accurately identify hidden taxa existing in many groups; (4) modern interactive identification systems require high professional technical knowledge and Experience, most chemical methods cannot be used for species identification
"Chinese Fish System Retrieval" classified the 6 species of Hippocampus in my country according to relatively simple morphological characteristics, which cannot be called an important basis for the identification of hippocampus morphology in my country; Except for the detailed description of H. kelloggi, the other four species are relatively brief and cannot be effectively and accurately identified; "Chinese Fauna" · Hydrasauridae has not yet been published, and it is impossible to describe the species of the genus Hippocampus in my country. Accurate species identification has a guiding role

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Verification method of sea horses
  • Verification method of sea horses
  • Verification method of sea horses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Further, the sequence amplified by PCR was compared with the hippocampus sequence in the gene bank to identify the species of hippocampus.

[0064] In this embodiment, the hippocampus medicinal material is a dry product, which is purchased from the professional market of Hehuachi Chinese medicinal materials in Chengdu, the professional market of Chinese medicinal materials in Anguo, Hebei, and the professional market of Chinese medicinal materials in Bozhou, Anhui (hereinafter abbreviated as Hehuachi, Anguo, and Bozhou). The specific conditions are as shown in the table 1. Sampling is based on the phenotypic characteristics of the hippocampus, and the classification of the hippocampus mainly refers to the hippocampal classification and identification method of Lourie et al. (Lourie S A, Vincent A C J, Hall H J. Seahorses: an identification guide to the world's species and their conservation[M]. Project Seahorse, 1999; Foster S J, Cooper E W T, Vincent A C J. A guide to t...

Embodiment 2

[0164] 1. Experimental materials and reagents

[0165] Medicinal materials: hippocampus medicinal materials were purchased from the Chengdu Hehuachi Traditional Chinese Medicine Market. The hippocampus medicinal materials with inconspicuous identification features or damaged bodies that could not be identified accurately were selected as samples for experiments. The samples were numbered: Chengdu Hehuachi S01, Chengdu Hehuachi S02 , Hebei Anguo S03, the specific situation is as follows Figure 11 .

[0166] 2. Experimental method

[0167] 2.1 Total DNA extraction

[0168]2.1.1 DNA extraction

[0169] The total DNA of the hippocampus was extracted using the kit method in Example 1.

[0170] 2.1.2 Electrophoretic detection

[0171] The agarose gel with a concentration of 1.0% was used for electrophoresis under the conditions of constant voltage 150v and current 400mA, and the electrophoresis time was 10-20min. The gel plate was photographed in an automatic gel imager, and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a verification method of sea horses, belonging to the field of molecular biology. The invention aims to provide a verification method of sea horses. The method is characterized by comprising the following steps: a. extracting sea horse total DNA (deoxyribonucleic acid); b. PCR (deoxyribonucleic acid) amplification: carrying out PCR amplification on the sea horse total DNA to obtain a COI gene sequence; c. carrying out PCR product sequencing; and d. carrying out sequence comparison to determine the species of the sea horse. The method is free from the restriction of morphological characteristics, can identify the species of the sea horse under the condition of being incapable of utilizing the traditional morphological classification process due to lack of morphological characteristics, has the advantages of high accuracy and the like, provides scientific references for sea horse systematics and sea horse resource researches in China, and also provides reliable references for biological diversity of sea horse resources and conservation of resources.

Description

technical field [0001] The invention relates to a method for identifying hippocampus and belongs to the field of molecular biology. Background technique [0002] At present, there are mainly the following common identification methods for Chinese herbal medicines: genetic source identification, trait identification, microscopic identification and physical and chemical identification, etc., but these identification methods have certain defects: (1) Phenotypic plasticity and genetic variability of Chinese medicinal materials It is easy to cause errors in identification results; (2) limited by biological sex and developmental stage; (3) it is impossible to accurately identify hidden taxa existing in many groups; (4) modern interactive identification systems require high professional technical knowledge and According to experience, most chemical methods cannot be used for species identification. At the same time, there are almost zero systematic and morphological studies on the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2531/113C12Q2563/185C12Q2565/125
Inventor 国锦琳温珑莲侯飞侠万德光彭成
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap