277results about How to "Good fluorescence properties" patented technology

A multifunctional nano-class magnetic fluorescent material is prepared through wrapping nano fluorescent material (CdSe/ZnS, 4-8 nm) and nano magentic material (gamma-Fe2O3, 4-20nm) by high-molecular microbead in water or oil phase. Its advantage is high intensity and long life of fluorescence.

The invention relates to a preparation and application of a new macromolecule lipidosome. The structure is a vesicle which has a lipide dual-layer structure containing duoparental chitosan longchain alkyl quaternary ammonium salt as well as phospholipid with micromolecule. The chitosan longchain alkyl quaternary ammonium salt is characterized in that: the weight average molar mass is bigger than 2000; the chitosan longchain alkyl quaternary ammonium salt can be dissolved in chloroform and other organic solvent, which contains carboxyl, amino or quaternary ammonium salt radical and concretely is one of the duoparental chitosan longchain alkyl quaternary ammonium salt disclosed by 200710056993.4 of China Invention Patent. The surface of the macromolecule lipidosome can be connected with various Liver-Targeted preparations. The macromolecule lipidosome can carry water-soluble, oil-dissolving or duoparental materials including medicine, protein, gene, nutrition, vitamin, magnetic granule and quantum dot, etc. The macromolecule lipidosome has the advantages of high wrapping rate, simple and convenient operation, strong applicability and low cost; the macromolecule lipidosome after being covered has even distributed granule diameter which can be lower than 20nm for the smallest, has stable system and strong functions for carrying medical tiny balls and slowly controlling as well as releasing.

This invention provides a light-emitting diode which is capable of extracting white light, manufactured readily and highly reliable. The light-emitting diode is fabricated by laminating a buffer layer 2, an Si (silicon)-doped GaN fluorescent layer 3, an n-GaN layer 4, an MQW emission layer 5 and a p-GaN layer 6 on a sapphire substrate 1 in this order. The Si-doped GaN fluorescent layer 3 is doped higher concentration of Si (silicon) than a conventional Si-doped GaN layer with sufficient good crystallinity. Such a Si-doped GaN fluorescent layer is excited by blue light generated in the MQW emission layer and emits yellow light. The yellow light is the complement for the blue light generated in the MQW emission layer. White light can be obtained by blending and extracting both of blue light and yellow light.

The invention relates to a preparation method of magnetic fluorescence dual-function silicon oxide hollow microspheres, comprising the steps of: preparing magnetic nanometer particles by a coprecipitation method; diffusing the magnetic nanometer particles in long-chain alkane after the surfaces of the magnetic nanometer particles are modified by oleic acid; mixing the oil phase composed of alkanedispersion consisting of a styrene monomer, a superhydrophobic agent and magnetic nanometer particles, and the orthosilicic acid alkyl ester with water phase in which a surface active agent is dissolved; pre-emulsifying and finely emulsifying the mixture to obtain a fine emulsion drop system; when the drops are in free radical polymerization, adding an alkali catalyst to control the generation ofsilicon oxide and the phase separation of the organic and inorganic components of the system; in the reaction process, adding proper ammonia water and a silane coupling agent which is marked by fluorescein to obtain the hollow compound microspheres which are different in sizes, inorganic shell thicknesses and magnetic particle solid content and have stable fluorescence signals. The preparation method disclosed by the invention is simple, the raw materials are low in cost and easy to obtain; and the obtained fluorescence dual-functional hollow silicon oxide microspheres are narrow in size distribution, high in magnetic substance content and stable in fluorescence performance.

The invention relates to a synthesizing method of a functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application. The synthesizing method is characterized in that HAuCl4 is used as a precursor of Au (copper) atom, the BSA is used as a reductant and a protectant, and BSA-AuNCs is synthesized by a one-pot synthesis method. The synthesizing method has the beneficial effects that the synthesizing method is simple, and the reaction conditions are wild; the synthesized fluorescent AuNc has the advantages of good biocompatibility and photostability, good fluorescent characteristics, high yield of quantum, good water solubility, non toxicity, little inference by outside and the like; the synthesized BSA-AuNCs has higher selectivity on Cu<2+>, and can be applied to the quantitative detection and analysis of Cu<2+>; the detection method is simple and quick, the sensitivity is high, the toxicity is avoided, and the real-time qualitative and quantative detection of Cu<2+> is realized.

The invention provides a fluorescence immunochromatography test strip prepared by taking an aggregation-induced emission fluorescent microsphere as a beacon carrier. Filter paper, a sample cushion, anAIEFM (Aggregation-Induced Emission Fluorescent Microsphere) compound glass fiber cushion, a nitrocellulose membrane sprayed with a detection line and a quality control line, and water adsorption paper are overlapped and pasted to a bottom plate in sequence to prepare the fluorescence immunochromatography test strip, the fluorescence immunochromatography test strip is used for quantitatively detecting the concentration of an object to be tested in a sample, and the prepared test strip has the characteristics of being high in detection sensitivity.

A new class of nanostructured RE-doped SiO₂-base materials that display superior fluorescence properties is provided. In particular, high gain combined with a broad and flat spectral band width is observed in material composed of a high fraction of a nano-dispersed metastable silicate phase in a glassy SiO₂ matrix, produced by partial devitrification (crystallization) of several glassy Al₂O₃/Er₂O₃- and Y₂O₃/Er₂O₃-doped SiO₂ compositions. Also, a highly deconvoluted spectral emission, with several prominent peaks, is observed in completely devitrified material, consisting of a uniform nano-dispersion of an equilibrium silicate phase in a crystobalite SiO₂ matrix. Such enhanced fluorescence properties were observed in heat treated nanopowders prepared by vapor-phase, solgel, rapid solidification, and spray-pyrolysis methods.

The invention, which belongs to the field of biochemistry, relates to a tumor-targeting functionalized quantum dot and a preparation method thereof. The functionalized quantum dot consists of folic acid, cyclodextrin and quantum dot, and part of folic acid molecules are contained in the hydrophobic cavity of the cyclodextrin to form folic acid-cyclodextrin inclusion compound, which is then combined with the surface of the quantum dot. In the process of preparing the functionalized quantum dot, the folic acid is first combined with the Gamma-cyclodextrin to form the inclusion compound, and the Gamma-cyclodextrin-folic acid inclusion compound is then modified on the surface of the quantum dot, so that the dual-function nano material with excellent fluorescent characteristic and tumor-targeting property is produced. Since the Gamma-cyclodextrin is used to include the folic acid, the water-solubility and dispersibility of the folic acid are improved. The folic acid and the quantum dot are connected together by an effective method, and the produced fluorescent probe can be used for biological research on tumor cells. The preparation method is simple, the cost is low, and the screening of a variety of tumor cells and an in-vivo experiment on mice indicate that the material is characterized by low toxicity, high selectivity and the like.

A retroreflective graphic article bearing a fluorescent legend comprising a retroreflective base sheet, an image layer containing one or more pigments and one or more fluorescent dyes and a dye-receiving layer containing fluorescent dye which migrated from the image layer. Also, a method for making such articles is provided.

The invention discloses a specific fluorescent probe for identifying hydrazine and an application thereof, belonging to the field of fine chemical engineering. The fluorescent probe is a 4-trifluoromethyl-7-aminocoumarin derivative, and is prepared by steps of putting 4-trifluoromethyl-7-aminocoumarin, phthalic anhydride and sodium acetate into a reaction bottle in proportion, and performing backflow by adding acetic acid. The fluorescent probe and the corresponding hydrazine content detection process cannot be interfered by biological system matrixes and impurities, so the fluorescent probe can be used for quantitative measurement of the hydrazine content in various biological systems. The probe has high specificity, and can be subjected to hydrolysis after specifically reacting with hydrazine; a hydrolysate has good fluorescence characteristics. Raw materials are cheap and easily available, can be obtained through chemical synthesis, and synthesis process is simple and feasible; the probe has high sensitivity, is suitable for detecting the hydrazine content in cells, and can be used for measuring the hydrazine content by drawing a standard curve. The probe is a ratio type probe, and effectively avoids the influences of non-uniform probe distribution, environmental factors, actuators and the like on the measurement results.

The invention discloses a method for producing fluorescent/colorimetric dual-pattern paper chips for detection of cancer cell and cell surface polysaccharide. According to the method, the paper chips are produced by a wax printing technology, gold nanorods and porous silver and aptamers are grown in a fluorescent layer work area, then cancer cell capture is carried out, a multi-branched hybridization technology is used for amplification of signal molecule, and accurate measurement of the cancer cells can be realized, the produced paper chips are folded, a chromogenic solution is dropped in a reaction area, and thereby visualization pre-measurement of the cancer cells can be realized. A polysaccharide inhibitor is added, and dynamic symptom of cell surface polysaccharide is realized.

A method for visualizing hidden fingerprint includes preparing CdTe fluorescent nanoparticles, using glass atomizer to spray nanoparticles solution onto fingerprint carrier surface carried with fingerprint, using cyanoacylate to stream surface of fingerprint carrier to form reinforced film of fingerprint and using ultraviolet ray fluorescent photograph means to obtain fluorescent fingerprint image. The visualizing system used for realizing said method is also disclosed.

A compound represented by the following general formula (I) or a salt thereof:

wherein R¹, R², R³, and R⁴ independently represent 2-quinolyl group which may be substituted, 1-isoquinolyl group which may be substituted, or 3-isoquinolyl group which may be substituted; m, n, p, and q independently represent 1 or 2; and L represents a single bond, or an alkylene group having 1 to 5 carbon atoms wherein said alkylene group may contain one or more heteroatoms in the main chain, and may have one or more substituents on the main chain, which is useful as a fluorescent zinc ion sensor.

The invention discloses temperature-responsive AIE fluorescent polymer nanoparticles, a synthesis method and an application thereof. 1) synthesis of TPE-OH; 2) synthesis of TPE-BPM; 3) synthesis of TPE-PNIPAM: dissolving N-isopropylacrylamide in a mixed solvent with stirring, and successively adding tris(2-dimethylaminoethyl)amine, CuBr and TPE-BMP under argon protection, stirring the mixture at room temperature, dialyzing the reaction stock solution with ultrapure water and freeze-drying the dialyzate to obtain a slight-yellow solid powder, the TPE-PNIPAM. Through atom transfer radical polymerization (ATRP) for introducing synthesis of the fluorescent polymer having temperature sensitivity, the polymer, in a water solution, can be self-assembled into nano-particles and shows excellent water solubility and fluorescence characteristic.

A process for preparing fluorescent metal nano-particles belongs to the process for preparing nano-materials with fluorescent property, which aims to avoid needless and harsh preparing conditions to gain the nanometer metal particles which are modified by dimercapto compounds, emit royal purple fluorescence and provided with high quantum efficiency. The process for preparation is simple in operation, easy in fetching raw materials and low in price. The sequence of the invention includes the step of preparing reversed-phase micromulsion, titrating step and separating step. Gained final products are dispersed in organic solvent of normal hexane, chloroform and octane, and the final products are capable of emitting royal purple fluorescence under the shining of an ultraviolet lamp. The invention has the advantages of simple and safe operation, good repeatability, safe and available raw materials and low price, further, the achieved products are relatively good in dispersiveness, the fluorescent property is greatly modified, the quantum efficiency increases from 10-4 to about 12%, and the stability of the product is high. The invention has a potential application prospect in the respects of optical storage, full-color display and the like.

The invention belongs to the technical field of a biosensor and specifically relates to a fluorescence sensor as well as a preparation method and application thereof. A carbon quantum dot is utilized as a fluorescent material, a monoclonal antibody and an aptamer are simultaneously combined with a target object and the fluorescence sensor with a sandwich type structure is lastly acquired; the aptamer of the target object is arranged on the upper part of the fluorescence sensor; the target object is arranged on the middle part; the monoclonal antibody of the target object is arranged on the lower part; a sulfydryl group is modified on the aptamer of the target object; the carbon quantum dot is combined with the aptamer of the target object under the effect of hydrogen bonds. The invention also discloses a preparation method of the fluorescence sensor. The fluorescence sensor prepared according to the invention can be used for quantitatively determining the concentration of toxic fungus and toxin, especially quantitative determining the concentration of aspergillus flavus B1, has high stability and specificity, is wide in detection scope and is more accurate.

The invention discloses a multifunctional carrier based on cytogenic vesicle in body fluid, a preparation method and an application. The method comprises the following steps: (1) collecting human body fluid, performing gradient ultra-centrifugation, removing supernatant and using sterile PBS heavy suspension for precipitating and (2) adding the cytogenic membrane vesicle from the body fluid, ultra-small luminescent material and electroporation buffer solution into an electric shock cup, uniformly mixing, placing onto an electroporation instrument and treating, placing under room temperature and repairing after the ending of electroporation, performing ultra-centrifugation and then using sterile PBS heavy suspension for precipitating, thereby acquiring the multifunctional carrier based on the cytogenic membrane vesicle from the body fluid and marked with the ultra-small luminescent material. The carrier is used for preparing a chemotherapeutic drug or gene therapeutic drug for treating or preventing tumor, has high biocompatibility, is convenient to carry various therapeutic drugs, is capable of effectively restraining tumor growth, is suitable for diagnosis and treatment of various innocent and malignant tumors and especially supplies a choice for the diagnosis and treatment of deep tumors in skull, thoracic cavity and abdominal cavity.

The invention relates to an amphiphilic polymer fluorescent material (PAF-G) and a synthesis method thereof, belonging to the technical field of chemical synthesis. The synthesis method of the PAF-G comprises the steps of firstly, synthesizing perfluorophenyl polyacrylate (PFPA) by using a reversible addition-fracture chain transfer method; then, carrying out an aminolysis reaction by using the PFPA, and grafting tetraphenyl ethylene and polyethylene glycol groups onto a polymer side chain according to a proportion so as to obtain the PAF-G. The assembly performance, water solubility and luminescence property of the PAF-G are regulated and controlled by regulating and controlling the length of a main polymer chain, the polymerization degree of the polyethylene glycol and the proportion ofthe two grafted groups. The molecular weight of the PFPA, the ratio of tetraphenyl ethylene to polyethylene glycol and the length of the polyethylene glycol have a wide regulation and control space; compared with small molecules, the PAF-G shows higher fluorescence intensity and fluorescence quantum yield; PAF-G assembly has good biocompatibility and can be applied to cell imaging.

The invention relates to a fluorescence sensor for rapid detection of explosives and a preparation method thereof, and a method for rapid detection of explosives, wherein the fluorescence sensor and the method especially have high selectivity and sensitivity in trace detection of picric acid. The fluorescence sensor is a solution having a concentration of 1*10<-4> to 1*10<-6> mol/L and prepared by dissolving a graphene composite material in a solvent. The graphene composite material comprises graphene and a polymer as shown in a formula I which is described in the specification, wherein the polymer modifies graphene in a non-covalent bond manner via in-situ reduction of graphene oxide. When the concentration of picric acid is in a range of 1.3*10<-3> to 3.3 ppm, reduction in the fluorescence intensity of the fluorescence sensor is in linear relation with the concentration of a nitro-containing explosive, and quantitative determination can be realized. The fluorescence sensor has good fluorescence characteristics and AIE effect, can realize qualitative and quantitative determination of trace picric acid and is high in sensitivity and low in cost. The method for rapid detection of explosives is simple to operate and has good application prospects.