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Synthesizing method of functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application

A technology of bovine serum albumin and nano-gold clusters, which is applied in the field of functionalized nano-gold clusters as fluorescent probes to detect Cu2+, can solve the problems of cumbersome analysis steps, long analysis and testing cycles, complicated raw material pretreatment, etc., and achieves water solubility. Good, good biocompatibility and photostability, simple and fast detection method

Inactive Publication Date: 2017-11-24
XIAN UNIV OF SCI & TECH
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Problems solved by technology

However, excessive intake of copper in the human body can also cause a series of poisoning phenomena such as nausea, vomiting and acute hemolysis.
[0003] At present, although there are many detection methods for copper ions, these methods generally require large-scale and expensive analytical instruments, and the operation is inconvenient. Moreover, these methods also have long analysis and test cycles, complicated raw material pretreatment, and analysis steps. It is cumbersome and cannot perform real-time on-site detection of water areas and other shortcomings

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  • Synthesizing method of functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application
  • Synthesizing method of functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application
  • Synthesizing method of functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application

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Embodiment Construction

[0039] The technical solution of the present invention will now be further described in conjunction with the drawings and embodiments.

[0040]1. Synthesis of functionalized gold nanoclusters modified with bovine serum albumin

[0041] Take 2mL of bovine serum albumin solution with a concentration of 50mg / mL and 2mL of chloroauric acid solution with a concentration of 10mmol / L, add them to a 50mL round-bottomed flask in turn, stir and mix evenly with a collector type constant temperature heating magnetic stirrer, after 2min, add 0.2mL of 1mol / L NaOH solution, put the mixed solution in a water bath at 60°C for 6h, respectively, and continuously magnetically stir for 6h. After the reaction, brown functionalized gold nano-clusters can be obtained, which can be stored in a refrigerator (4°C) away from light spare.

[0042] The morphology and properties of the synthesized functionalized gold nanoclusters (BSA-AuNC for short) were characterized by the following experiments.

[004...

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Abstract

The invention relates to a synthesizing method of a functionalized AuNc (gold nanocluster) based on BSA (bovine serum albumin) and application. The synthesizing method is characterized in that HAuCl4 is used as a precursor of Au (copper) atom, the BSA is used as a reductant and a protectant, and BSA-AuNCs is synthesized by a one-pot synthesis method. The synthesizing method has the beneficial effects that the synthesizing method is simple, and the reaction conditions are wild; the synthesized fluorescent AuNc has the advantages of good biocompatibility and photostability, good fluorescent characteristics, high yield of quantum, good water solubility, non toxicity, little inference by outside and the like; the synthesized BSA-AuNCs has higher selectivity on Cu<2+>, and can be applied to the quantitative detection and analysis of Cu<2+>; the detection method is simple and quick, the sensitivity is high, the toxicity is avoided, and the real-time qualitative and quantative detection of Cu<2+> is realized.

Description

technical field [0001] The invention belongs to the technical field of functional material research, in particular to a method for synthesizing fluorescent nano-gold clusters modified with bovine serum albumin and using the synthesized functionalized nano-gold clusters as fluorescent probes to detect Cu 2+ Methods. Background technique [0002] With the rapid rise and development of various industries such as smelting, chemical industry, mining, electroplating, and medicine, the problem of heavy metal pollution in my country is becoming more and more serious. Copper is widely used in mechanical processing, mining and smelting, and electrical and electronic industries. Although copper is an indispensable micronutrient element for human health, it plays an extremely important role in the blood, nervous center and immune system. However, excessive intake of copper in the human body can also cause a series of poisoning phenomena such as nausea, vomiting and acute hemolysis. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N21/6428G01N2021/6432
Inventor 蔡会武王行行
Owner XIAN UNIV OF SCI & TECH
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