Specific fluorescent probe for identifying hydrazine and application thereof

A fluorescent probe, specific technology, applied in fluorescence/phosphorescence, luminescent materials, material analysis through optical means, etc., can solve environmental pollution and other problems, achieve simple and easy synthesis process, cheap and easy-to-obtain raw materials, good fluorescence The effect of the characteristic

Inactive Publication Date: 2014-07-16
CHANGSHU RES INST OF DALIAN UNIV OF TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrazine is also highly toxic, and it is easy to cause serious pollution to the environment during manufacture, use, transportation and disposal.

Method used

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  • Specific fluorescent probe for identifying hydrazine and application thereof
  • Specific fluorescent probe for identifying hydrazine and application thereof
  • Specific fluorescent probe for identifying hydrazine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The chemical synthesis of embodiment 1 fluorescent probe

[0021]

[0022] Put 1 mmol 4-trifluoromethyl-7-aminocoumarin, 1.1 mmol phthalic anhydride, 2 mmol sodium acetate in a 25 ml two-necked bottle, add 10 ml acetic acid, reflux at 110°C for 48 hours, and depressurize The solvent was distilled off, the residual solid was washed with water, and filtered to obtain 112 mg of a light yellow solid as a fluorescent probe. For the characterization results of the fluorescent probe, see figure 1 , figure 2 with image 3 .

Embodiment 2

[0023] Embodiment 2 For the selectivity of different substances

[0024] (1) Prepare 99 μl metabolic reaction system in advance, including PBS buffer (10 mM) at pH 7.4: DMSO (volume ratio 1:9), fluoride ion (100 μM), chloride ion (50 μM), bromide ion (100 μM), Iodide ion (100μM), sodium ion (100μM), potassium ion (100μM), calcium ion (100μM), magnesium ion (100μM), nitrate ion (100μM), hydrazine (100μM)

[0025] (2) Add 1 μl probe with a final concentration of 10 μM to the reaction system to initiate the reaction;

[0026] (3) After 40 min, perform fluorescence detection; calculate the fluorescence intensity in each system (see Figure 4 ).

Embodiment 3

[0027] Embodiment 3 linear relationship with hydrazine concentration

[0028] (1) Prepare 99 μl metabolic reaction system in advance, including PBS buffer (10 mM) at pH 7.4: DMSO (volume ratio 1:1), hydrazine (0-200 μM), and react at 37°C for 40 minutes;

[0029] (2) Add 1 μl probe with a final concentration of 10 μM to the reaction system to initiate the reaction;

[0030] (3) After 40 min, perform fluorescence detection; calculate the fluorescence intensity in each system, and establish a standard curve of fluorescence intensity and hydrazine concentration. The standard curve is y = 762.68x + 1845.5, R2 = 0.9973, where y represents the fluorescence intensity at 496 nm The ratio of the fluorescence intensity at 450 nm, x represents the concentration of hydrazine (the linear relationship between the concentration of hydrazine is satisfied between 1 uM and 150 uM) (see Figure 5 ).

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Abstract

The invention discloses a specific fluorescent probe for identifying hydrazine and an application thereof, belonging to the field of fine chemical engineering. The fluorescent probe is a 4-trifluoromethyl-7-aminocoumarin derivative, and is prepared by steps of putting 4-trifluoromethyl-7-aminocoumarin, phthalic anhydride and sodium acetate into a reaction bottle in proportion, and performing backflow by adding acetic acid. The fluorescent probe and the corresponding hydrazine content detection process cannot be interfered by biological system matrixes and impurities, so the fluorescent probe can be used for quantitative measurement of the hydrazine content in various biological systems. The probe has high specificity, and can be subjected to hydrolysis after specifically reacting with hydrazine; a hydrolysate has good fluorescence characteristics. Raw materials are cheap and easily available, can be obtained through chemical synthesis, and synthesis process is simple and feasible; the probe has high sensitivity, is suitable for detecting the hydrazine content in cells, and can be used for measuring the hydrazine content by drawing a standard curve. The probe is a ratio type probe, and effectively avoids the influences of non-uniform probe distribution, environmental factors, actuators and the like on the measurement results.

Description

technical field [0001] The invention relates to a specific fluorescent probe for identifying hydrazine and its application, belonging to the field of fine chemical industry. Background technique [0002] The chemical formula for hydrazine is N 2 h 4 , is an important industrial raw material, easily soluble in water to form hydrazine hydrate. Hydrazine is a reducing agent that plays an important role in chemical industry, pharmaceutical industry and agricultural industry, and can be used to prepare various industrial products including catalysts, preservatives, textile dyes and pharmaceutical intermediates. Hydrazine is also an important commercial energy fuel, widely used in rocket booster systems and missile systems. However, hydrazine is also highly toxic, and it is easy to cause serious pollution to the environment during its manufacture, use, transportation and disposal. In fact, hydrazine is classified as a human carcinogen by the U.S. Environmental Protection Agenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D405/04C09K11/06G01N21/64
CPCC07D405/04C09K11/06C09K2211/1029C09K2211/1088G01N21/6428
Inventor 崔京南冯磊秦增增
Owner CHANGSHU RES INST OF DALIAN UNIV OF TECH CO LTD
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