Tumor-targeting functionalized quantum dot and preparation method thereof

Abstract

The invention, which belongs to the field of biochemistry, relates to a tumor-targeting functionalized quantum dot and a preparation method thereof. The functionalized quantum dot consists of folic acid, cyclodextrin and quantum dot, and part of folic acid molecules are contained in the hydrophobic cavity of the cyclodextrin to form folic acid-cyclodextrin inclusion compound, which is then combined with the surface of the quantum dot. In the process of preparing the functionalized quantum dot, the folic acid is first combined with the Gamma-cyclodextrin to form the inclusion compound, and the Gamma-cyclodextrin-folic acid inclusion compound is then modified on the surface of the quantum dot, so that the dual-function nano material with excellent fluorescent characteristic and tumor-targeting property is produced. Since the Gamma-cyclodextrin is used to include the folic acid, the water-solubility and dispersibility of the folic acid are improved. The folic acid and the quantum dot are connected together by an effective method, and the produced fluorescent probe can be used for biological research on tumor cells. The preparation method is simple, the cost is low, and the screening of a variety of tumor cells and an in-vivo experiment on mice indicate that the material is characterized by low toxicity, high selectivity and the like.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a functionalized quantum dot for specifically recognizing tumor cells and a preparation method thereof. Background technique [0002] Tumor is the number one disease of human beings. The early diagnosis and treatment of tumors is an important part of human health. At present, the research on the pathogenesis and treatment methods of tumors has become the focus of attention in the medical field. higher and higher requirements. Tumor-targeted probe molecules are an important means of researching and treating tumors in recent years. [0003] Folic acid is an important substance for preparing tumor-targeting probe molecules. The folate receptor is a glycoprotein receptor that is overexpressed in many tumor cells and barely expressed in the vast majority of normal tissues. Folate has a high affinity for folate receptors, so this feature can be used as a targeting ligand for ...

AI Technical Summary

Problems solved by technology

However, since the folic acid molecule itself is insoluble in water, it cannot be directly connected to the quantum dots. How to connect folic acid to the quantum dots while maintaining the good affinity and specific binding of folic acid and tumor folate receptors is the current research. a puzzle on

Existing technologies, such as Chinese patent applications with application numbers 200610098270.6 and 200810050803.2, both disclose improved tumor-targeting functionalized quantum dots with folic acid as a ligand. These technologies mostly use polymer compounds as mediators to make folic acid and Quantum dots can be combined, but the nanocomposite particles produced in this way are relatively large, and the effective group part of the folic acid molecule as a receptor ligand is easily affected by polymer compounds, and the tumor targeting is not strong, and the cell penetration weaker

[0006] In addition, the tumor-targeting quantum dots in the prior art also have problems such as wide emission spectrum, uneven size distribution, and low fluorescence quantum yield.

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