Fluorescence immunochromatography test strip prepared by taking aggregation-induced emission fluorescent microsphere as beacon carrier

A technology of aggregation-induced luminescence and fluorescence immunochromatography, which is applied in the fields of medical testing and food safety testing, can solve the problems of preparing fluorescent microspheres without tetramethyl tetrastyrylate material, and achieves good fluorescence characteristics and detection sensitivity. High, high-capacity effects

Active Publication Date: 2019-04-16
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above situation, the inventor has researched and developed a kind of fluorescent microspheres prepared by using the aggregation-induced luminescent material tetramethyl phenylacrylate (TCBPE). At present, there is no relevant report on the preparation and application of tetramethyl styrene acid tetramethyl styrene in fluorescent microspheres.

Method used

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  • Fluorescence immunochromatography test strip prepared by taking aggregation-induced emission fluorescent microsphere as beacon carrier
  • Fluorescence immunochromatography test strip prepared by taking aggregation-induced emission fluorescent microsphere as beacon carrier
  • Fluorescence immunochromatography test strip prepared by taking aggregation-induced emission fluorescent microsphere as beacon carrier

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Preparation of aggregation-induced luminescence fluorescent microspheres (preparation of TCBPE dyes)

[0037] 1. Prepare the dye preparation solution: Weigh TCBPE (the synthesis method of TCBPE comes from the document Rigidifying Fluorescent Linkers by Metal-Organic Framework Formation for Fluorescence Blue Shift and Quantum Yield Enhancement Journal of the American Chemical Society 2014 136 (23), reported in 8269-8276), PMMA and PMAO is dissolved in chloroform, so that the final concentrations of TCBPE, PMMA, and PMAO dissolved in chloroform are 30, 50, and 25 mg / mL respectively;

[0038] 2. Preparation of microemulsion: Take 2400 μL of the above prefabricated solution, disperse it in 12 mL of 0.25% SDS solution, and perform ultrasonic emulsification with an ultrasonic breaker for 3 minutes under ice bath conditions, and the emulsification power is 600W;

[0039] 3. Solidify into balls: put the above emulsion in a rotary evaporator, and evaporate until there...

Embodiment 2

[0040] Example 2: Preparation of a competitive immunochromatographic test strip for the detection of 25-hydroxyvitamin D (25-OH-VD) prepared using aggregation-induced luminescence fluorescent microspheres as a beacon carrier

[0041] 1. Preparation process of immunochromatographic test strips

[0042] 1. Preparation of nitrocellulose membrane;

[0043] (1) Preparation of 25-OH-VD artificial antigen (25-OH-VD-OVA):

[0044] The coupling method is mixed acid anhydride method, the coupling protein is ovalbumin (OVA), and the coupling ratio is 1:80. After coupling, dialysis purification is used to obtain 25-OH-VD-OVA.

[0045] (2) Preparation of detection line and quality control line:

[0046] 25-OH-VD-OVA conjugates and anti-mouse antibodies were coated onto nitrocellulose membranes: dilute the concentration of 25-OH-VD-OVA conjugates with 0.01M PBS (phosphate buffer) at pH 7.5 The concentration of the diluted anti-mouse antibody is 1mg / mL, and the resulting solution is spray...

Embodiment 3

[0061] Example 3: Preparation of a competitive immunochromatographic test strip for the detection of zearalenone prepared using aggregation-induced luminescent fluorescent microspheres as a beacon carrier

[0062] 1. Preparation process of immunochromatographic test strips

[0063] 1. Preparation of nitrocellulose membrane;

[0064] (1) Preparation of ZEN artificial antigen (ZEN-BSA):

[0065] The coupling method is the mixed acid anhydride method, the coupling protein is bovine serum albumin (BSA), and the coupling ratio is 1:100. After coupling, it is purified by dialysis to obtain ZEN-BSA.

[0066] (2) Preparation of detection line and quality control line:

[0067] ZEN-BSA conjugates and anti-mouse antibodies were coated onto nitrocellulose membranes: Dilute the ZEN-BSA conjugates to a concentration of 6 mg / mL with 0.01M pH 7.0 PBS (phosphate buffered saline), and the resulting solution was in Spray on the membrane as a test line; the concentration of the diluted anti-m...

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Abstract

The invention provides a fluorescence immunochromatography test strip prepared by taking an aggregation-induced emission fluorescent microsphere as a beacon carrier. Filter paper, a sample cushion, anAIEFM (Aggregation-Induced Emission Fluorescent Microsphere) compound glass fiber cushion, a nitrocellulose membrane sprayed with a detection line and a quality control line, and water adsorption paper are overlapped and pasted to a bottom plate in sequence to prepare the fluorescence immunochromatography test strip, the fluorescence immunochromatography test strip is used for quantitatively detecting the concentration of an object to be tested in a sample, and the prepared test strip has the characteristics of being high in detection sensitivity.

Description

technical field [0001] The invention belongs to the fields of medical testing and food safety testing, and specifically relates to a fluorescent immunochromatographic test strip prepared by using aggregation-induced luminescent fluorescent microspheres as a beacon carrier to quantitatively detect the concentration of analytes in samples. Background technique [0002] Immunochromatography is a detection method based on antigen-antibody specific reaction. It has the advantages of fast detection speed, good specificity, simple operation and low cost. Compared with other methods, it can better meet the needs of on-site mass detection. Therefore, In recent years, it has developed rapidly and is widely used in food safety, medical inspection and environmental pollutant monitoring and other fields. Among them, colloidal gold immunochromatographic test strips are the most widely used immunochromatographic products, but their sensitivity is poor and they are easily interfered by matr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/553
CPCG01N33/533G01N33/553
Inventor 赖卫华熊勇华魏华陈媛章钢刚许恒毅喻志标彭娟
Owner NANCHANG UNIV
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