Method for producing fluorescent/colorimetric dual-pattern paper chips for detection of cancer cell and cell surface polysaccharide

A cell surface and paper chip technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve the problems of infeasible dynamic characterization of polysaccharide expression, complex sample pretreatment, strong destructiveness, etc., and achieve improvement Sensitivity, signal enhancement, and effect of increasing specific surface area

Inactive Publication Date: 2016-11-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, many methods (such as high performance liquid chromatography, mass spectrometry, nuclear magnetic resonance, etc.) are applied to the analysis of polysaccharides, but these methods are very destructive, making the dynamic characterization of polysaccharide expression on the surface of living cells infeasible
In addition, these methods are time-consuming, requiring complex sample preparation and expensive instrumentation

Method used

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  • Method for producing fluorescent/colorimetric dual-pattern paper chips for detection of cancer cell and cell surface polysaccharide
  • Method for producing fluorescent/colorimetric dual-pattern paper chips for detection of cancer cell and cell surface polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Application of fluorescence / colorimetric dual-mode paper chip in MCF-7 cells:

[0038] (1) Design the hydrophobic wax printing area and hydrophilic working area of ​​the fluorescent layer and colorimetric layer of the paper chip on the computer, and print the pattern on A4 size chromatographic paper;

[0039](2) Grow gold nanorods and porous silver layer on the working area of ​​the fluorescent layer of the paper chip obtained in step (1): Measure 160 mL of secondary water into a three-neck flask, heat to 90 ºC, then add 1.6 mL of gold chloride acid, heated to 96 ºC in a water bath, reacted for 1 min, immediately added 5.6 mL of 1% sodium citrate, and stirred for 15 min until the solution turned wine red; took 20 µL of gold seeds and added dropwise to the working area of ​​the fluorescent layer, at room temperature Leave it for 45 minutes;

[0040] Take 20.0 µL of 1% chloroauric acid and freshly prepared 200 mM hydroxylamine hydrochloride dropwise onto the paper with g...

Embodiment 2

[0048] Application of fluorescence / colorimetric dual-mode paper chip in the detection of polysaccharides on the surface of MCF-7 cells:

[0049] (1) Design the hydrophobic wax printing area and hydrophilic working area of ​​the fluorescent layer and colorimetric layer of the paper chip on the computer, and print the pattern on A4 size chromatographic paper;

[0050] (2) Grow gold nanorods and porous silver layer on the working area of ​​the fluorescent layer of the paper chip obtained in step (1): Measure 160 mL of secondary water into a three-neck flask, heat to 90 ºC, then add 1.6 mL of gold chloride acid, heated to 96 ºC in a water bath, reacted for 1 min, immediately added 5.6 mL of 1% sodium citrate, and stirred for 15 min until the solution turned wine red; took 20 µL of gold seeds and added dropwise to the working area of ​​the fluorescent layer, at room temperature Leave it for 45 minutes;

[0051] Take 20.0 µL of 1% chloroauric acid and freshly prepared 200 mM hydrox...

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Abstract

The invention discloses a method for producing fluorescent/colorimetric dual-pattern paper chips for detection of cancer cell and cell surface polysaccharide. According to the method, the paper chips are produced by a wax printing technology, gold nanorods and porous silver and aptamers are grown in a fluorescent layer work area, then cancer cell capture is carried out, a multi-branched hybridization technology is used for amplification of signal molecule, and accurate measurement of the cancer cells can be realized, the produced paper chips are folded, a chromogenic solution is dropped in a reaction area, and thereby visualization pre-measurement of the cancer cells can be realized. A polysaccharide inhibitor is added, and dynamic symptom of cell surface polysaccharide is realized.

Description

technical field [0001] The present invention relates to the technical field of low-cost, easy-to-carry, and visualized analysis and detection of cancer cells and cell surface polysaccharides, more specifically, the preparation of a fluorescence / colorimetric dual-mode sensor capable of detecting cancer cells and cell surface polysaccharides. It involves the use of multi-branch hybridization chain signal amplification technology. Background technique [0002] In recent years, cancer has become the number one killer of human beings, threatening human health greatly. The metastasis and spread of cancer cells is an important reason for the death of cancer. Therefore, early detection and timely treatment are the fundamental methods to overcome cancer. Polysaccharides are an important structure on the cell surface, and polysaccharides are distributed in high density on the surface and inside of cell membranes through covalent interactions with proteins or lipids. The dynamic chang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/6402G01N21/6486G01N21/78
Inventor 葛慎光梁琳琳于京华李丽兰飞飞任娜刘海云张彦颜梅
Owner UNIV OF JINAN
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