A fluorescent sensor, its preparation method and use

A fluorescent sensor and application technology, applied in the field of biosensors, to achieve the effects of simple operation, low detection limit and low cost

Active Publication Date: 2018-05-08
ANHUI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this area also lacks a kind of accurate sensor to carry out above-mentioned detection work

Method used

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  • A fluorescent sensor, its preparation method and use
  • A fluorescent sensor, its preparation method and use
  • A fluorescent sensor, its preparation method and use

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Embodiment Construction

[0029] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment of various embodiments of the present invention.

[0030] In a preferred embodiment, a method for preparing manganese dioxide nanosheets and a super-sandwich-based fluorescence sensor includes the following steps:

[0031] (1) DNA sequence to be purchased (HP 1 , HP 2 , HelperDNA) dissolved in PBS (pH 7.4) buffer solution, and stored at low temperature for later use, before using HP 1 , HP 2 Heat at 80-90°C for 10 minutes and then naturally cool to room temperature and incubate for a period of time;

[0032] (2) For the substances used, such as manganese dioxide nanosheets, coralyne, heparin, and SG are configured to corresponding concentrations;

[0033] (3) Put HP 1 ,HP 2 ,helperDNA,coralyne,SG,manganese dioxide nanosheets were added to the solution and incubated at 45℃ for 10 minutes;

[0034] (4) The concentration of heparin is different, the amount ...

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Abstract

The present invention relates to a kind of fluorescence sensor, and its preparation method and application, specifically relate to the biological fluorescence sensor of manganese dioxide nano-sheet and super-sandwich base, use manganese dioxide nano-sheet to quench fluorescence, and single-stranded DNA can be adsorbed on the carbon dioxide The properties of manganese nanosheets and dsDNA and other secondary structures of DNA can be used in the detection of heparin away from the manganese dioxide nanosheets. It includes the following steps: (1) dissolving the DNA sequence (HP1, HP2, helperDNA) in a buffer solution, and saving it for later use; (2) configuring the substances used to a corresponding concentration; (3) dissolving the HP1, HP2, helperDNA and the used Substances are added to the solution for incubation. The preparation method of the bioluminescent sensor uses unlabeled DNA, which is simple to operate and low in cost, and avoids any chemical labeling and modification. The results show that the sensor has a satisfactory detection result for heparin, and has simple operation, high sensitivity, Features low detection limit.

Description

Technical field [0001] The invention belongs to the technical field of biosensors based on two-dimensional nanomaterial manganese dioxide nanosheets, and specifically relates to the construction of a fluorescence sensor constructed based on two-dimensional nanomaterials to quench fluorescence and DNA-initiated super-sandwich to trigger luminescence signals for heparin Detection. Background technique [0002] The 21st century is the century of life sciences. The study of DNA is an extremely important aspect of life science research. The research of DNA biosensors has become a hot spot. Various methods are currently used to detect biomolecules. The emergence of two-dimensional nanomaterials has injected strong vitality into DNA-based sensors. The combination of two-dimensional nanomaterials and DNA-based sensors is a product of the times. Two-dimensional manganese dioxide nanosheets have been studied due to their good water solubility, excellent biocompatibility, and easy modifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 王广凤江红
Owner ANHUI NORMAL UNIV
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