59results about How to "Meet the technical requirements of safety testing" patented technology

The invention discloses a method for measuring indazole flusulfamide residue amount. The method comprises the following steps: homogenizing and extracting residual indazole flusulfamide in a sample by using acetonitrile or 1 percent of acetic acid-acetonitrile, dispersing and purifying the extracting solution through an ethidene diamine-N-propoxysilane (PSA) and octadecyl silane bonded phase (C18) matrix, performing liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection, establishing a corrected standard curve by adopting dilution standards of a blank matrix solution, and quantifying through an external standard method. According to the method, the average recovery rate is 84.2-91.4 percent, the average relative standard deviation (RSD) is 3.7-6.6 percent, the detection limit is 1.53mu g/kg, and the method has the advantages of simplicity and convenience in operation, high rapidness, high accuracy, high sensitivity and high repeatability. The technical requirements on the safety detection of corresponding products in European Union, USA, Korea, Japan, Taiwan and other countries can be met, and powerful technical support is provided for guaranteeing the food safety of people in China and the export abroad trade health development.

The invention discloses a method for measuring prothioconazole residue in food. The method includes following steps: S1, extracting; S2, purifying; S3, preparing a matrix standard working solution; S4, using a liquid chromatograph-series ultraviolet detector for measuring, including: (1), qualitative measuring; (2), quantitative measuring. A chromatographic column of liquid chromatograph is a hydrophilic chromatographic column, and a mobile phase measured by liquid chromatograph is a mixture of an acetonitrile solution and a mixed solution according to a volume ratio of 45:55. By the method, matrix interference during prothioconazole residue detection in food can be lowered effectively; the pretreatment method can be combined with HPLC-UV to be applied in qualitative confirmation and quantitative detection of prothioconazole in the food. The method has the advantages of being simple and convenient in operation, quick, accurate and high in sensitivity and repeatability and is a supplement to and an improvement on existing QuEChERS method. Technical requirements on safety detection of corresponding products can be met completely, and powerful technical support is about to be providedfor ensuring food safety and sound development of foreign export trade.

The invention discloses a method for simultaneously detecting residual quantities of oxamyl and oxamyl oxime in vegetable/fruits. The method comprises the following steps: (1) extraction: vegetable/fruit samples to be detected are taken, acetonitrile and NaCl are added, and homogenizing and centrifugation are carried out; (2) purification: an upper layer acetonitrile phase is taken and placed in a centrifuge tube containing a matrix solid-phase dispersion extractant, centrifugation is carried out, supernatant is taken, drying is carried out with nitrogen, volumetric flask preparation is carried out for a mobile phase, a 0.22[mu]m organic filter membrane is used for filtering, and a sample liquid is obtained; (3) determination and result calculation: HPLC-MS/MS is used for detecting the sample liquid, chromatographic peak area of oxamyl or oxamyl oxime is detected, the value is substituted into a standard curve, and content of oxamyl or oxamyl oxime in the sample liquid is obtained by calculation. The solid-phase dispersion extraction technology is used, the simple and rapid pre-treatment method is established, and detection limits of oxamyl and oxamyl oxime are 0.002mg/kg and 0.001 mg/kg respectively; the method has the advantages of simple operation, fast speed, accuracy, high sensitivity, and good repeatability.

The invention discloses a method for determining chlormequat and mepiquat chloride residue amount, belonging to the food safety analysis technology field. The determining method for the chlormequat and mepiquat chloride residue amount comprises the following steps: firstly, pre-processing a sample; secondly, using a liquid chromatography-tandem mass spectrometry (LC/MS-MS) method for determining the chlormequat and mepiquat chloride residue amount in the processed sample to be determined; thirdly, preparing a standard curve and a result judgment. The method for determining the chlormequat and mepiquat chloride residue amount has the advantages of constructing a simple and convenient sample pre-processing method, developing advanced chromatographic separation technology and constructing a determining method with fast speed and high sensitivity (the detection limit is less than 0.005mg/kg) through an advanced instrument. The method can satisfy the technique requirements of European Union, America and Japan for the relative product safety detection and provide a powerful technical support to the food safety security of our people and the health development of foreign export trade.

The invention discloses a determination method for the residual amount of metrafenone. The determination method comprises the following steps: homogenizing by using acetonitrile or 1% acetonitrile acetate to extract residual metrafenone in a sample; dispersing and purifying an extracting solution by ethylenediamine-N-propoxysilane (PSA) and an octadecyl silane bonded phase (C18) base material; carrying out liquid chromatogram-mass spectrum/mass spectrum (LC-MS/MS) detection; establishing a corrected standard curve by adopting a blank base material solution diluting standard; and quantifying by an external standard method. According to the method, the average recycling rate is 80.2%-94.9%; the average relative standard deviation (RSD) is 4.0%-6.8%; the detection limit is lower than 0.10 microgram/kilogram; the determination method has the advantages of simplicity and convenience in operation, rapidness, high sensitivity, good repeatability and accuracy in qualifying and quantifying. The technical requirements of corresponding product safety detection by countries including European Union, America, Korea, Japan and the like can be met and the powerful technical supports are provided for guaranteeing the food safety of people in China and the healthy development of export trades.

The invention discloses a detecting method for the residual quantity of flubendiamide in fruits and vegetables. The detecting method comprises the steps of homogenizing by using acetonitrile or an acetonitrile liquid containing 1% acetic acid to extract residual flubendiamide in a sample; dispersing by using a primary secondary amine (PSA) and octadecylsilane bonded-phase (C18) matrix to purify an extracting liquid; then, carrying out gas chromatography-negative chemical ionization-mass spectrum (GC-NCI-MS) detection; establishing a corrected standard curve by using a blank matrix liquid without a pesticide to be detected; and quantifying by using an external standard method. By using the detecting method, the average recovery rate is 93.2-103.1%, the average relative standard deviation (RSD) is 4.6-7.4%, and the detection limit is lower than 1.15mu g/kg; and the detecting method is simple and convenient to operate, rapid, high in sensitivity, good in repeatability and accurate in qualitation and quantitation. According to the detecting method, the technical requirements of America, Japan, European union and other countries on security detection of corresponding products can be met, and the powerful technical support can be provided for ensuring Chinese food safety and the health development of foreign export trade.

The invention discloses a GC-EI-MS (Gas Chromatography-Electron Ionization-Mass Spectrum) detecting method for the residual quantity of cyenopyrafen in fruits and vegetables. The GC-EI-MS detecting method comprises the steps of homogenizing by using acetonitrile or an acetonitrile liquid containing 1% acetic acid to extract residual cyenopyrafen in a sample; dispersing by using a primary secondary amine (PSA) and octadecylsilane bonded-phase (C18) matrix to purify an extracting liquid; then, carrying out GC-EI-MS detection; establishing a corrected standard curve by using a blank matrix liquid without a pesticide to be detected; and quantifying by using an external standard method. By using the GC-EI-MS detecting method, the average recovery rate is 87.2-92.6%, the average relative standard deviation (RSD) is 4.0-6.5%, and the detection limit is lower than 1.33mu g/kg; and the GC-EI-MS detecting method is simple and convenient to operate, rapid, high in sensitivity, good in repeatability and accurate in qualitation and quantitation. According to the GC-EI-MS detecting method, the technical requirements of Korea, Japan, European union and other countries on security detection of corresponding products can be met, and the powerful technical support can be provided for ensuring Chinese food safety and the health development of foreign export trade.

The invention discloses a GC-NCI-MS (gas chromatography-negative chemical ionization-mass spectrometry) determination method of residual amount of metrafenone in fruits and vegetables. The method comprises the following steps: homogenizing and extracting residual metrafenone in a sample with acetonitrile or an acetonitrile solution containing 1% of acetic acid, performing dispersion and purification on an extraction solution with a matrix of ethylenediamine-N-propylsilane (PSA) and octadecylsilane bonded phase (C18), then performing GC-NCI-MS detection, establishing a standard curve for correction by adopting a blank matrix solution without a pesticide to be detected, and quantifying by an external standard method. According to the method, the average recovery rate is 89.9%-94.9%, the average relative standard deviation (RSD) is 4.1%-6.2%, the detection limit is lower than 1.02mu g/kg, and the method has the advantages of simplicity and convenience in operation, high speed, high sensitivity, good repeatability and accurate qualitative and quantitative properties. According to the method, the technical requirements on safety detection of corresponding products in European Union, United States, South Korea, Japan and other countries can be met, and a powerful technical support is provided for ensuring food safety of people in China and health development of export trades.

The invention discloses a method for measuring the residual quantity of chlorantraniliprole in fruit and vegetable. The method comprises performing homogeneous extraction on chlorantraniliprole residue in a sample through acetonitrile or an acetonitrile solution containing 1% of acetic acid; performing dispersed purification on extracted solution through ethylenediamine-N-propyl silicane (PSA) and octadecyl silane bonded phase (C18) matrix; then performing gas chromatograph-negative chemical ionization-mass spectrometry (GC-NCI-MS) detection, establishing a calibration standard curve through blank matrix solution not containing pesticide to be measured, and performing quantitation through the external standard method. The method for measuring the residual quantity of the chlorantraniliprole in fruit and vegetable achieves an average recovery rate of 90.7-101.9%, an average relative standard deviation (RSD) of 5.4-9.6% and a detection limit lower than 0.77 mu g/kg, has the advantages of being convenient and rapid to operate, high in sensitivity and repeatability and accurate in quantitation and quantification, can meet the technical requirements of the corresponding product safety inspection of nations such as the U.S., Japan, the European Union and Canada, and provides powerful technical support for food safety of people and healthy development of export trade in China.

The invention discloses a GC-NCI-MS (Gas Chromatography-Negative Chemical Ionization-Mass Spectrum) detecting method for the residual quantity of cyenopyrafen in fruits and vegetables. The GC-NCI-MS detecting method comprises the steps of homogenizing by using acetonitrile or an acetonitrile liquid containing 1% acetic acid to extract residual cyenopyrafen in a sample; dispersing by using a primary secondary amine (PSA) and octadecylsilane bonded-phase (C18) matrix to purify an extracting liquid; then, carrying out GC-NCI-MS detection; establishing a corrected standard curve by using a blank matrix liquid without a pesticide to be detected; and quantifying by using an external standard method. By using the GC-NCI-MS detecting method, the average recovery rate is 86.3-94.3%, the average relative standard deviation (RSD) is 4.6-8.6%, and the detection limit is lower than 1.12mu g/kg; and the GC-NCI-MS detecting method is simple and convenient to operate, rapid, high in sensitivity, good in repeatability and accurate in qualitation and quantitation. According to the GC-NCI-MS detecting method, the technical requirements of Korea, Japan, European union and other countries on security detection of corresponding products can be met, and the powerful technical support can be provided for ensuring Chinese food safety and the health development of foreign export trade.

The invention discloses a GC-MS/MS method for determining fluxapyroxad residues in fruits and vegetables. According to the method, acetonitrile or an acetonitrile solution containing 1% acetic acid are adopted to homogeneously extract the residual fluxapyroxad in a sample, the extracting solution is subjected to matrix dispersion and purification of ethylenediamine-N-propyl silane (PSA) and an octadecyl silane bonded phase (C18), then gas chromatography-tandem mass spectrometry (GC-MS/MS) detection is performed, a blank matrix solution containing no pesticide to be detected is adopted to establish a standard working curve for correction, and quantification is performed by adopting an external standard method. The average recovery rate of the method is 86.0%-96.0%, the average relative standard deviation (RSD) of the method is 3.5%-7.1%, the detection limit of the method reaches 0.064 micrograms/kilogram or below, and the method has the advantages of being simple and convenient to operate, rapid, high in sensitivity, good in repeatability and accurate in qualititation and quantification, can meet the technical requirements for corresponding product safety detection of the United States, European Union, Japan and other countries and provides a powerful technical support for ensuring the people's food safety and healthy development of the abroad export trade of our country.

The invention discloses a GC-MS/MS (gas chromatography-tandem mass spectrometry) determining method of penthiopyrad residual quantity. The method is a method mainly used for determining the residual penthiopyrad content in agricultural products of complicated matrix food such as cereal grains and animal derived food. Acetonitrile or an acetonitrile solution containing one-percent acetic acid is used for performing homogeneous extraction of the residual penthiopyrad in samples; after C<18>/PSA (primary secondary amine) solid phase extraction column purification and concentration, GC-MS/MS detection is performed; a corrected standard work curve is built by using a blank matrix solution not containing pesticide to be detected; an external reference method is used for quantification. The GC-MS/MS determining method has the advantages that the average recovery rate is 86.4 percent to 95.3 percent; the average RSD (relative standard deviation) is 5.0 percent to 8.7 percent; the detection limit is lower than 0.25mug/kg; the operation is simple, convenient and fast; the impurity removal effect is good; the sensitivity is high; the characteristic performance is high; the repeatability is good; the qualitative and quantitative analysis is accurate. The technical requirements of countries such as America, European Union and Japan on corresponding product safety detection can be met; powerful technical support is provided for guaranteeing the food safety of Chinese people and healthy development of export abroad trade.

The invention discloses a method for determining penthiopyrad residue in fruits and vegetables through GC-MS/MS (gas chromatography-tandem mass spectrometry). Acetonitrile or an acetonitrile solution containing one-percent acetic acid is used for performing homogeneous extraction of residual penthiopyrad in samples; after an extracting solution is subjected to PSA(primary secondary amine)/C<18> (octadecylsilyl) matrix dispersed purification, GC-MS/MS detection is performed; a corrected standard work curve is built by using a blank matrix solution not containing pesticide to be detected; an external reference method is used for quantification. The method has the advantages that the average recovery rate is 92.2 percent to 101.3 percent; the average RSD (relative standard deviation) is 3.9 percent to 7.3 percent; the detection limit is lower than 0.26mug/kg; the operation is simple, convenient and fast; the sensitivity is high; the repeatability is good; the qualitative and quantitative analysis is accurate. The technical requirements of countries such as America, European Union and Japan on corresponding product safety detection can be met; powerful technical support is provided for guaranteeing the food safety of Chinese people and healthy development of export abroad trade.

The invention provides a method for detecting the residual quantity of prochloraz and metabolites thereof in cereal grains, which utilizes a gas chromatography-triple quadrupole mass spectrometer for testing and specifically comprises the following steps: preparing a matrix mixed standard series working solution, extracting a sample, purifying a sample solution and detecting the sample on a machine. According to the method, the influence of factors such as the type of an extraction solvent, the type and dosage selection of a dispersive solid-phase extraction agent and the like on the extraction efficiency is investigated, and gas chromatography conditions and a triple quadrupole mass spectrometer are subjected to condition optimization. The method is simple, convenient and efficient to operate, low in reagent consumption, capable of rapidly meeting the requirement for high-flux sample treatment, capable of meeting the pesticide residue detection requirements on precision, accuracy and sensitivity and suitable for detecting the residual quantity of the prochloraz and the metabolite thereof in the oil-containing cereal grains.

The invention discloses a GC (Gas Chromatograph)-EI (Electronic Impact Ionization)-MS (Mass Spectrometry) determining method of the residual quantity of fluxapyroxad. The GC-EI-MS determining method is mainly used for determining the content of residual fluxapyroxad in complex-matrix food and agricultural products of cereal grains, animal derived food and the like. The GC-EI-MS determining method comprises the following steps: homogeneously extracting the residual fluxapyroxad in a sample by using acetonitrile or an acetonitrile solution containing 1 percent of acetic acid; carrying out GC-EI-MS detection after purifying and concentrating by using a C18/PSA (Primary Secondary Amine) solid-phase extraction column; building a corrected standard work curve by adopting a blank-matrix solution which does not contain to-be-detected pesticide; quantifying through an external standard method. According to the GC-EI-MS determining method of the residual quantity of the fluxapyroxad, disclosed by the invention, the average recovery rate is 84.6 to 103.1 percent, the average RSD (Relative Standard Deviation) is 3.2 to 8.4 percent, and the detection limit is lower than 1.01 mug/kg; the GC-EI-MS determining method has the advantages that the operation is convenient and fast, the impurity removing effect is good, the sensitivity is high, the characteristic performance is strong, the repeatability is good, and qualitativeness and quantitativeness are precise; the technical requirements on safety detection of corresponding products of countries of America, EU (European Union), Japan and the like can be met, and a powerful technical support is provided for ensuring the safety of food for people in China and the healthy development of export trade.