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Method for measuring prothioconazole residue in food

A technology of prothioconazole and a determination method is applied in the field of determination of prothioconazole residues in food, and achieves the effects of high sensitivity, easy operation and reduced matrix interference

Inactive Publication Date: 2019-04-19
苏农(广德)生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

There is no simple and specific prothioconazole residue detection method in the prior art, so it is necessary to seek a simpler and more convenient analysis method

Method used

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  • Method for measuring prothioconazole residue in food
  • Method for measuring prothioconazole residue in food
  • Method for measuring prothioconazole residue in food

Examples

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Embodiment

[0052] Instruments and reagents used in this embodiment:

[0053] T18Basic homogenizer (IKA, Germany); CR21GⅢ high-speed refrigerated centrifuge (Hitachi, Japan); MS3 vortex mixer (IKA, Germany); 1200 HPLC-UV detector (Agilent, USA); PSA dispersion Solid-phase extraction agent (Agilent, the U.S.); C18 dispersed solid-phase extraction agent (Agilent, the U.S.);

[0054] Reagents: water (ultrapure water, wahhaha); acetonitrile (HPLC grade, Merke, Germany); ammonium formate (HPLC grade, CNW, Germany); formic acid (HPLC grade, CNW, Germany); ethyl benzoate (analytical grade , as an internal standard);

[0055] Prothioconazole standard substance: the purity is 99.0%, purchased from Dr-Ehrenstorfer Company in Germany.

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Abstract

The invention discloses a method for measuring prothioconazole residue in food. The method includes following steps: S1, extracting; S2, purifying; S3, preparing a matrix standard working solution; S4, using a liquid chromatograph-series ultraviolet detector for measuring, including: (1), qualitative measuring; (2), quantitative measuring. A chromatographic column of liquid chromatograph is a hydrophilic chromatographic column, and a mobile phase measured by liquid chromatograph is a mixture of an acetonitrile solution and a mixed solution according to a volume ratio of 45:55. By the method, matrix interference during prothioconazole residue detection in food can be lowered effectively; the pretreatment method can be combined with HPLC-UV to be applied in qualitative confirmation and quantitative detection of prothioconazole in the food. The method has the advantages of being simple and convenient in operation, quick, accurate and high in sensitivity and repeatability and is a supplement to and an improvement on existing QuEChERS method. Technical requirements on safety detection of corresponding products can be met completely, and powerful technical support is about to be providedfor ensuring food safety and sound development of foreign export trade.

Description

technical field [0001] The invention belongs to the technical field of determination of pesticide residues, in particular to a method for determination of prothioconazole residues in food. Background technique [0002] During the planting of fruits, vegetables, grains and other crops, fungal and bacterial diseases in farmland have an important impact on crop yield and quality. The use of fungicides in agricultural production reduces the loss of yield and quality of agricultural products caused by fungal and bacterial diseases, and obtains considerable economic benefits. However, with people's attention to food safety issues, the hazards of fungicide residues to human health have also attracted much attention. Such as fungicide prothioconazole (prothioconazole) chemical name 2-(2-(1-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl-1-2-dihydro-3 -1,2,4-triazole-3-thiol belongs to broad-spectrum triazolethione fungicides.The mechanism of action of prothioconazole is to i...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8845
Inventor 汪志勇刘天顺邹小翠乐观琼汤晓娟杨红艳
Owner 苏农(广德)生物科技有限公司
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