Method for measuring residual quantity of cycocel and mepiquat chloride

A method of determination, the technology of mepipector amine, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of no organic solvent pollution, fast detection speed and high sensitivity

Inactive Publication Date: 2008-12-10
PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
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  • Application Information

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Problems solved by technology

There is no report on the detection method using ultra-high pressure liquid chromatography-tandem mass spectrometry (UPLC / ESI-MS / MS)

Method used

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  • Method for measuring residual quantity of cycocel and mepiquat chloride
  • Method for measuring residual quantity of cycocel and mepiquat chloride
  • Method for measuring residual quantity of cycocel and mepiquat chloride

Examples

Experimental program
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Effect test

Embodiment 1

[0077] Example 1: UPLC / MS-MS method for rapid analysis and determination of chlormequat and chlormequat residues in tomato and tomato paste

[0078] 1. Preparation of standard curve: Accurately weigh 5.0g blank sample and accurately weigh 20mL of secondary water, vortex mix at 2500r / min or more for 1min, ultrasonically extract for 20-30min, centrifuge at 8500-10000r / min for 10min, take the supernatant through the aperture The matrix solution to be tested was prepared using a filter membrane of 0.2 μm; use this solution to dilute the standard intermediate solution to 1.0, 2.0, 5.0, 10.0, 15.0, 20.0, 50.0 ng / mL, but make a standard working curve at the origin to obtain Regression analysis was performed on the response value (peak area) Y and the concentration x. The regression curves of chlormequat and mepiquat are Y=81.7x-47.6 and Y=107.0x+8.2 respectively, and the linear correlation coefficients are 0.995 and 0.999 respectively. Their standard working curves are as follows fi...

example 2

[0087] Example 2: UPLC / MS-MS method for rapid analysis and determination of chlormequat and metramine residues in apples

[0088] The preparation of the standard curve, the sample pretreatment steps and the addition test method are all the same as in Example 1, the established standard working curve equation is 84.3383x+5.09253, and the correlation coefficient is 0.9989. The results of the addition recovery test are shown in Table 3. Similarly, this example shows that the method is simple, sensitive and reliable.

[0089] Table 3: The recovery rate and precision determination results of apple matrix (n=3)

[0090]

example 3

[0091] Example 3: UPLC / MS-MS method for rapid analysis and determination of chlormequat and mepiquat residues in watermelon

[0092] The preparation of the standard curve, the sample pretreatment steps and the addition test method are all the same as in Example 1, and the established standard working curve equation is 168.01x+13.9386, and the correlation coefficient is 0.9979. Addition recovery test results are shown in Table 4. Similarly, this example shows that the method is simple, sensitive and reliable.

[0093] Table 4: The recovery rate and precision determination results of watermelon matrix (n=3)

[0094]

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Abstract

The invention discloses a method for determining chlormequat and mepiquat chloride residue amount, belonging to the food safety analysis technology field. The determining method for the chlormequat and mepiquat chloride residue amount comprises the following steps: firstly, pre-processing a sample; secondly, using a liquid chromatography-tandem mass spectrometry (LC/MS-MS) method for determining the chlormequat and mepiquat chloride residue amount in the processed sample to be determined; thirdly, preparing a standard curve and a result judgment. The method for determining the chlormequat and mepiquat chloride residue amount has the advantages of constructing a simple and convenient sample pre-processing method, developing advanced chromatographic separation technology and constructing a determining method with fast speed and high sensitivity (the detection limit is less than 0.005mg/kg) through an advanced instrument. The method can satisfy the technique requirements of European Union, America and Japan for the relative product safety detection and provide a powerful technical support to the food safety security of our people and the health development of foreign export trade.

Description

technical field [0001] The invention relates to a method for determining the residual amount of chlormequat and mepiquat, more specifically, it uses high-performance liquid chromatography-tandem mass spectrometry (LC / MS / MS) to quickly qualitatively and quantitatively determine vegetables, fruits and their products, and soil 1. The method for the content of chlormequat and mepiquat remaining in the fertilizer belongs to the technical field of food safety inspection. Background technique [0002] With the improvement of my country's quality requirements for agricultural products and the substantial increase in foreign trade volume, the requirements for the detection of pesticide residues are also more stringent, and the tests and challenges of international food safety inspections and foreign technical barriers are becoming more and more severe. Moreover, the larger the export product, the greater the possibility of suffering from technical barriers and trade sanctions. Repre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 王金花徐超一陈广全卢晓宇张朝晖黄梅吴瑑
Owner PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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