Insect cell serum-free culture medium and preparation process thereof

A technology of serum-free medium and insect cells, which is applied in the direction of cell culture active agent, culture process, animal cells, etc., can solve the problems of high cost of medium, unfavorable insect cell culture, etc., and achieve simple preparation process and good commercialization prospects , the effect of clear components

Pending Publication Date: 2019-12-13
广州今成生物科技有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing serum-free medium needs to add a variety of small molecule additives (such as recombinant proteins) or high-concentration vitamins, which makes the cost of the medium too high and is not conducive to large-scale insect cell culture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Insect cell serum-free culture medium and preparation process thereof
  • Insect cell serum-free culture medium and preparation process thereof
  • Insect cell serum-free culture medium and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation process of the insect cell serum-free medium provided in this embodiment comprises the following steps:

[0036] (1) Add each inorganic salt component in Table 1 into 20mL deionized water for dissolution to obtain an inorganic salt solution;

[0037] (2) Add each vitamin composition in Table 2 to 20mL deionized water and dissolve to obtain a vitamin solution;

[0038] (3) Dissolving each amino acid component in Table 3 in 20 mL deionized water to obtain an amino acid solution;

[0039] (4) Add the obtained inorganic salt solution, vitamin solution, and amino acid solution into a beaker containing 800mL of deionized water. After stirring evenly, carbohydrates, protein hydrolyzate, yeast powder, and fat The quality emulsifier was added into the beaker in turn, stirred evenly, and the volume was set to 1000mL to obtain the insect cell serum-free medium.

[0040] Table 1 Inorganic salt components and their contents in serum-free medium of insect cells

[...

Embodiment 2

[0051] The preparation process of the insect cell serum-free medium provided in this embodiment comprises the following steps:

[0052] (1) Add each inorganic salt component in Table 5 to dissolve in 20mL deionized water to obtain an inorganic salt solution;

[0053] (2) Dissolving each vitamin composition in Table 6 in 20mL deionized water to obtain a vitamin solution;

[0054] (3) Dissolving each amino acid component in Table 7 in 20 mL deionized water to obtain an amino acid solution;

[0055] (4) Add the obtained inorganic salt solution, vitamin solution, and amino acid solution into a beaker containing 800mL deionized water. After stirring evenly, carbohydrates, protein hydrolyzate, yeast powder, fat The quality emulsifier was added into the beaker in turn, stirred evenly, and the volume was set to 1000mL to obtain the insect cell serum-free medium.

[0056] Table 5 Components and contents of each inorganic salt in serum-free medium of insect cells

[0057]

[0058]...

Embodiment 3

[0067] The preparation process of the insect cell serum-free medium provided in this embodiment comprises the following steps:

[0068] (1) Dissolving each inorganic salt component in Table 9 in 20 mL deionized water to obtain an inorganic salt solution;

[0069] (2) Dissolving each vitamin composition in Table 10 in 20mL deionized water to obtain a vitamin solution;

[0070] (3) Dissolve each amino acid component in Table 11 in 20 mL of deionized water to obtain an amino acid solution;

[0071] (4) Add the obtained inorganic salt solution, vitamin solution, and amino acid solution into a beaker containing 800 mL of deionized water, and after stirring evenly, add the carbohydrates, protein hydrolyzate, yeast powder, fat The quality emulsifier was added into the beaker in turn, stirred evenly, and the volume was set to 1000mL to obtain the insect cell serum-free medium.

[0072] Table 9 Inorganic salt components and their contents in the serum-free medium of insect cells

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an insect cell serum-free culture medium and a preparation process thereof. The insect cell serum-free culture medium comprises 11-16 g / L of amino acid, 5-15 g / L of carbohydrates, 7-12 g / L of inorganic salt, 0.01-0.2 g / L of vitamin, 3-10 g / L of a protein hydrolysate, 1-5 g / L of yeast powder and 15-35 g / L of a lipid emulsifier. The insect cell serum-free medium provided by the invention is clear in components, low in vitamin content, relatively low in cost and simple in preparation process, is beneficial to large-scale culture of insect cells, can greatly improve the insect cell culture efficiency, and has a good commercial prospect.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an insect cell serum-free medium and a preparation process thereof. Background technique [0002] Cell culture is the propagation and cultivation of cells in vitro, which is an indispensable technology in the field of biological research. Traditional cell culture medium is mainly prepared by adding an appropriate amount of serum or tissue extract to the basic medium. Due to the limitation of animal serum extraction technology, the source of serum-containing medium is difficult and expensive, and the quality is unstable. , large batch-to-batch variation and poor repeatability, which brings difficulties to the standardization of cell culture. Due to the many problems of traditional cell serum medium, more and more researchers are committed to developing serum-free medium. [0003] In recent years, the serum-free culture technology of cells has developed rapidly, and multiple...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
CPCC12N5/0601C12N2500/32C12N2500/38C12N2500/12C12N2500/14C12N2500/16C12N2500/20C12N2500/22C12N2500/24C12N2500/10C12N2500/90C12N2501/999
Inventor 成浩
Owner 广州今成生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap