Gene chip for detection of cerebrospinal fluid pathogens

A technology of gene chips and pathogenic bacteria, applied in the field of gene chips in the field of biotechnology, can solve the problems of complicated operation, high cost, and high false positive rate

Inactive Publication Date: 2015-11-18
GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In the past, the PCR method was used to detect Sp, E.coli, Hi and Sa and other pathogenic bacteria, and the sensitivity and specificity were higher than those of CSF culture, which provided a basis for etiological diagnosis, but due to high cost, complicated operation, and false positive results High rate and other shortcomings, and was stopped by the national administrative department

Method used

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  • Gene chip for detection of cerebrospinal fluid pathogens
  • Gene chip for detection of cerebrospinal fluid pathogens
  • Gene chip for detection of cerebrospinal fluid pathogens

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Experimental program
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Embodiment Construction

[0042] Step 1: Design and preparation of detection probes:

[0043] The common pathogens of suppurative meningitis encephalitis are listed in the following table:

[0044]

[0045] Use the pathogenic bacteria in the above table from the NCBI Taxonomy database to find the TaxonomyID information of each microorganism in the database, etc.

[0046] Use bioinformatics means to retrieve the 16SrRNA sequence of each pathogen, and use relevant bioinformatics software (DNAStar and other software) to design oligonucleotide probes for each pathogen to be detected based on these sequences, and design 7 kinds of pathogens to be detected And optimized 153 probes, these probes covered most of the common subtypes of these 7 bacteria.

[0047] Crystal core Gene chip spotting solution and water, prepare oligonucleotide probes into a specific concentration spotting solution, then transfer to the corresponding position of the orifice plate according to the dot array pattern, and seal it wi...

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Abstract

Belonging to the field of gene chips, the invention relates to a gene chip for detection of cerebrospinal fluid pathogens. The gene chip provided by the invention includes a substrate and probes, and the probes are fixed on the substrate. Specifically, the probes are nucleotide sequences as shown in SEQ ID NO.1-153, and the substrate is one of a glass slide, a silicon chip, a film and a high polymer material. The gene chip provided by the invention has the advantages of simple operation step, high detection specificity, good stability, short time and low cost, and can realize rapid, low cost, large flux and automatic detection of the species of pathogenic bacteria in cerebrospinal fluid samples of purulent meningitis patients, thus providing help for diagnosis of purulent meningitis.

Description

Technical field: [0001] The invention relates to a gene chip in the field of biotechnology, in particular to a gene chip for detecting pathogenic bacteria in cerebrospinal fluid. Background technique: [0002] Suppurative meningitis has an incidence rate of 4 / 100,000 to 6 / 100,000 adults in developed countries. It is a disease that seriously endangers human health. Although the clinical application of antibiotics has cured many cases, this The morbidity and fatality rates of the disease remain unacceptably high worldwide. According to recent reports, the average case fatality rate of purulent meningitis is still greater than 20%. Due to the influence of many factors such as the proliferation of antibiotics, vaccination and the decline of patient immunity, the types and drug resistance of pathogenic bacteria of purulent meningitis have changed significantly compared with before. [0003] At present, cerebrospinal fluid (CSF) smear staining and bacterial culture are important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C12Q1/68C12Q1/14C12Q1/04
Inventor 王振海侯晓霖张旭侯玉婷马巧丽
Owner GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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