Microfluidic chip, device containing microfluidic chip and method for detecting or sorting samples

A technology of microfluidic chips and microfluidic channels, applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical equipment and methods, etc. Heterogeneity of individual cells can not be truly reflected, and achieve the effects of easy manipulation, low cross-contamination, and reduced reaction volume

Pending Publication Date: 2020-03-27
上海欣戈赛生物科技有限公司
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  • Abstract
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Problems solved by technology

[0004] In the research of cells, bacteria, and microorganisms, group analysis is used to obtain the average performance of cells, but the heterogeneity between cells and the individual performance of a single cell cannot truly reflect
Such observations and analyzes are generalized. If there is a special single change, it is impossible to detect it, and it is impossible to sort and capture a single sample.

Method used

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  • Microfluidic chip, device containing microfluidic chip and method for detecting or sorting samples
  • Microfluidic chip, device containing microfluidic chip and method for detecting or sorting samples
  • Microfluidic chip, device containing microfluidic chip and method for detecting or sorting samples

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Embodiment Construction

[0039] The invention discloses a microfluidic chip, a device containing the microfluidic chip, and a method for detecting or sorting samples. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0040] The components, raw materials and reagents used in the microfluidic chip provided by the present invention can all be purchase...

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Abstract

The invention relates to the field of microfluidic chips, in particular to a microfluidic chip, a device containing the microfluidic chip and a method for detecting or sorting samples. According to the microfluidic chip for detecting and/or sorting caught single cells provided by the invention, unicellular microemulsion liquid drops can be controlled to enter a cap-shaped chamber 3, so that each cap-shaped chamber 3 only contains one microemulsion liquid drop, a dual-purpose electrode 6 for detecting/catching is arranged in the cap-shaped chamber 3 or a cavity, whether the microemulsion liquiddrops in the cap-shaped chambers 3 contain cells or not can be detected, and thus sorted catching or a reaction can be performed.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a microfluidic chip, a device containing the microfluidic chip, and a method for detecting or sorting samples. Background technique [0002] Microfluidics, also known as Lab-on-a-chip, can integrate the basic functions of traditional biological and chemical laboratories on a tiny chip of a few square centimeters, including sample separation. , preparation, chemical reaction, detection and other operations. [0003] The microfluidic chip has the characteristics of controllable liquid flow, minimal consumption of samples and reagents, and increased analysis speed by ten or hundreds of times. It can analyze hundreds of samples at the same time in a few minutes or even less. And the whole process of sample pretreatment and analysis can be realized online. Droplet microfluidic technology is an important branch of microfluidic chip technology. Droplet microfluidic technology is dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34
CPCC12M23/16C12M41/36C12M47/02C12M47/04
Inventor 李珍仪蔡孟晏陆祎王竣弘
Owner 上海欣戈赛生物科技有限公司
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