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Using method of blood distributor

A shunt and blood technology, applied in separation methods, chemical instruments and methods, liquid separation, etc., can solve problems such as high membrane performance requirements, easy clogging of membranes, and mechanical damage of cells

Active Publication Date: 2017-06-13
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Membrane separation technology adopts non-cross-flow filtration, and the membrane is easy to be blocked. Using cross-flow filtration, although the membrane is not easy to be blocked, it has high requirements on the performance of the membrane and increases the cost.
Centrifugal separation technology is to separate the blood components from each other according to the difference in specific gravity through centrifuge separation equipment to obtain the required blood components, but it is easy to cause mechanical damage to the cells and easy to pollute, and the cells have phase changes, which affects the quality of the cells; at the same time , the environmental requirements of the laboratory are very strict, which increases the cost of cell separation

Method used

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Experimental program
Comparison scheme
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Embodiment 1

[0032] refer to Figure 1 to Figure 9 The shape structure of the invention, a method for using the blood diverter, the operation steps are as follows.

[0033] In the first step, select the fluid distribution channels of the main body 10 = 3 layers, and each layer has 9 distribution island walls 5 to form 8 sorting units, that is, there are 8 sorting ports 8 . Each layer of fluid distribution channels has a depth of 8mm and a width of 10mm. The drainage embankment 3 is 1mm wide and 6mm high. The diversion island wall 5 is fish-shaped along the direction of the fluid diversion channel, and its height is 8 mm, which is flush with the top of the fluid diversion channel; the end face width of the diversion island wall tip 20 is 2 mm. The width of the outer runner 7 of each layer is 2.5 mm; the height of the bottom 25 of the outer runner of each layer is 2 mm higher than the height of the bottom 23 of the inner runner; the bottom 25 of the outer runner is a horizontal bottom surfac...

Embodiment 2

[0039] refer to Figure 1 to Figure 12 The shape structure of the invention, a method for using the blood diverter, the operation steps are as follows.

[0040] The first step is to select the main sorting blood diverter as follows: the fluid diversion channel of the main body 10 = 5 layers, and 6 diversion island walls 5 in each layer, forming 5 sorting units, that is, there are 5 sorting ports 8 . The depth of the first layer of fluid distribution channel is 10mm, and the width is 12mm; the depth of the second layer of fluid distribution channel is 10mm, and the width is 11mm; the depth of the third layer of fluid distribution channel is 10mm, and the width is 10mm; The depth of the laminar fluid distribution channel is 10mm, and the width is 8mm. The drainage embankment 3 is 0.5mm wide and 5mm high. The diversion island wall 5 is fan-shaped along the direction of the fluid diversion channel, with a height of 10 mm flush with the top of the fluid diversion channel; the end...

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Abstract

The invention provides a using method of a blood distributor. The using method comprises the following steps: a first step of selecting a main sorting blood distributor with certain size and certain fluid distributing channel layer number according to the experimental and sorting needs; a second step of connecting a sampler to a total sample inlet; a third step of adding samples; a fourth step of detecting and regulating sample flow speed in real time; and a fifth step of cleaning the blood distributor after all samples are added. The using method has the beneficial effect of providing a cell separating device which can be quickly separate cells, is simple and convenient to operate, and is not liable to cause cell damages, and the cell device can separate while introduce liquid, and can perform multi-time circulating separation, so that separating efficiency is improved. Meanwhile, the invention further provides a using method used for the device.

Description

technical field [0001] The invention relates to a method for using a blood separation device, and in terms of use, it is a method for using a blood shunt suitable for ordinary medical institutions to separate blood cells. Background technique [0002] At present, there are roughly two technical methods for blood cell separation, namely membrane separation technology and centrifugal separation technology. Membrane separation technology uses a thin film with selective permeability as the separation medium. The raw liquid passes through one side of the membrane under a certain pressure. To achieve the purpose of material separation and concentration. Membrane separation technology adopts non-cross-flow filtration, and the membrane is easy to be blocked. Using cross-flow filtration, although the membrane is not easy to be blocked, the performance requirements of the membrane are high and the cost increases. Centrifugal separation technology is to separate the blood components ...

Claims

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Application Information

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IPC IPC(8): B01D17/038
CPCB01D17/0217
Inventor 徐文峰廖晓玲
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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